Oil Immersion Microscopy Animation

[@demonfedor3748]

Basic oil immersion explained quite well. I'm wondering if you have a video on advanced oil immersion. If not, advanced oil immersion happens when you not only use oil on the objective but also on a condensor,provided it's designed for it. It gives much higher resolution since there is no longer an air gap between the condensor and the specimen glass. However the objective's numerical aperture needs to be higher than 1 to take advantage of this technique. Also a dry condensor will always have it's numerical aperture below 1. Water immersion of a condensor is also possible,but it will give lower resolution. Best objectives I've seen had apertures as high as 1.45. I personally own an oil immersion APO 90x1.3 objective. Xylene is usually a solvent of choice to clean the oil away from objective lens, specimen glass and condensor.

[@glydellelozano9047]

Best explanation

[@Sara-iy3oz]

thank you

[@Tracks777]

Lovely content! Keep it up!

[@Tracks777]

When is your next video? :D Keep it up!

[@thebestplayerdead956]

So, do I still need to add a cover? Or no cover

[@Muqaatil]

Thank you! I really needed this!

[@terrylee5384]

i wish life could be explained this easily.

[@Zsofili100]

Thanks, great video. Congrats!

[@abilitylemur112]

Thanks. Greatly appreciated!

[@Tookrati]

Does the lense touch the oil?

[@khai6061]

yes it does

[@Tookrati]

Thanks! So to clean it, I surely need some special alcohol, don't I?

[@tanyatodd9660]

@@Tookrati Lens Paper & lens cleaner

[@SDbrc]

teşekkürler

[@adamdelarozza1985]

I would think its safe to say that the pressure in the oil is less than atmospheric pressure therefore its under a vacuum. Light refraction in a vacuum is the advantage and a poor man's SEM😄