Salting In and Salting Out of proteins

[@ehatipo4598]

You're probably the best Biology instructor on KZbin. Thank you so much for explaining biological concepts.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@tribaltumblelove]

I have a biochemistry exam in 2 days lmao and this really helped me to understand things better. thanks mate.

[@zianaqash7714]

Suman your detailing to make things understand is flawless. It never matters how intricate the topic is. You are a real successful teacher.

[@KiEfErThEnOoB]

I'm taking a food science class right now, and this was great help. Thank you!

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[@shomusbiologyofficial]

Thabk you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@amitkarmakar1684]

Lunch korte korte bujhlam... Khub sohojai. Thank u. Your teaching skills are very impressive sir.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@ranjana9433]

Have been passing my exams only because of your videos! Better than how most of our professors teach. Thanks bro :)

[@matimbantimane3170]

I'm taking you with me to medical school next year, you're the best teacher.

[@damilolaolagunju213]

Thank you very much for this explanation. I'm watching it to prep for my exam tomorrow

[@shomusbiologyofficial]

All the best for future career. Stay tuned for more

[@jayashreejoshi7244]

I am a pharmacy student... It helped me with my subject... Thanks alot

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@rforrebel2687]

It was awesome...thankyou sir...

[@leenshammout2284]

Super helpful thank you so much 😍, Great teacher made it very easy and logical

[@shomusbiologyofficial]

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[@kaziazimuzzamanazim8941]

This is Super easy to understand. Thank you❤

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@thatdiyguyraymondmonk1225]

Thank you so much. I greatly enjoyed your presentation and understood so much more that all the papers I read on it.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@MissJamborina]

thanks!!! this helped us out a lot for our biochem exam!!!

[@ahgasezrz]

Thank you so much. I understand you more than my own lecturer 😅

[@shreeyashetty7514]

U r such a brilliant and u taught us very well it really helped me to get the concepts plzz make more such videos which is related to biochemistry thank you

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@asankaprageeth6245]

thank you very much. it will very important for m for my further studies

[@shomusbiologyofficial]

You're welcome

[@soumyasaxena5151]

please upload video on different methods of protein precipitation...and this was a really helpful video.

[@hinasiddiq3246]

really helped in understanding concept of salting in and salting out.....keep working

[@priyankakaushik5270]

Really ur explanation out of standing.. concept clear by watching ur vedio.. Thanks alot. Please upload vedio related to refolding and protein purification so that helpful for us.

[@harleenbali3620]

You make evry difficult topic so easy🌻thank you so much sir.

[@shomusbiologyofficial]

You're welcome

[@adarshkumarkormath5825]

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[@shomusbiologyofficial]

You're welcome

[@babrpr]

Great explanation sir❤

[@shomusbiologyofficial]

Thank you

[@shwetaathwani4756]

I'm thankful for uploading such helpful videos

[@MultiPratik09]

Keep spreading knowledge sir ..loads of love from mumbai ❤️❤️ thank u for such beautiful explainations

[@sheikhsahar3434]

it ws sooooooooo helpful...i never understood the difference...but after watching this...i am clear about it...thnku so much..😊

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Plzz give important topics about 12th clas biotechnology plz I really request

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You are superb....Thanku😊😊😊😊😊😊for this amaizing video...

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@shahistha7416]

I don't know how to thank you. your videos are amazing.

[@shomusbiologyofficial]

+Brave Girl Glad you liked my lectures

[@g4ganguly]

Really helpful for me...thank u sir...thanks a lot 😊

[@shomusbiologyofficial]

You're welcome

[@manishjackson6563]

its great help for students thank you

[@ChilangaBanda08]

Great explanation! Thank you, greetings from Houston, Texas...

[@shomusbiologyofficial]

+ChilangaBanda08 thank you. Glad you liked it

[@prabirbarman1702]

Thanks sir for this wonderful video.

[@LeXaa95]

Absolutely brilliant! Thank you,it helped me A LOT!

[@shomusbiologyofficial]

+Alexandra Gherman glad you liked it

[@Frenchfemme_Dharna]

Sir, u r best, yr lecture helps a lot..

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@khanyisilemtala1815]

Woooow! this is so informative.Thank you so much #Bless you

[@shomusbiologyofficial]

+khanyisile mtala thank you too. I am glad that you are getting benefit from the videos

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Thank u you so much. Helped alot

[@shomusbiologyofficial]

You're welcome

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You are truly amazing!

[@manicpixiedreamgirl7930]

Hey, Thanks for this video. It's a great explanation. I just wish the sound quality was better.

[@shomusbiologyofficial]

You're welcome

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Thank you so much.... It helped us a lot.... Looking forward for more such informative videos👍😁

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@Erram132]

Excellent explanation

[@shomusbiologyofficial]

Thank you

[@srrajeshmahapatra7632]

very nicely explained... thank u sir... for ur efforts..

[@maryamyousaf700]

It helps me a lot Thank u sir

[@shomusbiologyofficial]

You're welcome

[@kayecrypto4594]

thank you so much for this I finally understand the concept :)) 😊

[@shomusbiologyofficial]

You're welcome

[@niranjannayak6149]

Next level ❣️

[@shomusbiologyofficial]

Thank you

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thank you sir for your prestigious help...

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wow ur lectures are amazing just keep doing so sir the people will continous to subcribe ur chanal

[@umairbashir7049]

Brilliant, brilliant, brilliant.

[@shomusbiologyofficial]

Thank you

[@sharafwasima2839]

Thanks a lot... Now I understand it properly

[@swatimishra8119]

very nicely explained.

[@shomusbiologyofficial]

+Swati Mishra thank you.

[@apaarnasrivastava4126]

Awesome.....you are too good !

[@saitej9956]

Can you please make a video on ,in process quality control test for biological products?

[@xxvictor21xx1]

exellent explanation my friend !!

[@shomusbiologyofficial]

You're welcome

[@sandybell234]

Very helpful! Thank you!

[@shomusbiologyofficial]

+Sandybell Anorga thank you

[@ronaksinhparmar2288]

thanks 4 ur efforts dear.....nly things u should have mentioned that its because of few surface hydrophobic residues that have been exposed after water been removed from dat part n this hydrophobic residues tend 2 aggregate n result in precipitation...anyway thanks agn

[@Madhura-fn1um]

Very nice ❤🎉.....

[@shomusbiologyofficial]

You're welcome

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You are a legend!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@soumikasengupta9449]

Thankyou....It was really helpful

[@G.U.M.M.M.Y]

nice presentation

[@RiyaKumari-ft3ii]

Very helpful sir

[@shomusbiologyofficial]

Thank you

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You’re the best ❤️

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@laliabenmaiza9218]

THANK YOU VERRY MUCH

[@shomusbiologyofficial]

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[@InfiniteUniverse88]

Are the dead sea, the great salt lake in Utah, and the Don Juan pond in Antarctica considered high salt concentrations?

[@vedanshipal1308]

Thank you Sir!!

[@shomusbiologyofficial]

You're welcome

[@vedanshipal1308]

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[@maheenchoudry4773]

so informative

[@shomusbiologyofficial]

You're welcome

[@just_learn_it]

Thankuuuuu soooo muchhhh sir🙌

[@shomusbiologyofficial]

You're welcome

[@amrutavarshinijoshi7098]

Hey thank you this video helped me alot 🙂

[@EnnaRagashiyam]

Nice lecture....... Thank u so much.

[@shomusbiologyofficial]

+Gowsalya Shiyamala you're welcome

[@nba5890]

Suggest a hypothesis to explain how increasing the salt concentration of an extract of 10nm filaments could form a solenoid. Can i get the answer of this question

[@lakniehasara3208]

Sir, After adding same amount of saturated ammonium sulphate solution to the protein sample along the wall of the test tube, what is the reason for appearing a white coloured ring in the interface?

[@Reni8705]

YOU are the Best 🤓 Thank You

[@anupamanusanka7611]

Thank u very much really understood clearly

[@shomusbiologyofficial]

You're welcome

[@UshaUsha-ng5cx]

Really thank you so much sir

[@kaoutarkh8895]

thank you so so much it was very helpfull

[@shomusbiologyofficial]

+kaoutar kh thank you. Glad it helped

[@mubasharkhan9795]

thank you sir

[@lynamrabet2936]

Thank you !

[@akashgayakwad8983]

nice video. can you have the video on protien(water soluble) interaction with water.

[@shomusbiologyofficial]

Thank you. Sure I will

[@akashgayakwad8983]

@@shomusbiologyofficial if you have please share with me , I want learn this thing as you explained.

[@sidrashaikh4833]

thanku soo much sir

[@shomusbiologyofficial]

You're welcome

[@arpittyagi6404]

Thank u so soo much sir..

[@shomusbiologyofficial]

You're welcome

[@vivekchamoli2063]

Very helpful video nice.

[@vishalupadhyay416]

But sir denatured protein self insoluble or ptt due to hydrophobic side chains of amino acid which makes hydrophobic bonds in protein for folding and bending and this hydrophobic bond break by SDS then hydrophobic side chains expose in water which makes protein ppt due to hydrophobic interactions between protein and protein. so how does potassium acetate ppt denature protein????????? Please explain with basic details or make a video on this topic sir, please?

[@danieltapia1153]

So good!

[@nicholasjay3932]

This video was helpful in understanding the chemistry, is there anyone out there that could provide sources applying the same information that is applicable to how salting in and salting out works with brining meats?

[@veena6586]

Sir, is it similar like to make inside out n outside in vesicles???

[@tsegayteklay7898]

tnx it helps alot

[@shomusbiologyofficial]

You're welcome

[@demialien]

this was really well explained, thank you so much! I have a question, will this precipitate all proteins without differentiation? or will it depend on the concentration of salt we add?

[@humairahr3401]

Thank you Shomu :)

[@Sunny_alpha_male]

Perfect ❤

[@shomusbiologyofficial]

Thank you

[@rajeshr4115]

How to calculate Volumetric flow rate and linear flow rate calculation.. take one class sir please

[@phulmotichaudhari1900]

Thank you sir 👌. But i m little bit confused bcz higher the molecular weight of protein,lesser the amt of salt req for precipitation?Why sir?

[@CNkids73]

Excellent

[@shomusbiologyofficial]

You're welcome

[@anjalikushwah4448]

Easily understanding thanks sir

[@shomusbiologyofficial]

+anjali kushwah thank you. Glad you liked my lectures

[@brendaagyekum8681]

it was very helpful..thank you very much..but what happens to the salt added to the solution? how does it leave the solution?

[@vishalpanwar2108]

Brenda Agyekum #dialysis

[@manmeetsingh9936]

awesome bro

[@Aniltharalla]

How can we collect only one protein of intrest from salting out? As you mentioned in the video about purification of single protein by salting out but here after salting out all the protein complex mixtures will going to bind to each other. So here my question is how to extract the single protein from salting out method? Thank you

[@angelabineddy6055]

Shomu, in reference to long term side effects of anti-depressants, it is said that chemicals enter into the nerve cells and bind to proteins in the nucleus to create long term/permanent effects on the nerve cell function. I have been researching for years because I developed such effects, along with thousands of others. Can salting-out potentially remove these bindings and correct the damaged dna of the cell?

[@shomusbiologyofficial]

+Angela Bineddy I don't think it can be used to correct damaged DNa. It can purify proteins.

[@angelabineddy6055]

If it purifies a protein, removing whatever attached to it, then wouldn't that be changing the function of the cell back to normal? Before a chemical attached to it, which in turn changed its function.? I appreciate your knowledge, I'm so desperate for a breakthrough..there's not a day that goes by I'm not effected by it.

[@mubasharkhan9795]

+Angela Bineddy if we will able to use such salt which can dissociate the interaction of chemicals with protien of nerve cells' nucleus ivitro.. then it might work

[@planckhouse4418]

can u put classification of protein on derived protein

[@farahbenaissa5726]

Thaank youu

[@shomusbiologyofficial]

You're welcome

[@sciencenerd752]

thank you sir. i have done my bsc microbiology. do you suggest msc in microbiology or biotechnology?

[@shomusbiologyofficial]

Microbiology

[@sciencenerd752]

@@shomusbiologyofficial thank you so much ☺️

[@hempriyatiwari5535]

Is desalting and salting out are same?