DNA Extraction Explained

[@ScienceMom]

Join our Kickstarter and get a fantastic 2020 Science Calendar! www.kickstarter.com/projects/sciencemom/science-mom-explains-the-atmosphere?ref=22gpz7

[@asmiterea377]

if you add the rubbing alcohol fast as in dumping won't it break the DNA due to the force ? so how it helps get a thicker mass of DNA ? Also in our class, our teacher asked us to pour it gently..we used kiwi . Why gently pouring alchol helps?

[@alexturner9841]

Thank you so much for doing the lab and providing the explanations! Since we are learning virtually, we cannot perform the lab together, so I have required my students to watch your lab for extracting DNA and also the follow-up discussion. Not quite as good as doing it in class and hands on, but pretty close! As an additional note I prepared a worksheet based on the lab which required answering the questions why each step was done: for example why add dishwashing detergent? - to dissolve the phospholipid cellular membrane.

[@christopheraranci842]

Awesome video series! I usually do this lab with my students, but unfortunately that won't be happening this year. It's hard to find a video that also explains the REASONING behind each step in this lab. Excellent job!

[@ScienceMom]

Thank you!

[@MardinMuhammed]

This is so underratedddddd, great explanation!!

[@fresto2208]

Great video. Straightforward, enthusiastic. Thanks.

[@reeha3465]

This video was very helpful , giving answers to all my queries

[@ScienceMom]

A quick announcement about comments: When I first started visiting schools I would get a lot of comments saying things like: "Remember me? I'm John Doe from Mitchell Elementary! My teacher is Ms. Smith!" For a while I replied to these comments and then deleted them to protect the student's privacy--but then I came up with a better idea: Now when I do school visits I have the class name some part of the demonstration - then I let them know that if they want to leave a comment, they can reference the name that they chose and is unique to their class. Naming a ball or balloon is a fun exercise and also a good opportunity to talk about internet safety. So if you see things like #Bob or #teamShocky or "Michael the balloon!" and are wondering what in the world is going on.... that's what's going on. Also, since many of my viewers and fans are kids, any comments with vulgar language or curse words will be deleted.

[@nateleslie2725]

let's hope and pray I pass my bio test tomorrow

[@ScienceMom]

Good luck! 🤞

[@rasleenkaur1821]

you are great science mom

[@ScienceMom]

Thank you!

[@fjejjdbdndjdndndytbndndndn2232]

TBH you where at my school and the fifth graders came and whatched the ballon and all of that it was fun so I subscribed

[@ScienceMom]

Thank you! Glad to hear it.

[@Archaeopteryx128]

One of the things I find surprising is that DNA was discovered back in the 1860s.

[@brucettaylor]

Great video

[@jardinrecto]

wow! very nice explanation!

[@MohammadSalah1]

Awesome video, thanks 😊

[@asmiterea377]

if you add the rubbing alcohol fast as in dumping won't it break the DNA due to the force ? so how it helps get a thicker mass of DNA ? Also in our class, our teacher asked us to pour it gently..we used kiwi . Why gently pouring alchol helps?

[@nnennagrace8362]

What are the equipment and material for DNA extraction and sequence especially when you want to carry out DNA extraction and sequence for microbal communities associated with fermented cassava

[@angelaroberts1929]

Have you experimented with adding meat tenderizer to add proteases for better extraction from other fruits?

[@jilltaylor3568]

I enjoyed the explanation. Hard to believe a strawberry has more DNA chromosomes than a human.

[@ScienceMom]

A lot of plants do! Polyploidy (more than 2 copies of a chromosome) is fairly common in plants. Wheat has 6 copies of each of its chromosomes.

[@flayzy3913]

is the process of isolating DNA differnt from different types of cells?

[@divadimond7230]

I am your biggest fan

[@omsingharjit]

How to cut that dna for Diy dna fingerprinting ?

[@omsingharjit]

What if i put strawberry dna to other fruit's cell ?

[@johnkenn5886]

What kind of polymers could we extract from banana cells??

[@curiouscookie7359]

What is the next step after the DNA is extracted? How do you identify the base pair sequence?

[@ScienceMom]

It takes some specialized lab equipment to do sequencing, but you'd start by using with what are called "primers," small specific sequences of DNA that have already been sequenced and then you would see which primers match to your DNA sample.

[@SKChacha1]

How can the DNA extracted from the strawberry be tested in the lab

[@gordonfiala2336]

the Laughy Taffy. where were the ingredients gathered from for your experiment? did you discover the sources of the ingredients (NOT the supplier*)?

[@ScienceMom]

Isopentyl alcohol and acetic acid are both fairly easy to obtain from a variety of chemical suppliers. To get pure forms of them, you'd want to source them from a chemical supplier. I don't know what our source was when I did this lab in organic chemistry in college, but it's a classic lab and very fun to do. If you look up "banana oil synthesis" you'll come up with several links to more complete instructions.

[@gordonfiala2336]

Science Mom Hmmm I am just curious what the chemical is a derivative of. Ty

[@gordonfiala2336]

I mean moisturizing powdered banana isnt very impressive XD but specifically, maybe the smells are like the sources Moisturized and sweetened with alcohol: is what I am thinking.

[@ScienceMom]

Yes. Banana oil is just one very small part of what makes a banana taste and smell like a banana.

[@gordonfiala2336]

Science mom It is also a very small part of what makes up a banana once you've extracted it. 0_0 XD Such as the scentless dried powder that becomes scented when moisturized. Particularly with aromatic or "breezy" fluid.

[@heidibochenek588]

Could you run the sample through gel electrophoresis?

[@Angy-hn7jm]

Can human dna be extracted the same way like fruit is?

[@ScienceMom]

Not very well. Slightly different techniques are used when analyzing animal DNA samples. But the basic principles are the same.

[@omsingharjit]

What means of active 12:30

[@joeklaps4708]

I think it means that it's moving around actively, so if u put a different dna in there it will change the taste. She said that if u put it in the section that's not active it won't change the taste, since it's not getting mixed in

[@estimatingonediscoveringthree]

“What exactly does this specific piece of dna do”, the idea that this can be determined is incorrect, dna is synergistic and not binary

[@gabi7769-c2h]

dont bases + asids make salt water?

[@omsingharjit]

10:47 plz do that exp again but front of camera

[@aadelami1980]

Hey Science Mom, Does the whole body have 46 chromosomes or is it each cell? If it's the latter, that would imply we humans have millions of chromosomes. Please clarify.

[@ScienceMom]

Each cell has 46, but they are the same 46. Every cell (except for red blood cells and the gametes (egg or sperm)) has a nucleus which contains the same 46 chromosomes (2 copies each of 23 chromosomes). Red blood cells don't have a nucleus at all, and the sperm and egg each have 1 copy of the 23 chromosomes. So in a human body there are indeed millions of copies of those 46 chromosomes.

[@aadelami1980]

@@ScienceMom Thanks for the clarification.

[@mystery_assassin8909]

Remember when Jeff and the earth flew XD that was fun! Tho we have tons of balloons stuck in our cafeteria would you care to use your leaf blower to take it off??? XD (its Bianca :) from the assembly today)

[@ScienceMom]

That was so fun! And I did notice those blue balloons up on the ceiling of the multipurpose room. Too high for the leaf blower to reach, I'm afraid, but give them a few weeks and they'll lose their helium and float down.

[@MariamLionking]

Is there an equation for this experiment? I've been looking all over the internet and I haven't found an equation for how the DNA precipitates when ethanol and salt are added, can anyone please help me?

[@ScienceMom]

There isn't an equation because it's more a result of changing attractions than changing bonds. The DNA doesn't bond to ethanol or salt. The ethanol and salt change the way the DNA interacts with water (weak polar bonds) and encourages the strands to become attracted to each other and clump together. This document might be useful in explaining it in more detail: physiology.med.cornell.edu/faculty/mason/lab/zumbo/files/ETHANOL_PRECIPITATION.pdf

[@MariamLionking]

Science Mom thank u so much..I really appreciate your help

[@jennifergreen6296]

#hawkie Thanks

[@ScienceMom]

Thanks for stopping by the channel! I had a great time at your school.

[@jmf2274]

you need salt