qPCR details | quantitative real time PCR | RT PCR | Biotechniques

[@quickbiology5159]

Superb Arpan i have joined PhD lab and your videos are helping me when i was teaching, they are still helping me in research ,you are a person to whom i would definitely would like to meet you one day..

[@animatedbiologywitharpan]

Thanks a lot. I am really happy to help

[@cutieaadi9599]

Please don't forget to mention in acknowledgement!!!

[@emeraldradiancemadinamunawwara]

I LOVE you! This is the best crash course in RT qPCR on the entire internet! God bless!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@emeraldradiancemadinamunawwara]

@@animatedbiologywitharpan Sure thing!

[@akamontazysta8104]

Wow, I am amazed how gracefully, briefly and simply was this explained. Exactly what I needed, thank you very much!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@akamontazysta8104]

​@@animatedbiologywitharpanOh yes I will!

[@ghodbaneamira8228]

best explanation ever, thank you.

[@animatedbiologywitharpan]

Please share with friends

[@cyrahkingsley2187]

this is the best qPCR explanation video ever!!! Especially the part about CT, I was so confused about the term but your explanation really helped :)

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@animatedbiologywitharpan]

check out our biotechniques playlist. we explain things in simple terms

[@sabaihorti]

very clear explanation, many thanks

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@juliamzyk4368]

Great work, thank you for the explanation!!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@Someonrr4458]

It is realy a fantastic video.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@satheeshrajah96]

Really informative plz do real time analysis demonstration which help us grasps more about this

[@animatedbiologywitharpan]

that part is a payable service which we provide @250 rs

[@ShambhaviTrivedi-ub2yi]

can you please shoot a video on how to plot graphs/ curves of different datasets using graphpad prism.. Really need to learn this

[@animatedbiologywitharpan]

there is a course on this in my website. Launch date 5th April. It's only 250rs. email us at [e-mail] such that we can send you link.

[@RITIKAjuvenile]

Most adequate videos of experiments

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@RITIKAjuvenile]

@@animatedbiologywitharpan yes of course

[@RITIKAjuvenile]

@@animatedbiologywitharpan i am also from medical research

[@ominibioentrance6458]

Good explanations........what if the ct value is same for the control and treated sample. Say ct vlue 26 for both. Does that mean in gene expression or the transcript level is smae in both control and treated sampke

[@satheeshrajah96]

If you can plz advice us how to do mrlt curve analysis

[@fatmabetul5985]

Sir, You are amazing at explaining the lab techniques' protocols and principles. Thank you for your help and the effort you pay for us! My best regards from Turkey!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@fatmabetul5985]

@@animatedbiologywitharpan it is my pleasure. I will do my best to help you!

[@kiklly]

Thank you so much.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@bellebelle9789]

Thankyou so much for your video. Can I ask… if my no- RT control showed a peak around 75 does this mean contamination or primer dimers? Thankyou!

[@animatedbiologywitharpan]

@@bellebelle9789 most likely primer dimer

[@bellebelle9789]

You are the best THANKYOU ❤❤❤

[@Universe12355]

Can you please do a video interpreting further CT value and melting curves and how to interpret them? By saying for example this CT value with this Tm indicates primer dimer …. Or For example, what would a Ct value of 39 and a Tm of 64 mean when found in a negative control ?

[@animatedbiologywitharpan]

I will try

[@Universe12355]

@@animatedbiologywitharpan thank you so much!

[@milenlozanski456]

Major tool of my BsC and MsC thesis! 🧬🧪🧫

[@animatedbiologywitharpan]

Glad to hear that. I hope this video was good in explaining

[@joshuastavos4376]

Can you explain the disparate CT values for the 18s RNA control for the Control 1-3 groups and the Mut1-3 groups? Is it because there is more 18s in the Mut1-3 groups ( possibly due the different intracellular environment in the Mut 1-3 groups??). I would have thought that the 18s RNA level would ve comparable in both groups at the outset.

[@SneufxWjehfyfr-v9z]

Garcia Angela Thompson Laura Lopez Daniel

[@alhassanmohammedawal3851]

can you explain to me mathematically, how the CT values decrease with increase concentration.

[@animatedbiologywitharpan]

The Cycle Threshold (Ct) value in quantitative polymerase chain reaction (qPCR) is inversely related to the initial amount of target DNA in the sample. A lower Ct value indicates a higher concentration of the target DNA in the starting material. In qPCR, the amplification of the target DNA is monitored in real-time, and the Ct value is the cycle number at which the fluorescence signal crosses a predefined threshold. The fewer cycles needed to reach this threshold, the higher the initial concentration of the target DNA. So, a high DNA concentration in your sample would generally result in a lower Ct value, and vice versa. Researchers often use Ct values to estimate and compare the relative abundance of target DNA across different samples in qPCR experiments.

[@alhassanmohammedawal3851]

thank you Do you have experience with Quant studio5? @@animatedbiologywitharpan

[@ritabrataroy9797]

Your videos are just great...can I ask you a sort of personal question if you don't mind? How much time are you expecting to get your doctorate degree,..as I am entering in the research world,I want to know because most of the institutes take more than 6 years generally, previously I thought that 5 years is enough.

[@animatedbiologywitharpan]

I got MSc + PhD in 6 years

[@mithilasandeepa8999]

😅😅😅😅😊😊😅😅

[@sarahjustme]

At my institute a PhD takes 4 years and often extends to 4.5 years, but not longer.

[@MargariteSoles-n1e]

Blair Squares

[@enriquecorona3184]

Explantions are good to understand, however would suggest that speaking slower the understanding of your saying would be better.. thanks for posting the video

[@animatedbiologywitharpan]

Thanks for your suggestion.Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@AnnaWilliams-b7n]

Marvin Bypass

[@omarosama8934]

Where is the paper in the description?

[@zeinabkouaider]

Do you know how to calculate the percentage of GMO in food using qPCR please?

[@animatedbiologywitharpan]

I Don't think it's possible

[@FrederickHugo-n8s]

Huel Courts

[@TheAmrein]

why is the 18s the one that varies so much? isnt it supposed to remain constant?

[@animatedbiologywitharpan]

This was a hypothetical data

[@joshuastavos4376]

@@animatedbiologywitharpan you kind of answered my question which I just added, but I ask almost the same question for confirmation.

[@abhiruchisah1346]

where is the paper link?

[@animatedbiologywitharpan]

livak et al 2001, pubmed.ncbi.nlm.nih.gov/11846609/

[@niraxlevi9930]

Absolutely amazing ❤wow i watched many videos and i didn't understand it you saved my *ss 😂 also i have a question, the DNA fragments that are undetected aren't measured? Let's say for the sake of simplicity, they are 50 undetected ,their number isn't added ?

[@animatedbiologywitharpan]

very unlikely the amplified DNA molecules would not be detected because the sybr green dye binds to dsDNA pretty strongly

[@ZangwillLaura]

Johnson Jason Harris Melissa Thomas Linda

[@yasminemaanani8215]

Merci !

[@DevonPhoenix]

Not diagnostic

[@animatedbiologywitharpan]

Why do you think so ?

[@DevonPhoenix]

@@animatedbiologywitharpan the person that invented it carey mullis stated that. But maybe im wrong i dont know.

[@drsarathkrishnanmp8449]

Diagnostic role is also there

[@sandeepb9397]

Full Benglish !

[@animatedbiologywitharpan]

Unlike others i flaunt my Benglish accent. If people love the french mixed english then whats the problem with Bengali.

[@aherodoingcriminalactivity]

​@@animatedbiologywitharpan Your Benglish very easy to understand and comprehensible for me. Thank you brother 😊

[@soudas08]

@@animatedbiologywitharpan proud of you…

[@rb11ism]

Not good. Its not explanatory. I spend more time in explaining what is RT-PCR and less for the explanation of CT values. Really disappointed listening to your video. You must improve.

[@animatedbiologywitharpan]

thanks for your comment, we will improvise