(46) High Performance Liquid Chromatography | HPLC | Instrumentation of HPLC | IMA Lecture Videos

Download the "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Tests and other GPAT Materials - play.google.co...
The instrumentation of High-Performance Liquid Chromatography (HPLC) involves several key components that work together to separate, detect, and quantify compounds within a sample. Here are the primary components of an HPLC system:
1. Pump:
The pump is responsible for delivering the mobile phase (solvent) at a consistent flow rate and pressure through the chromatographic column. It ensures a steady flow of the solvent through the system.
2. Injector:
The injector introduces the sample into the HPLC system. It accurately measures and injects a precise volume of the sample into the flow of the mobile phase heading toward the column for separation.
3. Column:
The chromatographic column is the heart of the HPLC system. It contains the stationary phase (packing material) that interacts with the sample components as they pass through. The separation occurs based on the differential interactions of sample compounds with the stationary phase.
4. Detector:
The detector measures the separated compounds as they elute (come out) from the column. Different types of detectors are used in HPLC, such as UV-Vis (Ultraviolet-Visible), DAD (Diode Array Detector), fluorescence detector, refractive index detector, mass spectrometer (MS), etc. Detectors provide signals that are used to generate chromatograms.
5. Column Oven/Heater:
The column oven maintains a stable temperature for the chromatographic column. Temperature control is crucial, especially for achieving reproducible separations and optimizing the interaction between the sample and the stationary phase.
6. Data Acquisition System:
The data acquisition system collects and processes the signals from the detector, converting them into chromatograms. These chromatograms represent the intensity of compounds eluting from the column at specific retention times.
7. Mobile Phase Reservoirs and Solvent Delivery System:
The HPLC system contains reservoirs for storing different solvents that make up the mobile phase. The solvent delivery system, often a gradient controller, helps in mixing and delivering solvents at precise compositions and ratios to optimize separation.
8. Control and Data Analysis Software:
Modern HPLC systems are typically controlled by software that manages instrument parameters, controls the flow rate, monitors detector signals, and analyzes collected data to generate chromatograms and quantitate the separated compounds.
9. Pressure Regulator and Safety Features:
HPLC systems operate at high pressures to ensure efficient separations. Pressure regulators and safety features are integrated into the system to maintain safe operating conditions and prevent damage to the instrument.
10. Accessories:
Additional accessories such as degassers (to remove dissolved gases from the mobile phase), autosamplers (for automated sample handling), and different column types and dimensions can be used to enhance system performance and versatility.
Get in touch with the solution by just clicking the following links-
Facebook Group- / solutionpharamcy
Mobile App - play.google.co...
New Channel (Pharmacy Dictionary) / @pharmacydictionary
E-Mail for official and other work - [e-mail]
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPAT

[@ramtashiva]

Thanku ma'am ❤

[@umeshLucknow]

Very nice