PYQ 4 GATE Biotechnology (BT) 2018

#gatebiotechnology #gatelifesciences #csirnetlifescience #enzymekinetics #MichaelisMentenkinetics #LineweaverBurkplot #Km #Vmax
I have discussed the Lineweaver-Burk plot when a competitive inhibitor is present. In what way does a competitive inhibitor raise an enzyme's Km value? And how a (very) high concentration of substrate leaves Vmax unaltered? Concept of apparent Km (alpha Km) in presence of a competitive inhibitor. Definition of dissociation constant of enzyme-inhibitor complex.