PCR types in Hindi |colony PCR, nested PCR, inverse PCR, real-time PCR, rt PCR

Pcr types in hindi - This lecture explains about types of Pcr. Different types of Pcr technique discussed in this video including the following -
Colony Pcr, nested Pcr, hot start Pcr, inverse Pcr, rt pvr, real-time Pcr, quantitative Pcr, qpcr, touchdown Pcr, inverse Pcr and many more types of Pcr.
Stay tuned to know more about polymerase chain reaction types.
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Thank you for watching the molecular biology lecture on Pcr types and examples.

[@monikasingh6839]

Beautifully explained

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You are the best Sir! I salute your efforts towards teaching us with such integrity❤

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[@drshinwarishinwari1253]

I like your Hindi you explained very well in Hindi 😍👍🏻

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@urbashisharma3893]

Sir I love you soo much ...mne bahut wait Liya ki kB aap hindi m daaloge...am soooooo happy ..thanks

[@shomusbiologyofficial]

Glad I can make you happy to learn

[@sanjayagarwal2339]

Sir you are amazing ...thank you sir

[@narottamsahu1435]

Thank you sir very nice explain in hindi ,, 🙏🙏

[@ananditajha6718]

Really superb... Mr Suman. God bless you 😇

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@snehak7582]

I am so used to your english videos that its weird to watch you explaining in hindi!😂

[@totalgaming5792]

Yas

[@manishakhewadia6133]

Awesome.....thnks....aapke videos bhut achhe hote h nd helpful hote h👏👏

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Glad to hear that you are getting benefit from the videos

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You’re the best sir!!!!!!!! You should get prize in biology 🧬

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yes..he should be awarded

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Yes indeed itny difficult topics itny asan tareqy sy explain kiye ha

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He has csir net qualified

[@shadabkarim216]

It was explained superbly❤❤❤

[@swatichauhan2531]

Excellent job sir👍👍👍 You have good technical knowledge .You explained PCR in such simple way that I am finding easy to learn all this.Your hindi too is good.

[@shomusbiologyofficial]

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[@pragyasingh6445]

Thanku very much sir for all videos

[@sweta8135]

Thanku sir.. It's really useful.

[@shomusbiologyofficial]

You're welcome

[@shikhakumari6575]

Sir, kindly make a tutorial on lymphocyte trafficking.. I really need to understand it..

[@rishabhthakur3711]

Gjb sir

[@deepikadev9361]

thanks sir hindi vedio bnaane ke liye

[@bindjoieiiouieasingh7029]

Thankyou so much sir

[@ayushisamriyar4732]

Very well explanation sir.. Clearly understood by anyone.. Your way of explanation is so clear nd smooth... Thnku so much sir...

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@ayushisamriyar4732]

@@shomusbiologyofficial sure sir..

[@ekramul3338]

Thank U sir........

[@radhaagarwal9407]

Hats off 🙏🙏🙏🙏 sir thanks for this video

[@meghavatsa8191]

Very well explained 🙏🙏

[@shomusbiologyofficial]

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[@xubivet3295]

Sir itna easy bana diya❤️

[@shomusbiologyofficial]

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Sir your teaching is just incomparable to anyone ....please can U explain ITS region Nd related topics

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@masroorkhalil9655]

sir your hindhi is best ever

[@shomusbiologyofficial]

Thank you

[@masroorkhalil9655]

Shomu's Biology si pls make a video on how to write thesis in scientific writing pharaphases in hindhi pls pls pls sir

[@sayantansaha53]

Greatest ever explanation Sir.

[@shomusbiologyofficial]

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[@sayantansaha53]

Welcome sir

[@samgaming91632]

Best of the best

[@shomusbiologyofficial]

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[@rishtanandinisingh4526]

THANK YOU SO MUCH SIR ! U R THE BEST BIOLOGY TEACHER ON KZbin

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@pabitraamadhurima6960]

In the case of inverse PCR, if the sequence at the end are unknown then how do we identify the desired restriction enzyme to cleave and form sticky ends prior to the formation of circular DNA?

[@SHUBHAMSINGH-gh6ln]

Same question

[@sushreemonali36]

Salute to you 👍👍👍🙏🙏🙏🙏🙏

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[@amanagrahari12]

too good sir

[@shomusbiologyofficial]

Thank you

[@mamtarathore6641]

Very very nice 👏👏

[@shomusbiologyofficial]

You're welcome

[@vasundhararaina6849]

Moreover in inverse PCR the DNA molecule used initially is basically wat a whole genomic DNA or a small part of it. Coz of its whole genome then adding a restriction enzyme may produce several small segments. How will we then isolate the desired one to be used for inverse PCR.

[@nikitavatandar1666]

very nice vdo loved it

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[@biologyc554]

Nice video sir.

[@shomusbiologyofficial]

You're welcome

[@deepikamahto8039]

Nice explanation

[@shomusbiologyofficial]

Thank you

[@aboyfromhills2559]

First to watch. Thanks for every videos sir. I am going through your videos all my semesters. ♥️

[@shomusbiologyofficial]

Glad you liked my lectures

[@tarannumkhatoon5070]

such me bahut hi achi vidio hai wo bhi hindi me

[@shomusbiologyofficial]

You're welcome

[@sahilprabhakar4788]

Sir plz give complete information about ICAR - ARS exam

[@letstravel8840]

Plz presentation on blast and fasta

[@jyotisingh448]

Sir as we designed the second set of primers for the target dna in nested pcr why can't we designes the specific primers of the target dna for the common pcr.....sir kindly answer i really wanted to know

[@zulqarnanh]

Awesome

[@shomusbiologyofficial]

You're welcome

[@gauriargulwar6035]

U speck very nice hindi,

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Thank you

[@Chia5555]

Thnku so much sir

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[@mukeshmurmu471]

Thank you sir achha hai

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[@avinashkumar-ow7pq]

Thank you for explaining the things in Hindi

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You're welcome

[@ImranAhmad-df3mi]

Thanks sir

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You're welcome

[@aayushipathak6666]

Thankyou so much sir 🙂

[@shomusbiologyofficial]

You're welcome

[@arkhamyt6052]

Sir in nested PCR , then what is the need of normal primers? we can directly add the nested primers on the target sequence and amplify our desired gene. Then what is the need of normal primers then?

[@anshusharma2438]

thanks you sir👌👌👌👌

[@shomusbiologyofficial]

You're welcome

[@user-vg6wm3fy1e]

Excellent efforts...special thanks❤❤❤ i have one question, where does the primer attach the DNA strand in reverse PCR??? Because when known sequence is in mid, primer is attached at its sides and continues to add nucleotides further next to itself in the direction of known sequence but now the known sequence is at ends so how will primer be attached???

[@hemmahur1891]

Sir please make a video on Carrier detection in Haemophilia A PATIENTS by using linkage markers

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Nice sir

[@shomusbiologyofficial]

Thank you.

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Awsm sir ...thanku so much jo ap hm logo k liye itna struggle krte hai ...thanku sir ..God bless u Sir mujhe apse ek Favour chahiye plzz help us ... Sir microscopy ke kuch video ap jaldi krne ka try kr skte hai ... I m sure apki list me to hoge hi ye topic but me thoda jaldi krne ka abhi favour mag rhi hu ...i hope u accept my request..thanku sir

[@shomusbiologyofficial]

I will make many more videos in hindi

[@ART-fs2wd]

Wow Sir your hindi 👌👌👌

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You're welcome

[@sandeshhulgunde5709]

Nice hindi sir

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Thank you

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thankyou sir

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You're welcome

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Nice video sir ekad uhi hindi m b video bana dijiyega

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Thank you. Glad you liked my lectures

[@sidrakhan8589]

Your Hindi/,Urdu video are much more good for understanding. Even though English are also v well elobarated. What about starting a separate new channel of shomu biology Hindi

[@shomusbiologyofficial]

Thank you

[@yogeshanjansinger2872]

Thanku sir

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You're welcome

[@davidsweeney111]

Shomu could you make this video in English as well please

[@shomusbiologyofficial]

It's already there

[@bhagyashreechatterjee959]

Aamr akhon Mone hoi.. amr jibone zoology r kono chapter e r kono somossa hbena .. karon shomus biology ache ✌️✌️✌️✌️

[@shomusbiologyofficial]

I am glad to hear that you're getting benefit from my lectures. Stay tuned

[@rubabikram4619]

if no plz tell the difference between nested or hemi-nested pcr

[@arjitakaushal8116]

Sir... Please make a video about how to help pcr for diagnosis disease

[@shomusbiologyofficial]

Okay sure

[@rubabikram4619]

is hemi-nested or semi-nested pcr same?

[@anilkohali6198]

thk u sir

[@shomusbiologyofficial]

You're welcome

[@tanvisharma812]

Sir i have a question related to nested pcr.. That we are adding both outer and nested primers in a definite concentration... It is true that outer primer will amplify the unwanted DNA and nested one will amplify the target DNA.. But the whole process is going on for 30 cycles lets say... And the unwanted DNA along with outer primer they too are in the reaction till the reaction ends for 30 cycles how come we are going to identify wheather the unwanted DNA is not amplified as the reaction has outer primer and DNA till the end.... I mean are we adding any enzymes that will. Specifically cut the target DNA from the rest unwanted DNA

[@rishiivirgo]

by gel electrophoresis. best example of nested PCR is identification of Rotavirus

[@sunilkumarsahu2283]

Sambhu bro I love you

[@shomusbiologyofficial]

Thanks

[@pinshusamariya7873]

Kya PCR hiv test me bhi kaam aata hai, or iski accuracy kitni he

[@punambardhan9147]

I want to understand Degenerate PCR primer designing..Could you??

[@ilmasiddiqui110]

make ur profile on instagram too sir ...so that more people get to know about you ...and get benefited by watching ur vdos..

[@shomusbiologyofficial]

Sure

[@juwairiahmazhar9607]

👍👍👍

[@Prabhjotsingh-fs3vp]

Thanku sir make a video in Hindi medium ..... I wish u will make a video Hindi in future

[@shomusbiologyofficial]

Sure

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Please suggest any book of molecular biology i m bsc mlt student

[@indranisahu5527]

Nice sir ji 🤗🤗 PDF mil sakta hai Kya

[@tarannumkhatoon5070]

👌👌👌☺

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@sonamdhanava3098]

Will I eligible for UGC net exam after the M. Tech in cosmetics..?

[@sunilkumarsahu2283]

Texonomy k upper video upload karo please

[@shomusbiologyofficial]

I will.

[@prabhudasnair5636]

In Rt pcr why rna primer is used instead of dna primer? While in other type of pcr dna primer is used Please reply

[@Vanshikajain2]

If in the question paper given RT PCR then it is real time or reverse transcriptase PCR ???

[@BTS_army_V30]

Hello sir . semi-nested pcr pr b vedio bna de .iska concept clear nhi ho rha

[@hiteshpegu2844]

Can we use same pcr for testing dna of different species i.e human ,plant ,bacteria

[@shomusbiologyofficial]

Different chambers

[@devarshkumar9955]

what type of polymerase we use in real time PCR as in taq man probe it uses exonuclease activity

[@shomusbiologyofficial]

Probe or dye

[@vasundhararaina6849]

Nested always keeps me wondering ABT it's need. Wn one already has the inner primer set then y to use the external one. Anyway practically things are not so common wd the theoretical aspects.

[@rekhasaini6753]

Same here.... I am still not able to understand nested PCR 😢

[@anmol786]

Sir aap BSC zoology honours ki whole video hindi me bnao either payed video ya free depend on your .....bt mghe need h kaise hoga pls help me sir..

[@nathadil6765]

Sir, can you plz upload csir life science videos

[@shomusbiologyofficial]

Many videos are uploaded. There are more than 2300 videos

[@rambhawan2643]

Evolution video in hindi please sir

[@jaidtechy2762]

Sir notes mil saktey hai?

[@gautambharali4492]

Late PCR kya hai please video sir

[@shomusbiologyofficial]

Okay

[@rajarora4592]

RNA RT-PCR is confermetory test and target not detected means

[@kumaresangomathykumaresan7817]

Can you make videos in english plz

[@shomusbiologyofficial]

You will get this video in English in my channel as well

[@tarannumkhatoon5070]

sir ap pls hindi me bhi kuch vidio banyakariye pls ☺

[@shomusbiologyofficial]

I am making more videos in hindi

[@glowupwithnature145]

Why we don't use just nested primer ? Why we use 1st primer if it binds unwanted sequnces?

[@shomusbiologyofficial]

One primer set can make more unwanted amplification. Two set can reduce unwanted amplification

[@yashpathology]

Flanking sequence means sir

[@shomusbiologyofficial]

Means the sequence present as single stranded region of DNA

[@anuragsingh-hp4ko]

I think Ye topic eng m cover ho gya h?

[@shomusbiologyofficial]

Ues

[@ilatiwari2807]

Sir ,types of PCR ,csir net me pucha jata h kya?

[@shomusbiologyofficial]

Yes

[@sandyrocks7866]

Sir agar iska test kit research purpose ke liye he but diagnosis purpose ke liye nahi he to aaj ki date me isko diagnosis ke liye kyo use kar rahe he

[@shomusbiologyofficial]

There is no such thing as only for research purpose if it is used in research, and found to be effective, we must use them for diagnostics every single diagnostic test that we see these days were sometimes a part of research work

[@kolasrinivasarao1096]

PCR English plz

[@shomusbiologyofficial]

Already there

[@pumscwala]

Thanks.sir ..Jo biology hindi ki Play list hai usame Abhi PCR KA topic nahi show Kat raha hai plz. Two lecture.

[@shomusbiologyofficial]

I will add

[@pumscwala]

Shomu's Biology sir Abhi play list me nahi Add Hua...plz...

[@Dineshsharma-qp6os]

Sir hindi me hi video banaya kro