primary animal cell culture | Disaggregation of animal tissue by enzymatic and physical method

primary animal cell culture technique | primary animal cell culture in Hindi | disagregation of the animal tissue in animal biotechnology or animal cell culture | physical and enzymatic disagglegation method animal cell culture or animal biotechnology
this video is helpful for the student of BSC,MSC zoology,botany and Biotechnology student.
animal tissue culture is a important technique of animal biotechnology and zoology. In the animal tissue culture cell is isolated from the animal cell and then culture in culture media . in this case most typical think is isolation of the cell from tissue. for the isolation of the cell from tissue use two types of method in animal biotechnology.
1. physical disaggregation of animal tissue in animal cell culture.
2. enzymatic method in this method used two main type of enzyme for the disation of the animal tissue in animal biotechnology
a. disagregation of the animal tissue with the help of collagenase enzymatic method
b. designation of the animal tissue with the help of trypsin in animal biotechnology or animal cell culture it is two type cold trypsinization and warm trypsinization.