Opgen Argus DNA optical mapping system part 1 - processor

[@Slimpickens45]

Used to work at OpGen in MD! Spent a ton of time with these machines. Very cool to see you diving in on this machine

[@gamerpaddy]

that stabilized camera is much better but it seems to record at some weird interlaced frame interpolation, theres a lot of jitter and double vision. fast movements are blurry. maybe look into the settings further. those recyclers are a gold mine for parts, most of them on ebay list their stuff for unreasonable high prices but with "make a offer" enabled and accept like 5% of the ask price. especially when its listed for a while.

[@mikeselectricstuff]

Yeah, but annoying when they don't enable the offer option. Some just have unrealistic expectations - I've seen stuff hanging around for years with no price drop. Need to look more into framerates etc. Partly why I did this as a fairly short vid. Don't really like the DJI though - no manual tilt adjust, and not good at focussing close, not helped by the screen being too small to see if focus is OK

[@agurdel]

5:15 That part is a really bad offender on the double vision. You should check if that artifact is in the source file or if that is caused by your cutting/postprocessing software.

[@christopherleubner6633]

600 pound for all that. A while back i ripped apart a PCR analyzer that used florescent tags that had a gargantuan MCP image intensifier hooked to a large fiber optic taper and a TEC cooled CCD. The CCD was mediocre copared to current versions but the 4 inch imsge intensifier assembly was totally unexpeded. Ended up using it as an image intensifier for a fairly large telescope. ❤

[@KallePihlajasaari]

Have you made a record of the tare down? Sounds like a great project.

[@jonlitchfield8888]

Lucky!! Its better than xmas when that happens

[@bigclivedotcom]

I wonder if the unit's cost paled against the ongoing cost of the consumables. It's very neat inside.

[@chrisdickens4862]

On this flip side, I wonder if that’s why these units sell for so cheap on eBay… Still working, but planned obsolescence via discontinued and proprietary consumables.

[@TheMrDefaultuser]

Obviously the business model is made around the consumables with these kind of medtech devices. But since it’s medtech they probably also charged enough for this thing as well😅 Not surprised to see you here Clive, love these teardowns

[@KeithRobison]

@@chrisdickens4862company discontinued this so consumables impossible to obtain

[@KeritechElectronics]

Always a joy watching you take apart some high end scientific or medical tech!

[@Psi105]

There is some sort of ghosting between frames, Probably happening somewhere in the post-processing workflow. Or maybe some sort of crude frame blending/interpolation is enabled. Could be unintended frame rate conversion going on between camera fps and KZbin upload file fps. You can see it by pausing on anything moving very fast, like a hand. Is not always visible in every frame, but if you step through a few frames using < > keys you will see it. Two frames blended into one.

[@NiddNetworks]

Hi Mike. Gotta concur with the other comments about the video quality... I appreciate it's difficult with something of that size - but the gimbal on the DJI isn't doing you any favours. Whether it's a setting or user error i can't say! This was a very tough watch, although the content is great - can't wait for next

[@SproutyPottedPlant]

It’s a free video of something interesting!

[@marty-h8r]

I just got food and sat down hoping for something good to watch while I eat. I didn't even dare dream this big. A fresh mikeselectricstuff, let's friggin go!

[@WolfmanDude]

These types of videos are the best on youtube, there is nothing else like it!

[@charliegarnish]

I didn’t realise how much I missed these videos until two were posted up!

[@jaro6985]

I bought one of those Parker vacuum pumps off Aliexpress, I'm sure it was salvaged from gear like this. It works well as a SMD pickup vacuum.

[@ToumalRakesh]

The sound is great. The side to side movement is kinda choppy though, maybe a bitrate or framerate conversion issue? Not sure.

[@WizardTim]

The sharpness and detail of the new camera is good, a portion of that may be attributable to the extra lighting from filming it there instead of the workshop. However there's a double vision effect, it looks like you might have recorded at 50 fps but then edited it on a 25 fps timeline but used "frame blending" as the interpolation method, this mode only really works when you have a long shutter duration, but it looks like the camera is recording at a short shutter duration so the motion is all weird and choppy. You can probably just change the interpolation method to "nearest frame" in your editing software which will fix it but I don't think you should try to increase the shutter duration to make it even smoother, firstly I don't think your camera will support it without an external ND filter but also it means the video will still be sharp when paused to read something. But really interesting teardown, the bio stuff is always very niche and interesting. Absolutely love that custom ducted heatsink for the TEC, wonder how much that cost to machine and finish.

[@AlexanderGee]

Yeah this is definitely an encoding issue. Lots of people who would be willing to help out with that Mike least we can do for the content!

[@yuribochkarev4477]

Finally! The beast teardown chanel back to the life after long interrupt Welcome back! 😄

[@DantalionNl]

Seems you might have a very long rolling shutter on the camera, the motion of the footage is getting pretty blurry

[@eliotmansfield]

“apple level aesthetic wankery” 😂😂

[@MrG-.-]

05:58 - The asset tag says TGAC site. After a 30 second Google search these machines likely belonged to The Earlham Institute in Norwich. The Earlham Institute was formerly known as The Genome Analysis Centre.

[@douro20]

I recognised what kind of air pump that was when I first saw it. It is a larger version of the one I bought a couple of years ago. It actually has a brushless motor in it. There should be a potentiometer in the top of it under a small sticker which controls the speed.

[@yyzkevin416]

I normally would not comment, but as you mentioned you recorded it differently, I somehow found the motion in this video difficult to watch.

[@DMSparky]

I was getting motion sickness. :(

[@f4c3l355]

Much better than before

[@calw2939]

Me too it seems like there’s a really long shutter speed set on camera. Maybe set into some sort of “low light” mode. It makes it a bit hard/nauseating to watch unfortunately.

[@fourtwo7612]

Me too, and focus seems terrible

[@eddhick]

The quality looks good enough but I think I preferred the old camera, this new setup is giving me a mild dose of motion sickness with something to do with the frame rate for sure!😂

[@martinday2815]

Hi. Don't see any jerkyness in video, more blurred or smearing when panning.

[@cliveramsbotty6077]

is that banana calibrated

[@mikeselectricstuff]

Look out for a sticker next time...

[@TilmanBaumann]

It's okay as long as the calibration is as old as the other equipment

[@SarahKchannel]

obviously yes, it seems an standard british imperial banana, since it curves to the right, unless the metric ones that curve to the left.

[@rchaffer]

"Apple-level Aesthetic Wankery" Poetry

[@dtiydr]

The company I work at have and also had a quite big and very complex biothechnical machine, not allowed to say what it was, and when it was bought 2017 cost around 210000 dollars. Other better ones had came out since then so we recently bought a newer one. The other machine needed a cassette type thing that you had use every time you start it up for very precise calibration and could be used around 50 times until it was literally worn out. That item was discontinued 1 year ago, 6 years after we bought it so the machine is now just a pile of scrap in the basement. It is how it is but its dirty. We have although earned so much money of it so we are on huge plus much over its value so its "ok" anyway, but it is still dirty.

[@Mister_Brown]

the motion blur the dji is causing on this video is honestly not preferable to the shakiness makes text hard to read sounds fine but i never really had a problem with your camerawork before

[@msylvain59]

It is even possible to find great stuff on ebay for 1 $ / GBP / €. Things I already had for that price: Nihon Koden blood analyzer, Alcon Allegro wavelight analyzer, military radios, several aircraft gauges and electronic boxes, grab bag of old photo equipment.... Perseverance, patience and a little bit a of luck are required, and of course you get what pops up, do not expect something specific, you never know what is coming next ! (just like when going in a fleamarket in fact)

[@glenslick2774]

Oh, you want the Apple level wankery treatment on your Fluidigm Polaris? That will be an extra $100,000

[@SionynJones]

An Easter treat, awesome thanks mike

[@nthdesign]

14:54 “Magic piece of thing that does all the clever stuff.” I laughed out loud at this! Seriously, though, that acrylic manifold is a work of art.

[@Actual_electrical_content]

To be fair , untill the banana got involved my perception of the devices was they were much smaller

[@fonkbadonk5370]

"Pneumatic ribbon cable" - a term I'd have never envisioned to refer to an actual thing!

[@KallePihlajasaari]

I first saw a similar product in some vertical 9 track computer tape streamers from the 1970/80's. The cool thing was they were rainbow colour coded like some ribbon cables. I think I still have one or two lengths in a box from 30 years ago, never found a good use for them as the bore was so small I had nothing that would fit in them. I vaguely recall that they connected various places on the tape drive to banks of calibrated pressure switches that would track the loading of the tape and movement of the acceleration loop. Now quite common in modern large format and inktank inkjet printers. From 4 to 8 bores depending on the number of inks.

[@fonkbadonk5370]

@@KallePihlajasaari We've actually had an HP DesignJet 1050C, which I dis- and reassembled fully. (Cleaning and belt replacement.) Not having to deal with individual tubes would have been quite welcome back then! =)

[@markhodgson2348]

I Would like to see you get your hands on an Apheresis machine my partner was hooked upto one as part of her cancer treatment

[@fghdfghdfghdfg]

Video was exactly what I hoped it was, mike taking it apart 👍♥️

[@DrGenestealer]

Unfortunately the video feels like it's melting

[@encryptededdy]

It looks like you're shooting at a frame rate that's different from your video editor timeline, and there some frame blending going on. If you're shooting at 50/60p, then upload at 50/60p.

[@robprupe]

Yeah it looks jittery especially when panning.

[@GothGuy885]

very interesting ! the parts that you harvested out of these items alone, are worth the money that you paid. if you were to buy these parts on their own, I imagine that they would be rather high dollar items.

[@mikeselectricstuff]

Big difference between what something would cost vs. what they are worth. A lot of this stuff is hard to even give away - who needs a 500uL dosing pump?

[@AB0BA_69]

@@mikeselectricstuffDid you just buy these two machines to take them apart and that's it? I mean, that's fun and it's your money, but also seems like a waste, no?

[@ikocheratcr]

@@mikeselectricstuff Maybe someone like The Thought Emporium might be interested ;), just my thought no idea if TTE is really interested.

[@memejeff]

Very nice system. Thanks for sharing. Quite intrigued by the usage of a standard nema17 stepper.

[@originalmianos]

I have some of those blue dual action air pumps from ebay. They are nice because they have a brushless motor and a pwm speed control wire so very easy to control. I use them for low vacuum. 20 bucks.

[@wolpumba4099]

*Abstract* This video showcases a haul of expensive biotech equipment, including a fluid analysis system and a DNA analyzer. Additionally, the video details the process of tearing down the analysis system to examine the individual components. The presenter offers insights into the functionality and design of the system. *Summary* *Sections* * *Introduction* * 0:00 Brief overview of the two biotech machines: a fluid analyzer and a DNA analyzer. * *Oil Injector Unit* * 0:54 Examination of a simple oil injector, used in high-power magnification systems. * 1:09 Functionality of the injector is explained. * *Mapping System Processing Unit* * 5:19 Exploration of the processing unit, including its control panel and internal components. * 5:48 Background information on the original cost of the unit. * 8:05 Functionality of the pneumatic drawer and the Peltier cooler. * 8:43 Exploration of the unit's internal hardware. * 12:52 Examination of the unit's manifold and thermal isolation setup. * *Mapping System Fluid Control* * 13:51 Closer look at the fluid control system, including valves and a piston volume control. I used gemini 1.0 advanced on the transcript

[@KallePihlajasaari]

@wolpumba4099 If you ask the right questions and give them the right data the AIs can produce reasonable output. How much formatting did you have to do to get it to lay it out afterwards?

[@wolpumba4099]

@@KallePihlajasaari The prompt I used is: Create an abstract and summarize the following video transcript as a bullet list. Prepend each bullet point with starting timestamp. Do not show the stopping timestamp. Also split the summary into sections and create section titles: ``````. For videos < 12 to 20 min (depending on how much is spoken) I can use a single prompt with gemini advanced 1.0 or gpt4. For longer videos I used to split the transcript into segments and merge the bullet lists (using the AI). But with gemini 1.5 pro I can get summaries of 1h30min long videos that also seem to be of better quality than merged summaries. KZbin comments don't support Markdown, therefore I need to change the formatting of titles from ## title to \*title\* and bold markup from \*\*bold\*\* to \*bold\*. Typically I do that in emacs with M-x replace-regexp ^\#\#\# \(.*\)$ → *\1*. I never had good results trying to explain the Large Language Model how to format youtube comments in the prompt. That is why I fix the formatting manually.

[@KallePihlajasaari]

@@wolpumba4099 Wow, I am rather in awe of how you have managed to coax so much useful information with meaningful formatting from the AIs. It does rather add value to the video if one comes back to it later and wants to locate a section. You could offer this as a service to video publishers and I deleting your comment above would make perfect sense to hold on to the small competitive edge you have now. Having this summary in the video description after the captions are generated would be a benefit to the publisher and you could find work for a number of cool channels. Thank you for your detailed reply, I did not expect the AI to do the formatting so well and thought you would have to suffer through much more of the grind.

[@wolpumba4099]

​@@KallePihlajasaariX It would be easy for google to provide this service, if they don't then I think they measure a reduction in their ad revenue. I think I have much less screen time now. After creating the summary I typically just jump to a few sections that interest me. I heard KZbin Premium users already can talk to an AI helper and ask questions about the video.

[@db9827]

Thanks for an interesting teardown as usual, but unfortunately the video quality is horrible.

[@Petertronic]

He's always used really crap cameras. It's the aesthetic of the channel.

[@noggin73]

Excelent! When can we expect a clone?

[@thelettera-yi2zz]

Weird. I'm in functional genomics and I've never heard of optical mapping. Neat teardown - nice to see the guts of these instruments, anyway!

[@electronbox]

"Apple level aesthetic w*nkery" 😀. New camera looks good, nice to see more more expensive lab gear torn down. Maybe I'll see some of it at Luton 🤞

[@meepk633]

They might have used the opposite side connector because the screens came with cables of a certain length.

[@Jeroen74]

Wondering why those manifolds are clear plastic, why not metal?

[@mikeselectricstuff]

So you can see they're clean inside maybe? Also probably easier to machine

[@kautzz]

corrosion resistance?

[@Jeroen74]

@@kautzz I did some research... clear acrylic (PMMA) is great for pneumatic manifolds because it's low cost, easily machined, and bonded using heat and pressure to build up a manifold out of multiple layers. Chemical resistance is not great at all actually. KZbin won't allow me to post a link unfortunately.

[@kautzz]

@@Jeroen74 doh obviously, thanks for checking. being able to stack layers makes things a lot easier

[@adriansmith31]

What do you do with all the equipment you teardown once done with it? I've asked a few youtubers about this but no-one commented. I've got a load of (mostly) junk that I can't resell on ebay and don't want to throw out. I've already done videos for them so not much use now. Other than salvage any useful parts for projects I just keep them.

[@mikeselectricstuff]

Excellent question - it can be a problem for those of us who can't bear to bin useable stuff. There's an electronics car boot sale ever year near Luton ( google ddrc bootsale, usually May but late June this year) - I often take a car ( occasionally van) -load of stuff there and anything that doesn't sell in the first couple of hours goes on the "Free stuff" pile. Last year I only came back with one small plastic bin of stuff that was too good to give away. There are also radio rally/hamfest type events elsewhere in the country - can't comment on which ones are best but definitely go for the outdoor bootsale type rather than the indoor ones.

[@adriansmith31]

@@mikeselectricstuff cool, thanks. Luton is a bit far from me but there's a few car boot sales near me. One in Bradford (mostly tat), a big one near Leeds / Bradford Airport but always very muddy. Mostly market traders and shifty looking blokes selling likely stolen tools and shed contents. Another at Harrogate (posh tat) occasionally pick things up at these sales. Not tried selling there yet. Might try selling at the Airport one if weather good this summer.

[@mikeselectricstuff]

@@adriansmith31 I doubt traditional boot sales are worth it, though you may get the occassional techie type there, or perhaps a few clueless civilians who think tech stuff is valuable. - Ham radio events are a much better bet. Google UK radio rallies for listings.

[@adriansmith31]

@@mikeselectricstuff yeah you are right about the traditional boot sales. Sometimes can get some bargains if you go early when site opens. Once bought a pallet of server parts; it's cheaper to buy a entry level server and then upgrade rather than buying a high end pre configured one. The brand new parts were then thrown out. A long time ago but got a load of DDS4 DAT tape drives for a fiver each. Made over 4 grand reselling those. But I'll definitely search for ham radio and similar events.

[@thomasjohnlorren]

There’s some kind of motion blur happening with this video that’s not usually in your videos.

[@gregorymccoy6797]

The camera works well and the audio is more than acceptable. I tend not to notice camera issues as I focus on the devices themselves. I've a fair idea that device you dismantled is too complex and could be factored down to something more reasonable today. I wonder how much competition they had or if labs even care about cost of equipment,

[@feenixb1o7]

Which carboot do you take this stuff to? What's your eBay link?

[@mikeselectricstuff]

google ddrc bootsale, June 30th this year, not sure I'll be selling this year yet. ebay name same as YT channel

[@unmanaged]

you need to take those nossles and use it to create drips, make a 50/51hz strobe and make the water look like its going in reverse...

[@unmanaged]

would look good on the desktop

[@Neelsworld2022]

Whats your opinion about the Opgen Unyvero ?

[@jaymzx0]

0:26 Sweet Bloodywood shirt 🤘

[@Lion_McLionhead]

The equipment went from 1/2 mil to 0. The back yard went from 1/2 mil to 1/2 bil. Typical inflation targeting.

[@jonlitchfield8888]

Absolutely spot on!!

[@bugrats]

Have you ever found some that you never knew existed that you then later purchased for a new project

[@seifinavid6798]

Hi there how are you thanks for your informative data I want to as a favour Can you drive for example manifold and explain more about it I'm willingfully following you and your channel

[@69uremum]

The video looks strange, there are some weird artifacts due to that gimble and camera setup.

[@NeungView]

13:42 I beg your pardon!

[@tekvax01]

The autofocus kept on hunting back to your hand. also, you might get a better recording if you used a tripod, and moved the parts and not the camera so much. However, the image stabilisation was better than the interlacing of the fields ripping apart when you pan around. Panning slower will give you less distortion in the image as well. I always enjoy these big pricy equipment teardowns! Keep up the great work Mike!!

[@TheRasteri]

for some shots it can be helpful to record footage seperately from voiceover. Means you aren't multitasking finding the best shot while trying to come up with something clever to say. More editing involved though...

[@mortlet5180]

I've got *really* bad motion jerking and doubling at 1x speed, such that it is actually unwatchable, with a building headache and nausea that feels identical to when I tried to watch movies in the cinema. Just changing to 1.5x speed is already quite a bit better though and 2x is almost completely fine. I'm watching on a Samsung tablet with 120Hz mode enabled. PS. Just checked on my pc, using SVP set to interpolate 5x to 120 fps on a 240Hz OLED (even that is a bit much for unbuffered realtime playback on an OC'd 13900KS @ 6.3GHz with a RTX4090 OC'd to +142 core and +1400 mem) and everything is great with my only nitpick that the interpolated frames look a bit blurry, but that's to be expected during motion at such a low original frame rate. Maybe you could consider shooting at 60 (or even some glorious 120) Hz?

[@NeungView]

I bet you're a hoot at parties.

[@MarcusGarfunkel]

Yep, video is horrible 😢

[@memejeff]

Extremely cool

[@DrTune]

1994 called and they want their VHS camcorder back ;-)

[@SproutyPottedPlant]

Well they can’t have it because the video was free to watch and and contains interesting items.

[@RicoElectrico]

Babe wake up, the tired Brit dropped a new teardown ❤

[@TilmanBaumann]

Video quality is great. Even on close ups. Also mice mancave shed

[@unmanaged]

we never get any good deals like that here in the USA everything the resellers sell for is huge prices ....

[@Mister_Brown]

best offer, i've paid 2% of the asking price before on some stuff, just look for stuff that's been listed for over a year and has little commercial use

[@jb5631]

In the US there is a lot of great stuff to find I think, at least much better than in the EU country I'm now. Here some countries have a lot of good stuff while others have barely anything. Have you tried going to some private scrapyards?

[@markglover2525]

I flipping hope you washed your hands for 20 seconds after handling all that Mike; it might have been used to prepare lab samples of 'Super Gonorrhea'. Or worse; fillings for Findus Crispy Pancakes.

[@TwoHeartedSpider]

What?

[@Andrew-rc3vh]

Those people who previously owned it must have too much money if they can afford to chuck £700k's worth of rip-off machinery away. It could be a case of one of those biotech firms charging mega bucks for drugs, or the other thought is the NHS. They are the largest money sink in the UK.

[@christopherleubner6633]

The maker will discontinue the consumables for these machines making them a collection of expensive scrap metals and parts.

[@Andrew-rc3vh]

@@christopherleubner6633 Ah, I thought there must have been a reason. It's like doing business with the devil.

[@KeithRobison]

@@christopherleubner6633company is basically bust & this instrument discontinued in 2016

[@TH-wr1dv]

if you want comments of your camera. It is just absolute trash. Make me nausea. its 15fps or what?

[@Tadesan]

Makes me sad that you get to be happy.