Rose tissue culture propagation

We have started work on the Glowing Rose, which was our stretch goal. Here's Kyle showing you how we make a rose tissue culture.

[@inayahrizki7932]

Feel happy after see this video for my online practicum

[@theflaca]

In 1985 I was employed in a tissue culture business propagating all manner of profit making plants. We even successfully propagated roses which even flowered inside the flask. Incredibly the mini bushes looked like they belonged in Lilyputia.

[@danieljheelan5256]

Hi, Was you using your own home made medium?

[@theflaca]

@@danieljheelan5256 yes it was the property of the company. But rose lines were shelved for more profitable plants. Though we did clone gum trees, and other natives. Ron de Fossard pioneered gum tree media. Look him up. As I was so young, I took it all for granted. Now I wish to return to the industry. The big cost was always the power bill. But now with solar panels, it can be more profitable.

[@jyvaineorchids2255]

@@danieljheelan5256 hi Daniel, i know you from orchidées 91, i do in-vitro too, i think you'll reconise me lol ☺️. It's funny to find you on KZbin like that.

[@jyvaineorchids2255]

@@theflaca hi, wich company were you worcking at ? I could as questions about micropropagation.

[@mowaisshahid]

Sir, your effort is really appreciable, but it would be very nice of you if you add up more detail.. I work with roses daily, and in my lab i use these techniques: 1) taking ex-plant tissues 2) put them in a jar covered with cloth under running tap water; washing with 70% ethanol for a minute 3) getting the Laminar Flow Unit (LFU) ready for work 4) Inside LFU: surface sterlization of the tissues in 20% Sodium hypochlorite for 10 minutes; and then rinsing with ddH2O 5) Inside LFU: growing the tissues (for shooting) in media, 1L of which includes: Sucrose: 30g, MS: 4.4g, 6-BA: 1ml, GA: 1ml, NAA: 50 ul, Agar: 6.8g [pH 8.5] [Media sterlized at 115 degree C for 15 min and poured to jars inside LFU] 6) Incubate the jars at 25 degree C for 1-2 months 7) Inside LFU: growing the shoots (for rooting) in media, 1L of which includes: Sucrose: 30g, MS: 2.2g, NAA: 100ul, Agar: 7.5g [pH 8.5] [Media sterlized at 115 degree C for 15 min and poured to jars inside LFU] 8) Keep growing the plants until big enough for the jar * If you don't want to transfer the plants to soil, just cut the roots [inside the LFU] and regrow them in rooting media * If you want to propagate from invitro-to-invitro, then skip step1-4 * Sterilization of the LFU with UV (30 min) and then 95% ethanol is very important. Also sterilize all the material [blades, scissors, etc] inside LFU Good Luck

[@badarbanoori1280]

Info is incomplete

[@danieljheelan8966]

Hi, Thanks for sharing. If you have more info, I am interested in. Actually have a glovebox and I am working with orchids seeds but too many contaminations. Planning to build a Laminar Flow unit. Daniel

[@danieljheelan5256]

Thanks for sharing your experience. Daniel

[@danieljheelan5256]

Hi, Thanks for sharing your experience. Can you, pkease, give me some more details on the culture media. Thanks

[@geos7432]

Hello sir, thanks for the info. When you say 6-BA :1 ml GA:1 ml what is the concentration in one liter. Thank you in advance.

[@user-jb1vh9sh6g]

Thank you very much from Yemen concise but very useful information about plat tissue culture

[@retiredlogman]

It would be nice if you could add some more detail and also do a follow up visit as to what the next step is after these initial steps. Your approach would make propagation of difficult to root roses easier.

[@daaaanggggnhat99]

+largelettersize Just from experience miniature roses like that one he has, which is the variety Honora by the way, are relatively easy to root. You just need to stick a cutting in rooting hormone, soil, and put a mason jar over it. In fact they are simply massed produced and made for a week or so of entertainment before they die from root rot and insufficient growing conditions from overwatering by the buyer.

[@v0nnyboy]

Oh my god ... YESSSSSS!!

[@danieljheelan5256]

Hi, Thanks for sharing. Could you tell me what medium you are using and if possible to get the list of ingredients and quantity. Thanks Daniel

[@carmendompig6804]

Very interresting,how.can i learn this.

[@danieljheelan5256]

Hi, I need some more info on your sterilization process and the media you are using. Is it possible to get it. Thanks a lot.

[@carmendompig6804]

Do you have the Agar mix lecture tot preparing ,i like to do this at mine home ,because i have a lot roses in my garden

[@amtd3808]

Any update on results ?? Are they grow or contaminated ???

[@gauravbahadurkhadkachettri4234]

Sir how do u make cytokine hormone

[@jyvaineorchids2255]

Ok but what is the formula ?

[@bbsppkhanapur3119]

May I know the composition of your special medium

[@SourabhKumar-ml8zn]

What is this gell .

[@thunorrr]

What is the medium composed of?

[@darkquaesar2460]

Rooting hormone and agar gel with a mild compliment of plant fertilizer probably.

[@MuhammadFaisal-rk8cc]

Hello sir can we do tissue culture what is dragon fruit???

[@danieljheelan5256]

Hi, Thanks for sharing. It seems that you are using your home culture media. Can you give me the recipe for your medium. Thanks Daniel Jheelan from France

[@doingcrazystuff132]

In what environment should we keep this jar?

[@darkquaesar2460]

room temp out of direct light. Once roots develop you can just take it out and plant it like any other plant. It's simple propagation just on a very small scale.

[@SuperLazyCat]

What is the secret gel? Just some cloning gel?

[@darkquaesar2460]

Rooting hormone and agar gel with a mild compliment of plant fertilizer probably.

[@theflaca]

As it's a bit milky, it's agar. The gels are actually very clear, but can produce very callousy cultures and gigantism. One product used to be called Gelrite.

[@danieljheelan5256]

Avoid the plant to fall and go to the bottom.

[@anupmondal2951]

sir pls tel me ,full name of -ms ,Ga,6-BA,NAA

[@phd_unispeed3350]

You are not desinfected the Stem beforo the culture !!! Why you are not sharing your science for all scientist ?