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today our topic is sahli method so first we will discuss about what is sahli method principal and then we also study definition in easy way so you watch full video at till end. and learn everything in this biopsy video if you any doubt in this video so you ask me in comment box then I replied in shortly time. your doubt in any subject like hematology, microbiology then you tell me in comment box also.
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• hematology
sahli method theory :-.
Sahli´s Method
Sahli's hemoglobinometer is a manual device that contains a hemoglobin tube, pipette, and stirrer, as well as a comparator. Hydrochloric acid converts hemoglobin to acid hematin, which is then diluted until the color of the solution matches that of the comparator block.
Blood is mixed in a solution containing potassium cyanide and potassium ferricyanide. The potassium ferricyanide converts Hb to methemoglobin which is converted to cyanmethemoglobin (HiCN) by potassium cyanide. The absorbance of the solution is then measured in a spectrophotometer at a wavelength of 540nm
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