Protein Analysis: The Bradford Assay & Spectrophotometry

[@jasonwo939]

Not often does university level educational content come at this of a high quality and absolutely free. The camera work is immaculate, the sound design relaxing, and the storyboarding ingenious. Biochemistry is one of my favorite disciplines of science and I am sad that not many people will see this masterpiece of modern education. Wish you the best

[@BioLabCollective]

Thanks for taking the time to comment! Science is not what people relax to on KZbin, still finding the best format. More to come soon

[@SBRay-ir2cp]

Very clear. Thanks.

[@jhustinecardenas9609]

A very helpful explanation, would you be able to talk about the Modified Lowry Method Assay and procedure?

[@alisniper797]

Great video! Really helped me with my lab report : )

[@BioLabCollective]

Great to hear - thanks for watching

[@yushi.hoseok]

How much of Bradford reagent should we add?

[@BioLabCollective]

Varies depending on the kit you buy. Should be the same volume of Bradford reagent in every tube, because the variable you are changing is protein concentration

[@osmirod1]

How do I decide the concentration of protein I should load per well when running my western blot? Help!

[@aimeecaroltangonan4472]

Great video. 😮 Sir, what could have gone wrong if blank (control) has a higher reading (O.D.) value than the standard but they have same color visually.

[@BioLabCollective]

Your blank has been contaminated? The reading should be a lot lower

[@SBRay-ir2cp]

Sorry ! Is there some software or algorithm for drawing the standard curve ?

[@BioLabCollective]

Nothing fancy, just Microsoft excel or you can draw it by hand.

[@SBRay-ir2cp]

@@BioLabCollective Thanks very much

[@saharkhalil5483]

My concentration is 0.3952mg/ml how can its calculate its volume to load maximum 50ug of protein per well?

[@BioLabCollective]

We go into this topic here: Laboratory Solutions and Dilutions kzbin.info/www/bejne/qmPIdJKeq8Z8eMU

[@Jake_M_Warren]

concentration = mass / volume

[@manobb403]

good vid but you did too much cutting while edditing, it seems too fast to understand

[@BioLabCollective]

Thanks for the feedback. Unfortunately slow videos have low watch time and the algorithm downvotes them on KZbin. Will try to make every video better