Pulsed Field Gel Electrophoresis (pfge)

[@abmalshehry1]

You should be named "Molecular Genius" aka "Molecular Genetics". I wish some of my PhD lecturers at my MSc course would watch your videos and learn how to simply and effectively teach this Unit.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@abmalshehry1]

@@shomusbiologyofficial and so nice and thoughtful as you reply to every single comment. what a gentleman.

[@swetayadav28_13]

7 years ago video yet the clarity !!...👏🔥💯

[@shomusbiologyofficial]

All the best

[@Diogenesthedog0]

The OG biotechnology instructor.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@kewinmalla6233]

shomu biology is an amazingly reliable tutorial site,i can find all my syllabus here. Thanks from NEPAL.

[@shomusbiologyofficial]

+Kewin Malla great. I am so glad to hear that

[@kashafseducationalsite363]

i dont knw why whenever i need to clear my concepts i prefer u.. u r amazing sir ..

[@shomusbiologyofficial]

Glad you liked my lectures

[@samuelforward8453]

Thanks from a Food Science student in the UK!

[@NisthaShukla-l1v]

sir your videos are really helpfull to understand techniqes and method👍

[@shomusbiologyofficial]

You're welcome

[@akhilajayamon3432]

All ur videos are really helpfull for my studies, and thank u for that. U are blessed with a good teaching skill. A good way of communication and convenient explanation. The main thing its very easy to follow u sir. Really hats off. Videos regarding microscopy, chromatography and electrophoresis are awesome. Once again appreciating ur effort ❤️

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@강아지-m5j]

It was very helpful! Thank you and your lecture style including your pronounce style is really catchy. It really helped my understanding.

[@swetaxavier6290]

Thanks for the video. U always rock

[@shomusbiologyofficial]

You're welcome

[@vaishnavikhavate5092]

It was very helpful & simply amazing.tq sm for your lecture.

[@kalyanlohar476]

Thank you Sir... Thank you 👍👍👌💞💞🙂🙂🤗

[@shomusbiologyofficial]

You're welcome

[@BaybeeDoll15]

thank you! very helpful when you explain it step by step :)

[@shamsarefin5680]

Ton's of thanks

[@shomusbiologyofficial]

Thank you

[@dipti7604]

Just loved it ....very helpful vedio . Thank you soooo much.

[@amnasarwar39]

Help alot. Kindly make video on microchip electrophoresis and concepts related with it.

[@satabdeepanda6810]

Thank uuu so much sir.😊

[@shomusbiologyofficial]

You're welcome

[@lidyahandayani4744]

Very nice video... 1 question : why do we need to use a gel (plug) to digest DNA using restriction enzyme? Why dont we just put a DNA suspension + restriction enzyme, and then put them in wells and run in pulsed field gel electrophoresis?

[@jyotikashyap5368]

Lidya Handayani maybe to avoid the step invovling isolation of the entire genomic dna of the bacteria,will save time.

[@heartforall5853]

Lidya Handayani I was wondering the same, but I think maybe because a normal DNA extraction will sheer/damadge the DNA a little bit and the agarose is maybe to protect the DNA and let it move easier in the agarose gel?

[@pushpasharma1813]

Because during the extraction process some of the DNA fragments might get degraded . So to avoid this , we use agarose plugs.

[@fatemehakbari2189]

thank you . it was really very useful just like always .

[@harryporterfield5701]

I recognize one of your images as coming from the CDC's PulseNet website. Where are the other images from? Please cite your references.

[@abhilashasharma6255]

VERY WELL EXPLAINED

[@niveditharajendran5113]

Pls do a video on isotachophoresis

[@EducateWomenEmpowerWomen]

Sir, is agarose gel electrophoresis also known as submerged DNA electrophoresis? Or it is another technique?

[@akvaryumdunyas5594]

Thanks, very helpful

[@anacarolinaprado1521]

Hello, I am standardizing PFGE for some kind of fungus and my races are getting blurry, with no definite bands. I wish I could have your contact and exchange ideas about what I could change in my protocol.

[@bhargavireddy5730]

Sir.. I have a doubt.. When we change the direction of the current there is a chance for the DNA fragments to get migrated into the adjacent lanes? Dnt they mess up?

[@shomusbiologyofficial]

They never travel through straight lines. And pulse field gel is kind of messy

[@bhargavireddy5730]

@@shomusbiologyofficial so.. Ecah time we will run only one sample sir?

[@MeenuBagariya]

Nice👌👌👌👌✍️✍️✍️✍️✍️🙏

[@shomusbiologyofficial]

Thank you

[@heartforall5853]

what about the other cell components? will these still be visible in the gel?

[@shomusbiologyofficial]

+Shinju Aishiteru no. For different types of cell components, we need to run different types of gel

[@Greanestbean]

Thanks!

[@shomusbiologyofficial]

+Jon Roe you're welcome

[@uar9596]

Why there’s no audio?

[@AmarSingh-ms1kg]

Audio is audible from one side while using earphone.

[@kalyanlohar476]

thanks Sir.

[@samrahahsan2337]

there is no voice in video why its so

[@Realpeople90]

nice one

[@vivekabhyankar5029]

good1

[@abhishekrana5933]

board pe jyada smjh ata hai

[@shomusbiologyofficial]

Board video on offer coming soon