Primary Cell culture and cell line | Cell culture basics

[@bhaskarsaha8296]

Hi Arpan, Excellent video!!!! I am going to use it for my MSc students. Great to talk you you last week and to know about your channel.

[@animatedbiologywitharpan]

Thanks Bhaskar da, all the presentations are available in case you need let me know

[@park_chaeni]

wow....Your lecture is perfect. Thank you so much! (from KOREA)

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Korea 2

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North or South

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@ of course, South

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No explanation in the world can be better than this!!!!

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Omgggg!!!! The real images of hippocampal primary culture are crazyyyyyy!!!!

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Good to hear from you ....stay tuned and keep watching

[@ikfacts3604]

outstanding video and such an informative lecture of cell culture. thanks

[@umm2825]

Let me tell you this is the best video i have ever seen. I mean you are literally so great. The way i understand ufffff. Thank you so much

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[@ma_kal]

Thank you for making this easier. Keep up the good job.

[@alondragonzalez3662]

I LOOVE your animations and the way you explain all the techniques. Very useful for us master or PhD students! Thanks for sharing this content.

[@animatedbiologywitharpan]

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[@driti8310]

Really thank you so much for this video..i was reading a paper about immortalized astroglial culture and wasnt able to understand properly...ur video literally gave me a clear base line...thnks once again

[@animatedbiologywitharpan]

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[@arijitdas8569]

I hereby appreciate the way you explained animal cell culturing, and especially hippocampal culturing and widely used organotypic culturing. Thank you for this informative lecture! I've loved it. :)

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[@Pet-friends6379]

your video is well organized...explained a lot...thank u very much...

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[@deepikamks]

Thankyou so much again...me being a m.sc in chemistry...now doing a phd in chemical biology ...i need to know these basic terms. Reading does help but animated videos are just so awesome. And your videos help me a lot.

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@@animatedbiologywitharpan sure. ...im yet to explore other videos from your channel. If i dont find them, i would request you to make a video on them 😂

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Please feel free to suggest

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@@animatedbiologywitharpan like RNA modifications and their role in antibiotic resistance as well as cancer, phage resistance and ways in which viruses evolve to finish that resistance. 😇

[@katherinensumfor1374]

Nice video, I do have a question, you said before that cell lines have limited life span and then on the table it says cell lines have unlimited lifespan, is there an error somewhere?

[@branman399]

you say at 3:30 that cells with highest capacity of growth predominate - does that mean as you create more subcultures, you will get more and more of a single cell type (being the cell type with highest growth rate)?

[@goddessoffun4763]

Best video so far

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Brilliant video! I love the animation

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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@ozlemkalkan5167]

thanks for this helpful video :)) It seems a crazy future awaits us

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[@huxsa]

I really like this video! Nice drawings!! 🥰🤗 Greetings from Germany ☺️

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[@vaishali7538]

Can we get video on techniques used for single-cell culture?

[@travellingkorea]

very easily elaborated contant for basic understanding

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[@shashios1236]

Very nice nd unique.... thanks

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[@byul8100]

thank you arpan, it helped me alot :!! can't wait for ur next video

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[@J25kwt]

Thanks a lot for the beautiful video. you help me in doing my lab report. 😁👏🏻👏🏻♥️

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[@shroqhafed5717]

wooow,Thank you for making it easy

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Thank you so much 🙏🙏❤️ Appreciate your efforts 💯

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[@omshrisingh1101]

You are really pro 🙏

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[@wannabee525]

This was so great! Thank you so much!

[@jaydebpal1818]

hello arpan , your videos are really good. can you please suggest me some good but simple books for cell culture

[@animatedbiologywitharpan]

You can reffer to Wilson and Walker book but honestly its a practical thing so we learn it once we do it

[@vanithavippala2104]

Very good explanation sir🥰👌

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[@donnaandre7633]

Excellent!

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Live savior indeed thanks

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[@lugienal-khalaileh2061]

if we make cultured for endothelial cells these cells may not accurately reflect organ specific endothelial cell characteristics what is the reason ? ist related to aberrant in gene expression or what happen ?

[@animatedbiologywitharpan]

Very nice question , in reality extracellular matrix plays important role 8n tissue morphogenesis and cell fate specification . If we use isolated cultures, all those external signaling will be absent and hence fate specification could be altered . It's always a problem for cultures

[@lugienal-khalaileh2061]

@@animatedbiologywitharpan Thank you very much

[@sakshimehra8199]

sir i have a question that a normal cell line will die after how many division?

[@animatedbiologywitharpan]

Generally after 7-10 passage it should not be used for experiments....cells might accumulate many mutations and undergo telomere shortening...but this is highly variable for different cell lines

[@ahau221]

How can I make that media n serum for vito meat. U have the video sir?

[@animatedbiologywitharpan]

Sorry bro 8 Don't have a video on that

[@vtc011]

Thank you sir!

[@T_44a]

just to confirm, following the passaging of the cell and centrifuge for cell suspension, the cell is then left in the media to grow?

[@animatedbiologywitharpan]

Ya very little amount of cell from the suspension is transferred in a new plate containing media for further growth

[@animikhaghosh6536]

Can you explain me the 8:17

[@mohamedkhan7560]

Thank you!

[@animatedbiologywitharpan]

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[@logeshwarana.r4461]

Sir can u refer books to learn cell culture techniques

[@animatedbiologywitharpan]

Honestly there is no good book for learning cell culture....you have to hit the lab to learn about it

[@safwankhawja]

Zabardast 👍

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Bro I appreciate the effort your lectures are very organized pretty and well done. Also, you’re good in explaining complex ideas or subject in such simple way. Thank u so much for everything you’re a hero to all of us.

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great work

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Thanks Namrata for your compliments

[@SaroSalh-r6g]

When cells are separated by trypsin

[@ramyakiran2349]

Hi..this vedio helped me a lot .. Can u pls let me know where can i buy head and neck cancer cell lines (specially tongue) in india at reasonable price

[@animatedbiologywitharpan]

I don't know it

[@rashikasingh7607]

Hi , is it possible to produce whole liver from a single liver(hepatic)cell ?

[@animatedbiologywitharpan]

Yes now a days scientists have developed liver organoids ( mini liver ) check it out www.frontiersin.org/articles/10.3389/fmed.2021.574047/full

[@rashikasingh7607]

@@animatedbiologywitharpan thank you for providing the link ,I just read the Article in which they using genome editing, tissue engineering technology,chip based technology and after reading the article I am more keen to study more about these technologies ,thanks!

[@animatedbiologywitharpan]

@@rashikasingh7607 I work on same technology….but I build brains…feel free to reach out at [e-mail]

[@rashikasingh7607]

@@animatedbiologywitharpan nice,I am a MSc.biotech student I also wanna do some interesting research or build some helpful techniques for us ,will definately do.. thanks for motivation.

[@animatedbiologywitharpan]

Good to hear from you….stay tuned

[@kgkgmhmjl5240]

Primary cell the same with primary cell culture?

[@animatedbiologywitharpan]

Yes

[@romanperez2814]

Can you share us the bibliography please? 🤗 "Saludos" from Mexico

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Please send me an email at [e-mail]

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[@romanperez2814]

@@animatedbiologywitharpan of course, thanks a lot.

[@navyamaryjohn5732]

How are cell lines immortalized??

[@animatedbiologywitharpan]

Immortalized cell lines are derived from a variety of sources that have chromosomal abnormalities or mutations that permit them to continually divide, such as tumors. Because immortalized cells continuously divide, they eventually fill up the dish or flask in which they are growing.

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Thanku sir for this video

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what is mean by cyhrid

[@animatedbiologywitharpan]

I will give the answer in a different video

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@@animatedbiologywitharpan okay

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🙏🙏🙏

[@animatedbiologywitharpan]

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Tees aren't available in India?

[@animatedbiologywitharpan]

It would be available soon via vistaprint....vistaprint has offered a sponsorship ...we would bring these merchandise at very reasonable cost ( bellow 500)

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nice video

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What is PBS solution

[@animatedbiologywitharpan]

Phosphate-buffered saline (PBS)

[@kaoutarkawthar4442]

Slt Svp esq vous pouvez m'envoyer le protocole de la culture cellulaire des hépatocyes!!?

[@sabah.bsabah.b1559]

what mean PBS

[@shanmugapriyavarshini6761]

Phosphate buffer saline

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[@animatedbiologywitharpan]

make sure to checkout entire cell culture playlist kzbin.info/aero/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2&si=wryecbGL3FEk4uon

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can u share notes?

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It's chargeable $5

[@NhungNguyen-yu5hs]

the sound is not good I am quite disappointed

[@animatedbiologywitharpan]

Sorry about the sound...

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👍👍👍👍

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I'm sorry but I just cannot get through the language barrier. While I'm trying to comprehend one sentence, I'm missing part of the next, and so on! I see by others' comments, they could...I'm happy for them...

[@animatedbiologywitharpan]

I am so sorry to hear that

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NEET

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That fake accent though😂😂