Restriction Digestion of DNA

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This video demonstrates how to set up a restriction digest using the Bio-Rad Restriction Digestion and Analysis of Lambda DNA kit. Techniques for setting up a restriction digest using a micropipet are shown.
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[@beanheadr]

Interesting that this process has not changed much over the years. I did a high school internship at the Kennedy Krieger Institute back in the late 90's working with X-ALD with mice as our catalyst. I remember being nervous using ethidium bromide after my lab director explained that exposure to it could cause my DNA to mutate!

[@arseniipavlenko1048]

So no one is gonna mention that the tubes spell out HELP at the end?🤔

[@neil1988]

HAHAHA 🤣

[@esther9223]

First thing I noticed🤣

[@geneie6050]

4ul lambda DNA 5 ul buffer (EPH) 1ul restriction enzyme Centrifuge, incubate overnight, run gel

[@arresteddevelopment2158]

Will I ever get to do this again? It was fun. Sigh

[@nbent4607]

Really helpful, thanks a lot!!

[@angeliebacon8276]

never seen green equipment 😍

[@siddharthsrivastava8652]

Video on calculations that go into setting up such restriction digest kzbin.info/www/bejne/m6XLaoCea7Omq5Y

[@saranyasakthivel5071]

it is very useful to understand

[@Han-cp9ds]

what do u mean by corresponding tubes for the different restriction enzymes? this video was informational!

[@Han-cp9ds]

ok thanks!

[@jinoralphdarcega174]

@@Han-cp9ds You're Welcome

[@garimaupadhyay3469]

whats the name of the buffer???

[@rajubharti8881]

Is restriction enzyme added in L tube or only in E, P, and H tube ? make it clear .

[@marijatrajkovic3320]

To each tube

[@hardikvora788]

Maybe L is a control

[@GooglyGiggle]

Pls add subtitles

[@RobertJenkins-h6p]

Buckridge Brooks

[@jiminslonglostgrapejams2582]

Bts dna

[@Iloveshymenfr]

Army..?

[@doreamon1248]

Bts army love from India.....

[@student_remo]

Jungkook, molecular biologist