Practical Aspects of HPLC Method Development

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Very nice video lec..more important and helpfull..thankyou 🎉

[@dr.jitendrajharbade5221]

Excellent lecture, thank you to all team members.....

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[@ojaswighadge7435]

Very very excellent Dr. Sonal. Thanks for such a useful piece of information.

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[@swarnalatha1451]

Excellent explanation thanks 🙏

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[@PharmaMedicinalChemistry2021]

Excellent & highly useful presentation on HPLC, Thank you mam.

[@bhargaviyatham6303]

Excellent very easy to understand and who prepared for newly in interview purpose will deffinately help ... Thank u for this vedio. .

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You are doing great ❤

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Excellent💯💯💯

[@satishkumarsingh3919]

Excellent lecture.

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[@saikumartallaparthi8090]

Tq lot of learning ponits for hplc Ur video

[@shribhagwan4539]

Thanks for sharing valuable information on different aspects of HPLC

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[@mydia125]

Thank mam for presenting such knowledge for chromatography..

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[@pharmasolutionpakistan7668]

Good job i am a teacher as well as lab instrument trainer i appreciate yu your convying mthd z very good

[@prakashg7066]

Thank u so much and ur explaining style is beautiful & easy to learn!

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[@manishbhoye4344]

Nice lecture on different aspects of HPLC method development.

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[@indie1751979]

Very nice lecture on HPLC. Thank you.

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[@zahidakhan1304]

Very well explained...thank you ma'am 👍

[@aniksinha3734]

Excellent presentation of different aspects of HPLC, Thank you mam.

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Thank you

[@BARATHKUMARK-cp7ll]

Thanks mam You lecture was very useful for me

[@dr.nayanpanchani9015]

Very good explanation on method Development of HPLC

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[@gaganrathore5455]

Thankyou ma'am, for HPLC method development explain in easy way 😊

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[@manukumarbhandoria2554]

Thanks a lot mam for the detailed presentation in such a lucid way.

[@samirkotkar1513]

Sanket want to talk same things

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Helpful information 👍🏻👍🏻👍🏻👍🏻

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[@06_soniyakundnani13]

Thanks for nice explanation mam its really helpful

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Thanks mam It was very fruitful for me

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[@bharatmahajan1753]

Good video for team thank you

[@vimalthakur1602]

Very nice knowledge full points👍

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Very thankful madam 💓💓💓💓

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Superb mam, 🙏

[@chandrasekharm8628]

Very good information and expline medam

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Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.

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Fantastic

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Nice video. Keep it up👍🏻

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Outstanding presentation 👏

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excellent lecturing madam

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VERY INFORMATIVE

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Great lecture.keep it up

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[@monashah4079]

Excellent explanation of HPLC

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[@madhurahegde2603]

good information...

[@PharmaceuticalConcepts_Ojaswi]

Excellent madam.. Very nicely explained..

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[@vinayakkumbhar725]

Nicely explained and very useful beginners

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Explanation is best.

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[@renganayakidharmarajan9257]

excellent presentation

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Many thanks

[@janmahho1547]

Thank you very much for your efforts and do you have any information about validation HPLC?

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Excellent PPT and excellent explanation

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[@sahidulchem]

Excellent lecture mam

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Very very excellent presentation thanks mam

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Salute to mam💐💐

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Totally Good 😃😊

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Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)

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Excellent lecture

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[@dr.devangr.pandya2169]

Very excellent

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[@Juniasheryl]

Good lecture

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Excellent

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[@dr.s.rajalakshmi5424]

Nice video

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[@santidas1]

nice session on HPLC method development

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[@dorakondaanilkumar6044]

Thank you madam... good explanation...

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Thank you

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Very good

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[@eswarbabu6938]

Excellent lecture madam,,,, thanks🙏..........

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[@ramnandaniya7636]

Interesting lecture

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[@isaks1880]

Very nice mam

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[@92dahman63]

very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)

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Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC) Insil Kim,1 Sean A. McKenna,1 2007

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Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.

[@mangammollasrinivas6003]

Madam please du more videos regarding method development... What are the common problems we are facing while performing method development...

[@kirankaarumanchi]

Your explaining very nice madam

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[@rainbowchemistrysudhakar4913]

Super explanation

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Thank you so much mam

[@krish_krish354]

How do we know that from structure our compound is ionizabale and non ionizabale

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Generally salts of acids and bases are ionizable in nature

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[@krish_krish354]

36 minutes chiral detector ion detector comes under which property detector bulk or solute

[@krish_krish354]

What is the use of retention factor and gradient retention factor

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In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption

[@HariKrishna.vallepu]

Excellent mam. Can you explain GC

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Good

[@shailendrakushwaha7266]

Thanks ma'am

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Most welcome 😊

[@krish_krish354]

Can you explain what is column back pressure

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Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.

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Column back pressure increases in short or long column17.48 min

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with longer column , column back pressure increases

[@MEMEMAKER-l6q]

M pharm me rp hplc method development kese karna he vedio plzzz

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This video only

[@MEMEMAKER-l6q]

@analyticaltechniques7088 mam how to operates full hplc for m pharm projrct rphplc any suggestion plzzz as our college dont have hplc and they are teeling to do cousre which is cost of 6k and we cannot afford it so how can i learn free hplc for project

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