Solid Phase Extraction (SPE) Tutorial

[@saileshkatara686]

Seriously this is the best video on yt as of now for spe

[@ssss-cz1nf]

I've read it one million times in my theory book of analysis and with a 8 min video I understood it better than ever

[@skiphoffenflaven8004]

You are part of a growing trend it seems.

[@richardwells5390]

Really helped me understand this concept better for my lab class. My instructor sent me a video which was very difficult to understand, but this was much more comprehensible. Thanks

[@douglashurd4356]

Thank you, very clear demonstration. Also, it was nice to have Ashmeeta there to represent the human species! 😀

[@woromurtiutami1527]

Thank you, iam an undegraduated student, and i use this methode to separated my peptide sample

[@Phenomenex]

We are glad if it was helpful! Please let us know if there are any other demonstrations you would like to see in the lab.

[@sunnyrainyday6820]

Im a Forensic chemistry student and if never done a Solid Phase Extraction. tho it does look very similar to a Resin Exchange Chromatography experiment that i've done but fortunatly for the sake of time the experimenter could control flow

[@riazhosein2394]

Really awesome job guys! Thank you!

[@cuterobot7836]

Thank you this help out to undestand better SPE . Really nice moderators

[@shabinafatma562]

I want this manual Solid phase extraction kit I.e. Spe cartridge and push rod. Can you provide me the complete set up at least cost

[@RugahGreey]

Hi is it possible to use this method for the determination of concentration of caffeine

[@richardjohnson7746]

Great video! I think the best so far I have watched. But I would like to ask a question: Is it advisable to use MeOH throughout if acetonitrile is not available?

[@AlexanderBuitragoSantanilla]

Awesome video!!! thx a lot!

[@137bob3d]

the column of C18 is only a cm or 2 in length. far shorter than the normal phase columns i've seen on vid's here on u-t. could you comment on this feature of C18 columns , please

[@aqdusshaikh1306]

Hey, can we perform this for extraction of Isoquercitrin from petals of Dianthus.pavonius?

[@gianlucafarina2825]

First of all, thank you very much for the tutorial. It is really useful. I wanted to ask if the conditioning of the direct phase columns is the same. Thanks so much again!

[@nkhumelenimulalo6741]

A really good and detailed video. I am just wondering if i need a large amounts of samples how do i go about. Like if my sample introduction system requires atleast 10m of a sample.

[@ucjakozicka978]

love the explanation thank you!

[@137bob3d]

the chatter ends at 3:38 ... then the experiment begins

[@skiphoffenflaven8004]

Awesome.

[@joshr.5199]

im sure its totally fine, and im just weird, but the micropipette sitting horizontally with the tip on drove me a little crazy

[@Phenomenex]

Hey Josh, we are so sorry about that! Which part of the video are you referring to? Because I am sure that would make us a little crazy too.

[@ShOOtinGliTteR]

@@Phenomenex 3:41

[@ShOOtinGliTteR]

4:03 to almost 5:00

[@ShOOtinGliTteR]

and again at 5 02

[@francoisburgay9002]

Before eluting the samples, do we have to dry the cartridge?

[@Phenomenex]

While you don't have to, it is an option to dry the cartridge before eluting the samples. By taking this step it helps further disrupt the stationary phase/analyte interaction to promote elution.

[@Wei-yv1pp]

Thanks!

[@AE-ben]

Nice!!!

[@WhitneyCollins-q5g]

Precious Alley

[@danielataylor6292]

What solvents are the dyes originally in?

[@Phenomenex]

Hi! Great question, for this demonstration we used an aqueous based dye solution.

[@danielataylor6292]

@@Phenomenex thanks

[@AndyU96]

@@Phenomenex Is that why you passed water through the column before you injected the samples?

[@AbrahamBarry-o9u]

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[@DennyRosasco-i5h]

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[@SharonJones-l1u]

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[@ScottClark-o8f]

Oberbrunner Mountain

[@BritneyKuniyoshi-o1b]

Hauck Isle

[@ajay_musical_artist]

Ashmeeta Chera an Indian for styling purpose she's just pronouncing it as Ashee Shera😂 for sounding like "Britishers" very bad and shameful it is! That you are shy to take your own name respect 🇮🇳 your culture and language (mother tongue) @the woman in the video

[@AndyU96]

THIS is solid phase extraction? It's just a typical column chromatography for god's sake. Why is it not just called column chromatography?

[@jimseldiesel1362]

why is your micropipette HORIZONTAL IN 4:18 noooOOOOO

[@slytherinda4669]

it only matters if there is fluid in the tip

[@skiphoffenflaven8004]

Instant critic.

[@ZephaniahNoah-c9i]

4212 Julien Gateway