Alkaline Lysis Miniprep

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This video demonstrates the alkaline lysis method of purifying plasmid DNA from a bacterial culture. The small scale purification is also known as a mini prep. This method utilizes a microcentrifuge as part of the purification.
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[@abdulrahmanibrahimdansadau6774]

This is 11 years this vedio Was made, I find it very useful today. Thank you bio rad

[@ashutoshkosariya191]

Could you plz provide protocol for this experiment

[@tiggertiggatiger]

wow! this is super detailed! thankyou!

[@bryantchan4251]

very useful, thank you!

[@scottfabricant7140]

The washing solution used came from the bottle saying 5x, and also says "alcohol added". Was the washing solution diluted down to 1x before use? Why the (unnamed) alcohol, is this (presumably 100% ethanol) used to make it 1x?

[@scottfabricant7140]

Also, try saying "elution solution" as fast as you can ten times. See how far you get.

[@thestormcraft4012]

most of the time the ethanol content used for these is 70%