Sanger Sequencing Data Analysis

[@WernTheDRUID]

These explanations are amazing

[@ericwuluglayjr6060]

Very helpful Teacher, thanks.

[@navyaudaya9973]

Lovely explanation. Thank you very much

[@ProfBeckmann]

Glad it was helpful!

[@zandilelanga4003]

Thank you so much for this. I find this first part of sequence analysis fairly easy. But I'm not sure what needs to happen after I've blasted my forward and reverse sequences. Can you please help with sequence alignment as well. I'm currently working with ITS2 and COI sequences.

[@ProfBeckmann]

what are you trying to figure out? if you are running coi i imagine you are just identifying species? so whatever is the top blast hit / is probably the species.

[@LauraIsabelRodasZuluaga]

Is there a way to view the Forward and Reverse sequences at the same time in Chromas? Do you know of any software where this can be done?

[@maryamrezaei-gazik2751]

It was helpful. Thanks.

[@NurArshad]

hello, can you suggest any software like this compatible for mac M1

[@lafina_aily]

Sir sorry, I want to ask, if the sequence data is in PDF format, how can it be converted into a ChromasPro file?

[@paveethrakunasegaran2025]

Thank you for the explanation. I have to sequence the Nucleocapsid gene of SARS-CoV-2 using the sanger sequencing method too. But, I am not sure how to interpret it as I sent sequencing for both forward and reverse reads. Now I have 2 data, a set of forward reads sequences and another reverse read sequences. Can you please help with which read should I compare with my reference sequence (Wuhan strain)? Forward or reverse? I try to align the forward reads with the reference sequence, however, the ending part of the sequence were not complete. So, how I can use the reverse reads for this and compare it with the reference sequence? Thank you.

[@ProfBeckmann]

you have to use something like NCBI BLAST to blast your data against the sequence and find where they match.

[@bidwansekhar]

❤

[@suryapanikar5824]

sir i need ur help

[@ProfBeckmann]

?