Serial Dilution and Plate Counts

[@IdontCareDontReply]

You count the colonies that form in the plate, that is why it is called plate count. With the colonies found on the plate you calculate the CFU (colony forming unit) and have an estimate of viable cells available per mL from your original sample.

[@LSI_MGA]

can you add a video that explains what do you do afterwards? how to analyze the plates?

[@leonardomelo4673]

They really like the color green.

[@Allysiaj2085]

Ghtvgbbt

[@Paganforge]

Right down to the roof of the R&D complex in Hercules, CA. www.google.ca/maps/@38.0255571,-122.2766188,3a,75y,323.16h,87.15t/data=!3m7!1e1!3m5!1sZ7M37LTwRXIynelM8B9yag!2e0!6s%2F%2Fgeo1.ggpht.com%2Fcbk%3Fpanoid%3DZ7M37LTwRXIynelM8B9yag%26output%3Dthumbnail%26cb_client%3Dmaps_sv.tactile.gps%26thumb%3D2%26w%3D203%26h%3D100%26yaw%3D70.26178%26pitch%3D0%26thumbfov%3D100!7i16384!8i8192?hl=en

[@memorya1972]

now i know what will i do to my bacteria. thanks for the info :) very well explained.

[@WorthlessWinner]

You count the number of colonies that grow there; that gives an estimation of the number of bacteria in the volume of liquid you inoculated the plate with. To get the number of bacteria in 1 ml, you need to account for the volume you put on the plate and the dilution. If you put 0.1 ml on the plate, you need to times that by 10 to get it in ml. If you were counting 10^-5 dilution, you need to times it by 10^5 to get it in 10^1. Cancel those out and you have Colony forming units / ml.

[@sclair10]

thank you, I need a refresher and your videos have helped enormously.

[@biannekaulf9221]

Sameeeee.

[@ahmadtalsaadi]

Dear Bio Rad Thank you for this videos, is it possible to upload let say part 2 video to this one showing who to count the plates after incubation? However some people describe below who to count the plate, I do appreciated, but it will be more complete to add plate colonies counts video to this one! many thanx to all

[@miraedo92]

thanks for making & uploading such a good video.It's really help in understanding of rDNA Technology subject. I'm having an exam soon.

[@ItsSunnySara]

how long should u wait for cooling down?

[@IsabelRodriguez-nv2ue]

Thank you so much for this video. It is very clear and easy to follow and understand.

[@oparaeberechi980]

Thank you for uploading the video is quite educative.

[@bukolajoshua8054]

thanks for the explanation

[@sleeplessbird]

Nice explanation, thanks for the video.

[@you-dont-know-me]

Where can i buy these wicked neon green gloves??

[@a.s9509]

Do you discard 100 microliters from the last (7th) microtube?

[@joelarnaud6023]

This way of dilutions are not supposed to be 10 minus 1, 10 minus 2, 10 minus 3 etc?

[@ssaha7776]

They are -1 -2- 3

[@vetaya4783]

Very informative , Thank you

[@lorenaviernes6269]

hello! this video is very usefull..:) and it helps me so much for my upcoming research. can i ask.. what can i substitute to lb agar?

[@sharonlizreji3090]

Phosphate buffer

[@aanagul8787]

thank you

[@user-wk2gp3tu8h]

Thank you sir

[@TayyabAli-xm2oi]

Well explained!

[@codyellsworth7927]

Totally rad-ical

[@NileshRoamingNITIAN]

When you love greenery more than anyone !!

[@thesensiblecoderguy]

very well explained

[@thangatamil3265]

kumarsarvam singh

[@omarito1991]

more of this videos on youtube and less justin bieber videos please :) thanks

[@innestri807]

Nice video 👍🏻

[@Hira_Hyuna]

Subtitle please.

[@VLogKeluargaArsyla]

Alhamdulillah hadir

[@mohitmahajan4021]

there is no plaque counting in this video

[@mohitmahajan4021]

ok

[@حكمتكاظم-د3ج]

This isn't plaque assay for phage quantification

[@JYOtiRaNJanMANgaRaj]

❤❤❤❤❤❤

[@TrouzzzerSnake]

why call it a plate count when you don't count the plates?

[@emmylouhaught8572]

It didn't say how the colonies are counted

[@rummanshafi6175]

Then u didn't tell how to count the colonies

[@BioRadEducation]

Hi, Sana! To count colonies, we recommend placing the plate lid-side down (so the agar is up and you're looking at agar underside) on a background that gives you good contrast between the agar and the colonies - for white colonies, a black background works well. Using a felt-tipped pen, dot each colony as you count; by dotting you'll know if you already counted a colony. If the plate has a lot of colonies, use the marker to draw lines on the backside of the plate to mark four equally-sized quadrants, count the colonies in one of the quadrants, and multiply by four for an estimated number of colonies on the plate.

[@ahmadtalsaadi]

Sorry: how NOT who