Simply Cloning - Supplement 3 - pDRAW32

[@twirlaway568]

Andriy, I love you!!! This video is an absolute gem! I’m a final year BSc student and I’m using this ancient programme for my lab research project. I had no idea how to create primers but you helped me soooo much so ily and you are the real MVP xoxoxo

[@ashwinnair4816]

Thank you Andriy

[@iarue]

I was just thinking shouldn't the BAR gene insert start at 1346bp because the xho1 restriction sequence stops at 1345bp.

[@iarue]

Instead of adding random sequences when designing the primer. Why didnt u just copy the xho1 site and about 6 sequences before the xho1 site.