Regulation of transcription | Biomolecules | MCAT | Khan Academy

[@ratatat12356]

You should of clarified that operators are not present in eukaryotes, only in prokaryotes.

[@CCastroA17]

this is not true. There are lots of articles even from 1990 disproving this point. However they where thought as uncommon, they are just as diverse as in prokaryotes, often being misidentified with transposable elements.

[@rundown3984]

@@CCastroA17 i think he means in the ap bio curriculum

[@googoothedoo]

"should have", not "should of"

[@Bayooneh1]

She speaks way too fast and doesn't write enough, had to pause a thousand times. Also, you she didnt mention that Eukaryotes DO NOT have an operator like prokaryotes do...and didn't mention a LOT of necessary details about transcription. Sal is so much easier to follow...please slow down and take more notes in future videos.

[@dARKf3n1Xx]

biology bro, verbose explanation. she can't condense it else it might change the meaning

[@lalaland1454]

I was confused when I saw the operator there. I was starting to doubt my Molecular Biology professor.

[@rundown3984]

Thanks for clarifying this my man eukaryotic transcription and prokaryotic transcription are TWO different things

[@winsavierelias]

Okay umm l get it but some people prefer it this way just try finding someone for you COZ ppl work differently and others like it like this

[@qwer-pg3hv]

Thank you for that comment! First of all the Eukaryotes have many different transcription factors and the Complex of the RNA Pol2 has subunits, thus names are very important. And about the fast speaking bro this is science you dont find a chilled video hahaha

[@khushanrahanu4959]

In the beginning , the given examples of the cells (i.e., sensory cells and Gut cells) are actually examples of eukaryotic type, but then the actual topic is based is found to be based on prokaryotic cells (i.e, in Escherichia coli).

[@vladyslavlazebnyk5674]

exactly!

[@lakep7798]

Yes!

[@mnoori100]

Why are you mixing Prokaryotic and Eukaryotic gene expression?

[@alimesemenli59]

+Bella yes. enhancers and silencers are found in eukaryotes while operators are found only in prokaryotes. It was kind of confusing to mix these up.

[@nefo706]

she speaks like a motor boat i had to pause million times

[@xma3559]

Exactly! I couldn't even follow in the first few minutes.

[@hazka58]

Like a motor boat? She speaks perfectly fine... maybe your brains are just slow :P

[@lavenderrose2606]

بشر تخرجت ولا بعد😰😰😰😰

[@nefo706]

Lavender Rose ههههه لا ابشرك الحمدلله تخرجنا زمان وصرنا اصحاب عمل😂

[@lavenderrose2606]

nawaf faris ماشاء الله😰😰😂😂😂 عقبالي😭😭

[@Rosiewilding83]

I agree, I do love Khan academy for its ability to break down complicated information into understandable content but unfortunately this girl is just too fast at delivering a muti step process.

[@MsCassiexo]

The main guy explains things better- he's so much easier to listen to!!!! :(

[@techshiamitchelljohnson1215]

I totally agree. I am not one to tear anybody down but this is not working for me. So sorry Khan.

[@flow4458]

@Mike Kane THANK YOU!

[@chinyere95]

Hey good video, but I think upstream is 5' and downstream is 3'. I noticed u mixed it up a bit

[@jjepistle4934]

chinyere95 if the strand she labeled is template strand i didnt see anything wrong..she just didnt draw it in the conventional way

[@sam-ut4ib]

chini you'r right..

[@sunkenarrow8390]

The label is actually correct, in the fact that she labelled the strand as the antisense strand which runs in the 3-5 direction. She labeled it this way because dna is synthesized in the 5-3 direction and this strend is being used as the template.

[@ss-nu8xx]

You are wrong not she

[@zainabahmed6335]

I am not sure if there was a time limit to this video but it felt like there was a fast forward button in this. As a visual person, I was getting anxious having to replay the same sentence several times even when I stopped taking notes to just absorb what's going on. I would appreciate slowing down and putting up pictures even if they are not drawings

[@chita4lyfe]

Activators don't bind to the promoter, only the basal transcription factors bind. Activators bind to enhancers, silencers, or promoter-proximal elements.

[@jimwelbryanchristopherferr3208]

I don't think so. Some activators bind inside promoters. Basal transcription factors recruit RNA polymerase. Activators do not and have other ways of enhancing transcription than merely recruit RNA pol.

[@zahraoasti106]

my god, had to play this one in slow-motion.

[@MyLady22]

Thanks for confusing me even worst

[@avelinoardhika4165]

OMG how many lightyear does her speaking go?

[@modolief]

This is the most molecular biology I've seen compressed into a short time sequence. Any more data and it would create an informational singularity with an event horizon! Honestly though, I'm impressed. Very interesting, stimulating content.

[@88KhanOmar]

Great video, but i feel you spoke very fast, maybe perhaps you had limited time to make the video. I literally wrote every single word she said a 7min video took me like 35min to cover all the martial, but other then that great video!

[@Canada2760]

Prokaryotic and Eukaryotic transcription shouldn't be amalgamated into one explanation. They are very different in many ways.

[@neeshantn4701]

What a voice ...

[@tahsinuddin]

ngl this is the first time a khan academy video has left me more confused...

[@hollyberi6695]

Is this for Euks or Proks? This video is not as good as the others, she speaks too quickly and it's not as well-explained.

[@ss-nu8xx]

For all of you who have been saying that she messed up upstream and downstream - she said 3’ end of the ANTISENSE strand (the template for mRNA) is upstream and that is true. You have memorized like a parrot that 5’ is upstream without understanding which strand (template or mRNA) is being talked about and the antiparallel nature of the strands

[@tylerjohnson704]

I have no idea what's going on; get Sal on the line.

[@huzzahpringles9011]

you didn't explain enhancers at all. just listed some components and said they loop the DNA "so that you can then recruit RNA polymerase and transcription factors"... which are recruited anyway...?

[@georgia614]

i like ur hand writing. thanks for the video!!!!!!!!!!!!!!

[@tarungupta1245]

speak slowly

[@nadiacharafi8815]

Gosh! you mixing everything up and confusing us more

[@legenarymaster3696]

I think you put the location of the enhancer and silencer in the wrong spot. Pretty sure it's supposed to be upstream of everything, not downstream.

[@manotialawadi7085]

It is very high level

[@CaptivaLP]

Is pretty misleading. You explained the procaryotic regulation but took eukaryotic cells as example

[@samuelrestrepo4489]

too many small mistakes regarding the promoter, the enhancer, where they bind, etc. It's obvious that she knows her stuff but a quick review before teaching would not hurt.

[@samudrakalita1361]

Plz, write the subtitles also which makes more easy to understand if u can.kindly act on it

[@thevanilladoll]

I had a hard time following. I had to slow down the speed to 0.5 so I won't get lost.

[@dayeongE]

thanks for the upload but isn't operator rarely in eukaryotes? And isn't operator to eukaryotes is promoter-proximal element..?

[@chita4lyfe]

No both have operators and promoters

[@bodybuilder6350]

Is this lady reading from a script for Guinness world records?

[@Movewithkhu]

Which gene is transcribed n which not

[@yourfuturedocburenbeiya]

Great video, but I am now more confused where upstream is. I thought it was on the 5' end. When does the upstream go on/towards the 3' end? Thank you!

[@cdorman11]

5' end of the RNA, 3' end of the DNA template strand

[@luqmansiddique8591]

2 years late, but basically in DNA transcription, the template strand is used for the actual transcribing while its antiparallel strand (the one it was attached to, called the coding strand) is used for referencing the direction. So when 5' is usually upstream, when talking about the template strand, we call its 3' end upstream, as we are actually referencing the coding strand.

[@miaeba]

upstream is that correct way

[@ElloBoppit]

To parrot what everyone else was put off about, need to be clear about eukaryotic/prokaryotic systems. This was a bit confusing.

[@luckygreen9061]

Shouldn't have started the video by introducing eukaryotic cells and bringing up operators since they really aren't common

[@kristenarce1618]

I think she had some stuff confused.... upstream is 5' and activators don't bind to promoters.... they bind to enhancers and silencers. Operator is also in prokaryotes and not in eukaryotes

[@cdorman11]

Upstream is closer to the 3' end of the template DNA strand, since the RNA polymerase attaches 5' end of nucleotide to 3' end of pre-mRNA strand.

[@luqmansiddique8591]

Basically in DNA transcription, the template strand is used for the actual transcribing while its antiparallel strand (the one it was attached to, called the coding strand) is used for referencing the direction. So when 5' is usually upstream, when talking about the template strand, we call its 3' end upstream, as we are actually referencing the coding strand.

[@litojonny]

everyone is complaining about how she's talking too fast while i'm watching this in 2x 8D

[@juandiegogiraldo7368]

too fast slow down, I say fireddddd

[@YousefSoltan1990]

Isn't it 5'-3' ? 😶

[@imagination7710]

+RAF She said 3' on the *antisense* strand, which makes more sense to describe when your talking about elements which bind to the antisense strand. I actually don't understand why the convention is to designate 'upstream' as the 5' region of the sense strand.

[@josam4545]

C'mon give the nice lady a break. No need to compare her with Sal. I think if you're gonna constructively critic her presentation then just do that, but its not nice to compare.

[@sohailalikhan2562]

Shut the fuck up, you sound like the last nigga I know

[@josam4545]

Must've been a nice one.

[@sanji296]

DA! YOU STAYYY HEEERE!!

[@Tahassaleem]

slow down plz

[@Tahassaleem]

FWRRGWR

[@Tahassaleem]

GSGDFGG

[@Tahassaleem]

GSDFGDGSG

[@chechennyboiiy]

she has a nice voice :)

[@JRockTT]

There is so much wrong with this video. Honestly, I do not know where to begin.

[@sciencenerd7639]

hmm, aren't enhancers usually upstream, not downstream?

[@Ice84letters]

they can be in both places

[@mistrotman420]

3'&5' should be switched

[@yarelimaciel2149]

Good I am not the only one that thinks this.

[@thelastcipher9135]

yada yada yada yada

[@3rdpartyU5er]

Nice lady please, DRAW A BIGGER PICTURE of things you are explaining. Look what Sal does.

[@koondoog4282]

isn't upstream is towards the 5' end?

[@TravelGal75]

the upstream is labled wrong upstream is in the 5 prime direction

[@jjepistle4934]

STEPHANIE ASCHER if the strand she labeled is template strand i didnt see anything wrong..she just didnt draw it in the conventional way

[@JesusCruz-tb7sm]

Don't watch the video- very confusing.

[@نورة-ذ1ن4د]

please translate to arabic :(

[@rosepearl7092]

Hey, are you teaching or showing off?

[@noorpk]

Many mistakes. Not recommended

[@emptypotatoskin]

She doesn't explain these concepts well.

[@qwer-pg3hv]

Please clarify that the operater are not present in eukaryotes very irritating when you start to study this subject!

[@norainiazhar5298]

she's speaking too fast i just couldnt catch up.

[@marrocos-leonardo4816]

Confusing

[@melissahoaglund5196]

Too basic. This channel is usually better than this.

[@maxanstine1139]

This was definitely way too fast. I would rather everything be well explained and have the video be twice as long.

[@stuartkelly3106]

I find voice is too hard to understand as vocal fry is too strong.

[@stuartkelly3106]

Her vocal fry is just too much for me,

[@AnastasiaLaurel]

TOOOO FAST.

[@Alien_hunter.]

heeeey

[@МартинЦеригг]

Not as good as other videos. She is not that good.. Alot of pauses even though I kinda know my lessons..

[@David-sj4fk]

Waaaaay to fast. Way to many things at once. Way to many new words without any real explanation. Makes it hard to focus and where ones focus should be. Sorry. but a downvote from me, I learned basically nothing.

[@poojachaudhari1331]

you are going too fast . I like sal better

[@naz_323]

I couldn't focus because she speaks way too fast

[@franklinw2485]

I absolutely agree with you. I think this lady was showing off.

[@syefferdaus5677]

for lecturers, this is the worst kind! couldn't follow+she mixes up procaryotic & eucaryotic cells!!

[@AJAL9574]

talk fast , got me lost Dislike

[@mrfrost6337]

very badly explained

[@쩡지니]

had to study this and oncogene but i couldnt get the help from khan academy this time because sal wasnt explaing it for me. i dont want to be rude but i dont think teaching is the thing for you. you make things sound harder than it seems and you cant really make people understand it, you just pour out the information and make people teach themselves like my professor.. i was utterly disappointed and i wish sal comes back to redo this concept.. im sorry but please don’t make any videos from now on. i wish to see sal on them videos, thanks

[@idreceghadie]

way too fast