Separation of plant pigments from spinach leaves by column chromatography

Рет қаралды 15,200

Күн бұрын

Пікірлер: 23

@nikulpatel8216 Жыл бұрын

"Thank you so much sir for creating this video! Your clear explanations and step-by-step demonstrations have truly helped me understand the concept better. I've been struggling with this topic for a while, but now I feel more confident thanks to your video. Keep up the excellent work and please continue making more educational content like this. You're making a positive impact in the lives of many learners like myself. Grateful for your effort!"

@dr.amolbkhade3003 Жыл бұрын

Great to hear these videos are making impacts on learners like u. Surely will continue to bring more such videos in the coming days. Thanks for complimenting and watching these videos. Regards

@chetanpulate4548 6 ай бұрын

Very excellent idea and demonstration sir. Keep it up

@dr.amolbkhade3003 6 ай бұрын

@@chetanpulate4548 Thank u so much for your comments and watching the video.

@bsc-Chemistry_Classes 6 ай бұрын

What was the eluting solvent ?

@dr.amolbkhade3003 6 ай бұрын

Dear viewer plz watch the complete video we have mentioned the name of eluting solvent along with mode of elution. Regards

@bsc-Chemistry_Classes 5 ай бұрын

Can we use other solvent in place of n hexane

@dr.amolbkhade3003 5 ай бұрын

@@bsc-Chemistry_Classes Yes u can use any other solvent having polarity index similar to n hexane. For example u can try in petroleum ether or similar solvents

@bsc-Chemistry_Classes 5 ай бұрын

@@dr.amolbkhade3003ethanol ? No right ?

@dr.amolbkhade3003 5 ай бұрын

@@bsc-Chemistry_Classes ethanol can't be used. If u want to dry other solvents including ethanol, u can first develop a solvent system using TLC and they try.

@amandubey908 Жыл бұрын

Why is it important to remove the moisture from sample

@dr.amolbkhade3003 Жыл бұрын

It's very important to remove the moisture (which is adsorbed as impurity on the sample) so that it will not affect the separation/elution. Additionally when ur adorning sample on silica presence of moisture will not give u dry free flowing powder for sample loading in the column. Thanks for watching.

@42ayoo 10 ай бұрын

Thank you for your feedback. I apologize for the oversight in not mentioning the measurement of silica and other chemicals used in the experiment. You are simply adding to them why?please use the standard the columnn chromatography techniques

@dr.amolbkhade3003 10 ай бұрын

Didn't get what you wish to ask or convey. Plz state clearly to respond. Thanks

@dr.amolbkhade3003 5 ай бұрын

Measurement of silica is usually taken in 200 parts compared with that of sample, but by practice u can get the expertise and need not to measure exact quantity. Thanks for watching the video. Regards

@ch.harshart1457 2 ай бұрын

सोल्वेंट मै स्प्रीट् भी इस्टरमाल हो जा??

@dr.amolbkhade3003 2 ай бұрын

@@ch.harshart1457 जी हा, कर सकते हैं

@ch.harshart1457 2 ай бұрын

@dr.amolbkhade3003 ये तो हो गयी सूखे की बात। जूस में कैसे करेंगे? जैसे शराब् निकालते हैं? तुलसा की पत्तियों का जूस निकाल लिया, अब उसको पाउडर मे बदलना तो कैसे? प्रेसेर कुक्कर से उसकी भाप को ठंडा कर ले? फिर उसको सॉलिड मे कैसे बदलेंगे? उबाल कर?

@dr.amolbkhade3003 2 ай бұрын

@ch.harshart1457 व्हॅक्यूम rotavaopour use करके concentrate कर सकते हैं. Liquid sample also can be loaded, not mandatory to convert to solid only.

@ch.harshart1457 2 ай бұрын

@@dr.amolbkhade3003 rotavaopour par wo 40 degree par garm karte hain to uski asli cheej to bhaap main chali jaati hogi .

@ch.harshart1457 2 ай бұрын

कोरोना मे मैने केले, सेव, काजू बादाम डालकर सराब बनाई, उसको यूटूबर् वाइन बोलते हैं। असल में वो सिरका होता है। मैने पड़ोसी दो तीन ग्लास भर कर पिलाई। उसका लीवर बैठ गया 😀 , अगले दिन डॉक्टर के पास जा रहा था। किचन की स्लेब पर जहाँ वो रात को गिरती थी, वहाँ से pthhar आज भी जले हुवे तो कितनी तेज होगी, बाद में मेरी समझ में आया ये वाइन नही सिरका है, और एक दो dhakkan बहूत होता है। फिर मैने उसकी प्रेसेर कुकर इस्तेमाल करके अल्कोहल निकाला। तो जिस तरह मैने अल्कोहल निकाला था अगर मे तुलसा के पत्तो से निकालू तो क्या उसकी प्रोपर्टी बदल जायेगी या बेहतरीन हो जायेगी??