Peptide Synthesis in the Laboratory

[@maeve6714]

This is hands down the best (and one of the only) videos I have found about this. Thank you SO much, you’re getting me some much-needed marks for my midterm two days from now!

[@mevansthechemist]

Hope it went well, Maeve! I remember thinking when I made this that it seemed like there was a gap in what was out there, at least on KZbin. Thanks for your kind comment!

[@MsRuru1]

Much better than my professor's attempt to explain all this. Thank you so much ! it helped me a LOT

[@MeistroJB]

I selected SSPS for my lab final presentation and until now the wading has been knee deep. But now, you have explained the process so clearly, much better than anyone else, I might be able to convince the class that I actually understand it too! Thanks, Professor Michael!

[@19cavalier93]

I thought the Chemistry God was talking to me from 3:44-3:57

[@gunnarvance4385]

instaBlaster.

[@mevansthechemist]

Whoa. I think I got a little adventurous with audio effects there. :-P

[@emancan8]

you explained this so well. Thank you so much for your hard work in making this informative video!

[@dr.kushalgrakh7520]

Hii there, nice video. I am bit confused on my synthesis as I got a sequence using phage display library against a pathogen for example: STFSQNG (N to C) and I want to synthesise it using Fmoc SPPS. But as the FMOC SPPS using Rink amide resins generate from C to N, should I use Glycine as first amino acid (reverse) or use S (serine) as first amino acid and then T,F,S etc.

[@sonatinac8514]

Do u write book,I need them for revision

[@marinasoliman2413]

So clear! Thank you!!

[@darkjavierhaf]

Really nice explanation! Thanks a lot! Just one more comment. Consider that each reaction has a limited efficiency. For larger peptides, final purification remains very little product

[@mohamedibrahim5100]

Is this method applicable in reality. I have a tripeptide made of isoleucine alanine glycine I want to synthesis .what is the easiest and cheapest way to do it.

[@mevansthechemist]

It most definitely is, although DCC is hardly the ideal coupling agent. Look at PyBrOP, EDC, and other peptide coupling reagents. DCC will likely be the cheapest, but it's messy (from personal experience...). :-)

[@roheenasiddiqui5267]

plz can u ask the reference of this topic kindly

[@brittany0wills]

Thank you so much!

[@kinirabeach4288]

amazing video...

[@SK-wo2qg]

WOWOWOWOW YOU EXPLAINED THIS SO WELL TO ME IN 6 MINS! TOOK MY LECTURERS MONTHS AND THE CLASS STILL DIDN'T UNDERSTAND

[@sonatinac8514]

Or tutor?in urgent need,will contact via twitter

[@traxxasbro5604]

You should just leave the methyl groups as plain sticks...it’s a little busy looking when you label every methyl as “Me”, and I’ve honestly never seen anyone do this before. To each his own I suppose.

[@s.lindburg8214]

People can be so ungrateful.

[@traxxasbro5604]

Lil Jaded-Bunny it’s merely a suggestion to improve understanding for those of us who use the standard conventional method of drawing skeleton structures in Organic Chem. Calm down you precious little snowflake.

[@traxxasbro5604]

Lil Jaded-Bunny furthermore, if you can’t take constructive criticism, you’ll be absolutely useless in any professional scientific community, especially within the field of chemistry. Clear communication is absolutely essential in Chemistry, and deviations from the conventional standards of nomenclature can lead to unnecessary and completely avoidable confusion and mistakes. There’s a reason why IUPAC exists and why there’s unified rules on how to draw and name structures...