Extracting Plasmid DNA: How To Do a Miniprep

In this method video, Molly takes us into the lab to teach us how to purify plasmid DNA from a liquid culture of bacterial cells. When purifying plasmid DNA from a small volume (3 to 5 ml) of bacterial cells as we do in this video, this technique is called a miniprep.
View this video (and more like it) on LabXchange: www.labxchange.org/

[@bullymag]

This work deserves way more than that amount of view. Excellent work. Liked and subscribed!

[@samsonkamau5048]

I am not used to commenting but this one deserves a thumb's up. excellent explanation with clear and precise illustrations

[@user-zd7xv7wc2t]

This video is one of the best teaching for learning extraction plasmid.

[@birdo54]

This is a FANTASTIC video! Thank you very much, this was extremely well done.

[@LabXchange]

Glad you enjoyed it!

[@shirinbehnamsani2366]

The first time I understood what I was doing this whole time in the lab. Thanks for the amazing explanation!

[@gapat8213]

What an incredible science humans has developed. This is something to be grateful for everyday. Plasmid will have the potential to cure all diseases when the special interests will allow it.

[@christopherleubner6633]

Very informative. Highly recommended for molecular bio and genetics students. ❤ ill take my plasmids chilled served neat. 😂

[@mateuspaiva5916]

The material in the video is amazing, and the scientist is also an amazing actress! Great job in here, keep up with your inspiring work.

[@pingsart7402]

wonderful demo

[@isaacco2876]

this is a great video! thanks for the info!

[@KatayonZare-uq9cm]

That was PERFECT thank you for your explanation❤

[@srobles1660]

EXCELLENT WORK!!!

[@harp6198]

Thank you very much! This was excellent!

[@supermunheeb]

Gonna do this tomorrow this helps me a lot! Thanks.

[@reconeix]

Excellent, detailed, and approachable presentation. You're a natural educator!!!

[@christiankemakolam7028]

protocol simplified and made interesting. kudos

[@natalia8414]

Great video!😊

[@brilllance8903]

perfect video. clear narration. overall 10/10

[@nurmuhammad4540]

Excellent, thank you so much!!

[@sebastiantraxler5550]

Amazing video!

[@blacktear5388]

This was super helpful, thank you so much

[@haleemasadia5225]

Thanks a lot Mam for such well illustration! It helped me a lot as I'm a student of MS biochemistry

[@btssketch937]

Thanku so much for this amazing video I really appreciate your efforts ❤❤

[@UnKnownVlogger01]

Every student like me deserve an advisor like you Mam 😅 Very well explained

[@isabelasoares1168]

Thank you for this amazing video!!! This channel will help me a lot

[@md.1276]

Don't get me wrong but I was here for science but stayed till the end for her smile

[@michaelokeke7783]

Amazing! I love every bit of this video; so refreshing!

[@shubhamaryan2972]

great explanation...

[@WeDream18]

Amazing explanation...❤ It covers each and everything you need to know. loves the way she explain.

[@03500]

It was amazing, can’t thank you enough 🙏🏻🙏🏻

[@parisahouri]

The best! Thanks a lot 😊

[@salmanashraf8790]

Super method of explaining lady (teacher)

[@aamnasajid6570]

the best video I ever saw

[@MichaelSmith-ij2ut]

Hours of lecture and explanation distilled in to 15 minutes.

[@DiemNguyen-gg8gy]

Thank you very much for the excellent video! It is very helpful!❤

[@hana-al]

It was excellent !! thank you ❤️

[@rufinamenezes7712]

An amazing and fun way of explaining. Thank you very much. Please continue it so that many may benefit from your explanations

[@Brume20]

Amazing video.

[@alsaqar1]

Best video in this subject 🎉🎉

[@user-lp5qe1tv7d]

Loved it thanks😊

[@UdhayaBalaKanna111]

Just amazing!

[@maxmordente2218]

Thank you!

[@ayeshakhan279]

Thank You!

[@minahil21]

Very well explained mam

[@Omar-Khaairy]

Thanks so much

[@taylormarkel1094]

thank you so much!!!!!

[@arianevitoriasilvestrehann5140]

I loved it!

[@kadircanvural6814]

awesome! thank a lot

[@shreeramesh9902]

awesome

[@Ganesh_lamichhane]

How beautiful your video like your smile. I liked your video and your smile. Keep smiling and make videos.

[@alhaeri1]

legend

[@rogersundeen4022]

Everybody got this? It's going to be on the test.

[@omkarmore7613]

Very amazing with a very pretty mam

[@wednesdaysfrog8422]

Which kit have you used?

[@ghazalehgolmohammadnezhadk5307]

What happens if I forgot to wait for 1 minute before the last centrifuge?☹️

[@richardlange7559]

Some of that equipment is filthy!

[@lauraschonborn5633]

You are so sweet and cool 🎉

[@ThatStickyNote]

Please do not inoculate your bacteria by poking a pipet tip by hand. Hold the sterile pipet tip with sterile tweezers (hold the tweezers in a blue flame for a sec) and then poke the colony on the agar. Also work under a flame or in a lateral flow cabinet.

[@LabXchange]

Thanks for the feedback! In this video, Molly is working with media that includes selective antibiotic. If she were working without antibiotics, she would have used sterile tweezers. It's important to note that best practices can vary, so you should always check with your PI if you have questions.

[@badangel842]

I little bit confuse for Explanation O_o

[@juxyclips671]

Whats her @?