SDS PAGE | Part 1 - Gel Preparation | Hindi

This video is about SDS PAGE Gel Preparation process. SDS-PAGE is an analytical technique to separate proteins based on their molecular weight.
SDS PAGE | Part 2 - Principle: • SDS PAGE | Part 2 - P...
When proteins are separated by electrophoresis through a gel matrix, smaller proteins migrate faster due to less resistance from the gel matrix. Other influences on the rate of migration through the gel matrix include the structure and charge of the proteins.
In SDS-PAGE, the use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel largely eliminates the influence of the structure and charge, and proteins are separated solely based on polypeptide chain length.
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[@vinitchauhan1217]

Mam you said Mercaptoethanol use to break non covalent bonds to maintain primary structure but actually Mercaptoethanol is use to break disulfide bond which is an covalent bond . can you just explain if i am wrong?

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Mam kya Resolving Gel , Stacking Gel Me Only Tris Buffer Dalte he yaa Tris - Hcl ??? Khi khi pe Tris- hcl bhi he 😢

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[@botanypedia4461]

mam you have expained very well but i have a question that you didn`t told about n,n metylenebisacrylamide use in gel making , if it is not used or it have optional usage in the gel making process . please reply .

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[@krishnaofficial316]

Ma'am apne to yeha pe do bar B marcaptoethanol ka Function bol chuka hay one is breaking of non covalant bond of protein & breaking of disulfide bonds , But actually the function is the second one. Please Clear my concept regurding this . 🙏

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[@suganyasivakumar6972]

Hi .. can u please tell us why the ph is maintained differently in stacking and resolving gel

[@krishnaofficial316]

I think, due to the zwitter ion effect of Running buffer i.e. Glycine the pH is maintained.

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Why we use less concentration of acrylamide in stacking gel in comparison to resolving gel ??

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Resolving gel k ingredients ks cheeze me add krty hain?..ks lab equipment me?

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Mam concentration vs pore size both part may different ho gya. if i am not wrong.

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Mam why do we use only glycine can we use any other amino acid in running buffer

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[@koyelmahapatra2959]

Maam 1 question hay aap 1st part me kah raha ha ki resolving gel me polyacrylamide conc kom hota hay esi liye pore size jada hota hay but 2nd part me kah raha ki conc kom to pore size kom , maam please clear this