Preparing Tris Buffer

[@ZulhilmiHusni]

Did you know that TRIS will form precipitate with Ag+ in conventional pH meter electrode? One does not simply measure TRIS buffer in pH meter with Ag/AgCl electrode with single junction reference electrode (mostly used in conventional pH meter now days) because it will poisons the reference electrode. using calomel electrode or double junction reference electrode is the correct way it seems that your using single junction electrode (i can see it clearly) and therefore your actual pH in not 7.8.

[@DocScoop7]

no calculations mentioned, how do we know we need to add 15.14g of powder? where does it come from

[@mariodanielrodriguezmaravi6741]

If you know basic chem, it is just stoichiometry

[@nathancoleman8648]

Great video, very helpful, but I think your N on the NaOH bottle is backwards.

[@onnorokom2023]

The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer

[@aminaferielkadri1248]

can you make a video about how to prepare lysis buffer

[@quranicversesrecitation1]

Why we only use HCl and not any other acid for stabilizing ph please answer

[@dmadhav4867]

how can we know to add 15.4g of tris buffer in sample water

[@bbmeesci5950]

I had no idea that you could add HCl to your buffer to alter the PH. my mind is blown.

[@sasidharankr2344]

Yes you r rite..it should not be done so

[@WeezyGR]

this is the correct way, what do you suggest​@@sasidharankr2344

[@coca833]

I have a question, if I want to prepare a solution at a concentration of 100 mM pH: 7.8 from this solution(0.5M, pH:7.8), I have to re-measure your pH? Thanks for you video.

[@XxXnonameAsDXxX]

When i add the tris my solution becomes cloudy. I have to adjust the pH with hcl. Only after it will be clear again. I'm using 1 g/l of tris(THAM). Is this normal?

[@larissabts6638]

So amazing 💗💗💗

[@wardahmustahsan888]

can you do a video on CTAB buffer?

[@monsabas5771]

good video ,,thanks alot,,, but can you help me about the best buffer that can be used in my experiment when my drug is unstable at phosphate buffer,,,, means that when i prepared the phosphate buffer the uv absorbance was not stable that changed with time but i need the same absorbance for 14 days !!!! so what the solution ?? plz help me :((

[@erygusman4557]

Thank you for the Video, it's really helpful. What we put instead of HCl to increase our pH Buffer solution?, for example if my buffer pH is 8,5 but I want it pH at 9.0

[@meghnathapa2273]

NaOH pellets

[@NOURALB_24]

Why we didnt use boric acid?

[@medineadin4700]

Thank you it helped me a lot !

[@khuntalhashah6433]

can you please upload some video on kreb henseleit buffer?

[@maryambibiabdullatifzargar6521]

how to prepare 10mM tris buffer needed for DNA extraction??

[@muhammadfarazali4251]

Which powder u have used?

[@nahlaalshaikh581]

Good video

[@kailed]

does it work with amylase?

[@ryanpersaud95]

Very helpful thank you

[@bhupendrabhatta5850]

What is the temperature and time for autoclaving?

[@msr9452]

Great 👍

[@HamburgoYT]

Aren't ph meter bars aren't as accurate as ph paper?

[@joeriv5462]

You switched it. pH paper is not as accurate as pH meters

[@HamburgoYT]

joeriv5462 That's weird?!! In our biotech lab they prefer us using ph paper and said it was accurate. I think its because its faster...

[@joeriv5462]

Hamburgo pH paper can be faster since all you have to do is insert it into your solution and look for color change. pH meters are quantitative so you can determine the exact pH of your solution.

[@HamburgoYT]

joeriv5462 Sweet! Really appreciate it :D

[@babubassa8434]

No. They cannot be.

[@maryambibiabdullatifzargar6521]

great

[@darklight1030]

what!? I dont even know if I should autoclave or filter!

[@brnkaik]

me neither

[@deryacelik7743]

tnks to video

[@mariadeassis5484]

Olá...muito legal.

[@TheAquaventures]

0:58 do NOT stick your scoopula directly into a reagent bottle!

[@leewilliam3417]

Mmmmnn😊

[@martinez3499]

I'm spiderman