How to Tissue Culture an Orchid from Start to Finish | TC Made Easy

[@walteregosson4833]

hi! did you make any update please?

[@spudgn]

Thank you. I will use this to propagate my vanilla orchids.

[@_c_y_p_3]

You absolutely have to do an episode with Dickson Despommier and Vincent Racaniello from TWIV, yes I know it is a Virology podcast but the platform those two built , with new shows like Matters Microbial they intersect with yours in major ways and such giant resources for the people who love staying up on discovery week by week, paper by paper. i know everyone has a precious list of advice but I sorta think you could expand your viewers and I know Vincent is all about it.

[@FreshfromtheFarmFungi]

Hey nice video! I have a similar lab setup for mushroom cultivation and wanted to get back into plant tissue culture and so glad I found your channel, I appreciate the detailed video! My advice from past experience is maybe the initial cuts were not as sheer/clean as possible with those scissors - try using a sterile scalpel blade in a petri dish while using the forceps - it could help with the contams and tissue die off by making a less damaging cut

[@plantsinjars]

I don’t use a scalpel :) if you watch some of my other videos you’ll see why lol

[@jorgegti80]

Thanks for sharing this information I was looking for it can you do one of the plantains or banana plants 🙏

[@plantsinjars]

I’m planning to TC some musa aeae’s and cavendish bananas later this summer!

[@jorgegti80]

@@plantsinjars thanks 🙏 will be waiting for it and hope you hand heals quickly.

[@hotrodAndy]

I have had luck using that media for carnivorous plants!

[@alessandroraggi785]

Which part do you use for breeding cattleya? you are very good, congratulations

[@charliemagoo7943]

Details on the scale removal???

[@ivanacoelho3261]

Hi there. Great vídeo! Will there be an update? Do you still use that medium for orchids? Thanks! And congrats for the excellent vídeos!

[@plantsinjars]

Thanks! I redid this experiment a year later and I have about three keiki's per node. I used the same media since I had it left over from this video

[@fishmut]

Hi there plants in jars , love your videos , I’m thinking of buying a tissue culture kit just to do this as a hobby, love to do mushroom culture in Petrie dishes to and develope the spores to make more , tried it once and failed lol wasn’t sterile enough , lessens to learn hey . I do think I can do this , very fascinating . Tissue culture plants will be loads of fun , I get a buzz out of things like this. 👍

[@walterramirez5576]

Not hating or anything but is there a reason why chose the orchid multiplication medium as opposed to the orchid stem propagation medium?

[@danieljheelan5256]

Hi Laur, Any update? Planning to try again. I saw your new video in the university and it is possible to clone from the flower buds. Did you tried? Will it be possible to get some more info on it. Merci.

[@janvi2283]

Do we have to use any chemical to break the bud dormancy in MS media?? My Dendrobium bud got stuck after some time and it's not developing

[@chrissmith7259]

Dendrobium is going to be my first

[@ashndz]

@plantsinjars Ive enjoyed the organized and detailed presentation of your work. Would you share the book information you mentioned in the video?

[@plantsinjars]

It's called "Plants From Test Tubes"

[@Mario-yt7ni]

3:17 what do you think about sterilizing the plants with e.g. 6% H2O2 instead of bleach? could we also put the plant material cleaned with H2O2 directly into our culture medium without cleaning with disinfected water first? I have seen in vitro plants that were in water with prmanent 2% H2O2 and they looked great.

[@Alexandra_Yasneva]

Расскажите подробней как вы стерилизуете экспланты ?

[@robertaburatto4140]

Hey, do you have a protocol for planting seeds? Do you use hormones? Thanks for the videos

[@robertaburatto4140]

I mean, orchid seeds

[@MatthewOConnor-fb4ts]

When you put the jars in the autoclave, are the lids just resting ontop of the jars as a cover or are they screwed on tight?

[@plantsinjars]

Just resting on top

[@AndrewChannelXD]

Could you pls make a tutorial about growing ghost orchid with tissue culture? I was wandering when I saw the video about the Venus flytrap propagation that these orchid is also in great demand an great risk of extinction, they don't need air movement so maybe these would be a good solution

[@AndrewChannelXD]

@plantsinjars

[@plantsinjars]

Idk if I will ever make a specific tutorial but I am currently growing ghost orchids :) Unfortunately the main supplier of them in Florida is an a$$

[@odysseyorchids9507]

Is there a link to your laminar flow hood ? I have an old homemade one that could Probly get updated.

[@plantsinjars]

It's the FLOW Series Laminar Flow Hood, 24" from Air Science. I really like it! ductless.com/catalog/product/view/id/113/s/flow-series-laminar-flow-hood-24-600mm-nominal-width-comprising-of-a-flow-24-head-a-and-a-flow-24-encl-115v-60hz-north-american-cord-set-unless-specified-includes-one-asts-030-hepa-filterflow-24-a/category/4/

[@h1234e1234]

Hi!, it was a very good explained video! i really loved it!. I want it to learn how to plant orchids but i cant find any books nor information, can you tell me what is the name of the book where you learn to cultivate orchids? Thanks and a hug from Brazil >D

[@plantsinjars]

There is a book called Plants from Test Tubes that is really helpful. It's not specific to orchids but it does have lots of good information about tissue culture!

[@raybrumett6599]

Can you tell me what book you have on tissue culture Ray

[@plantsinjars]

Plants from Test Tubes is my favorite reference

[@hypericacea]

Nice dice

[@sneakythumbs9900]

how do you keep track of the orientation of your explants through the process?

[@plantsinjars]

If it's a plant where I can't tell just by looking at the explant then I'll keep one of the internodes longer to remember

[@Not-Sorry2011]

I can’t even keep an orchid alive. I’m jealous.

[@Cacti_Of_Mexico]

How do I dispose of the culture media after I deflask? Can I pour it down the sink?

[@plantsinjars]

I scoop it into a trash bag and throw it away. it only contains trace amounts of PGR’s

[@Cacti_Of_Mexico]

@@plantsinjars what about the other medias? Like the rooting one and the orchid seed starter?

[@plantsinjars]

Yes

[@Cacti_Of_Mexico]

@@plantsinjars ? What does yes mean?

[@plantsinjars]

@@Cacti_Of_Mexico As in yes, I would throw those away as well :)

[@treeislife93]

Could it be possible any simple home gardener to create new plant via tissue culture technique without enough knowledge? Like stem growing, airlyering and other grafting techniques ? Is not complex more ??

[@helixlabs-s2q]

No not at all u can grow plants via tissue culture with easy ways

[@danieljheelan5256]

Hi, As you, I tried to clone/multiply my phals but never succeded. Wanted to know if you succeded to multiply from the phalaenopsis flowers stalks. Thanks for your update. Daniel

[@plantsinjars]

I’ll make an update video next month!

[@danieljheelan5256]

@@plantsinjars Thanks a lot for your reply. I tried several times but never succeded. Tried different methods and media. Contaminations or burning the explants when not contaminated. When non contaminated, no germination and no callus formation. Daniel Jheelan from France

[@danieljheelan5256]

@@plantsinjars I am in a TC group with Frank T and will follow you. Not sure but was it you who started all laying and doing all on the floor.😀 Daniel Jheelan France.

[@plantsinjars]

@@danieljheelan5256 I think you're thinking of Tatum Sweet!

[@danieljheelan5256]

@@plantsinjars Yes, correct.

[@modernalien6949]

I dont trust my PH meter either. Those things are diabolical.

[@capnsmashem1554]

You avoided BAP on the other video I watched of yours, but this has it. Is it just sorta mostly dangerous unless you're careful?

[@plantsinjars]

If you wear gloves and avoid ingesting it / getting it in your eyes, then it's really fine to use. I knew a lot of people would be trying TC for the first time from my other video, so in that case I always recommend starting with Kinetin. Easier to dispose of, etc.

[@capnsmashem1554]

@@plantsinjars But keep in mind it's a Class 1 Carcinogen that CAN pass through skin. The Kinetin advice is genuinely very good.

[@diegocebrian2125]

how the orchid did go?

[@plantsinjars]

They grew shoots but then got contaminated. I needed to seal the jars with Saran Wrap

[@nick1f]

@plantsinjars Even a video with the mistakes you made and lessons learnt in the process can be useful to us.

[@RomainQ]

I have the exact lack of trust in my pH meter hahah... I dread that step

[@muddypawswanderlust]

I used the following explant sterilization for J.regia (persian walnut tree) --> 30sec in ethanol 60% solution then washed 3 times in sterile tap water then 30min in a mixture of captan and benomyl fungicide (each 1gr/L) + 2mg/L streptomycin (antibiotic) and then washed 3 times and then 15min in a bleach solution and then again washed 3 times with the same water. -------------⚠️ but no matter how hard I try, my explants dies after a few days due to endogenous contamination. What can I do to prevent this? I even added 50mg/L streptomycin in my establishment phase medium but still no luck 😭 -ps: I use nodal segments of a walnut tree in the field as my initial explants

[@mhs1351i6]

درود مهیار جان.امیدوارم موفق باشید🌷🙏

[@muddypawswanderlust]

@@mhs1351i6 merci az shoma 🙏 shoma tissue culture e gerdu kar kardin? Va inke man ghablan shomaro didam? :)

[@mhs1351i6]

@@muddypawswanderlust اینقدر وسیله ها گرون هست و تجهیزات آزمایشگاهی گرون شده پشیمون شدیم کارکنیم.نه من به دلیل علاقه به کشت بافت نگاه میکنم فیلم ها رو دیدم هموطنم پیام گذاشته خوشحال شدم پیام دادم به شما🌷

[@muddypawswanderlust]

@@mhs1351i6 bale sarmaye ziadi mikhad vali ye seri taghizat ro khodetoon mitunin besazin. Man laminar flow hoodamo khodm sakhtm :)

[@muddypawswanderlust]

@@mhs1351i6 man kolan reshtam computere va aslan az giah sar dar nemiovirdm vali tu yeki do sale gozashte kheili alaghemand shodm koli paper khundm va karo shuru kardm :)

[@wendybedrosian5327]

Hi- is it possible for me to do meristem tissue culture at home? I’ve watched your tissue culture videos and I think I’m totally capable of tissue culture. I grow dahlias and I had to toss more than half my dahlias (I grow about 500, many rare) this year due to virus. One is my own breed that is named after a dear relative who passed away. I think it may be diseased and I am heartbroken. I think meristem culture is the only way to eliminate the disease.

[@plantsinjars]

With a microscope and lots of practice it is definitely possible

[@wendybedrosian5327]

@@plantsinjarsany videos you can point me to? From drawings, I can quite see where on a plant I would find the meristem.

[@wendybedrosian5327]

Also, what low cost microscope would you recommend?

[@EDDIEGARAGE]

none of your product are not available in Australia

[@TwoTreesStudio]

I HATE MY PH METER SO MUCH TOO. My calibration solutions never read accurately and it seriously pisses me off. If you ever find one that works right and doesn't keep people up at night please make a video about it.

[@plantsinjars]

The bluewave one is decent as long as you store it in kcl solution but it's so overpriced.

[@goober-ll1wx]

You're beyonnd cute...