RIBO-seq analysis pipeline: Snakemake tutorial

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Marcos Morgan

Marcos Morgan

Күн бұрын

Пікірлер: 2
@SubramanianThothathri
@SubramanianThothathri 9 күн бұрын
Can you please make a video from the top to bottom analysis for Ribo seq? I see you mention that RNAseq is paired with Ribo seq. How does that work? Does the workflow during analysis integrate the same? Loving the content!
@MorganMarcos
@MorganMarcos 8 күн бұрын
Thanks for the comment. I am glad you are finding the content useful. The pipeline in this video prepares the RNA-seq and the ribosome footprinting count matrixes in parallel. However, our implementation provides the RNA-seq count matrix to Xtail instead of DEseq. Internally, Xtail will run the DEseq for the RNA-seq and ribosome footprint count matrixes and then compare them. This video explains the strategy used towards the end kzbin.info/www/bejne/pIvEi2muppxnf68.
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