Southern and Northern Blotting

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I'm definitely gonna put your name in my end of studies thesis,wait for it,best teacher ever

[@hibaalzain5319]

Agree

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Did you complete your thesis and put his name lol

[@jeppeflner5280]

You are the man, Sir. The amount of work you must have put in to make all these videos is second to none. You are teaching (often times highly) complex matters in a way that makes it very easy to understand for your viewers, and you definitely contribute to a higher educational level among a lot of students, otherwise running their head against the wall, in badly written text books and badly executed lectures at university. Thanks a ton!!

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I don't read my notes anymore. I juts watch your videos and I feel confident with upcoming exams. You're such a great mentor. From the Philippines here, Salamat :-)

[@AKLECTURES]

Awesome! But I think you should still take notes! Something about the process of writing lets your brain understand and remember concepts better.

[@Obamnaz]

Say mahal na mahal kita to him

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no matter how difficult topic it is u never fails to make us understand.. love from Pakistan❤

[@maryc5905]

You make the best biochem videos!! I watched your lectures for my biochem course and now studying for my MCAT! your explanations are x1000 clearer than text books and other resources. thank you so much!

[@AKLECTURES]

Thanks Mary! happy to help :)

[@millssJ]

You take me back man! Been watching you since I first started at uni and now preparing for advanced upcoming exams you're still helping me and lots of students around the world, you're the best!

[@isadora13correa]

This is so helpful for the MCAT, much easier to understand than any other material I've ever used. Thank you SO much for sharing!!

[@onegtive]

nice, I've been trying to wrap my head around these processes forever. I have a much better handle on them now, thanks, you're the man.

[@adrielfrancois6024]

Your videos are the best. Concise well structured really easy to follow. KZbin teacher of the year award should be yours

[@piawendrich9421]

I´m form germany, and I liked the explanation very much. Normaly it´s hard for me to follow english science videos, but you talk very clearly and explain the "stuff" often in differnet way, so that even I can understand these difficult processes. Thanks a lot!

[@stipepavic843]

Epic lecturer !!!! one of the best on youtube !!

[@StudyWithM3E46]

used your vids for MCAT, still using them for medical school. you are amazing

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Your videos are really helpful with my molecular diagnostics class! Our teacher never explains things in detail and it makes it sooo hard to understand anything that she is saying! Thank you for making these! They're a life-saver lol

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I really appreciate your work , such a complex topic I was able to understand after struggling over weeks in just 5 min after watching this in 2x, this is how I have written in my notes,. thanks

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I started med school years ago and then letf 1 year later and start over again this year 2022. Why am I saying this? Because now and then there are themes that its special difficult to me to understand and ALWAYS that I look for te solution on AK LECTURES I find it, but its not just a resolution, its a excellent and simple an clear explanation! I have no words enough to say thank you! You're just AMAZING! I wish one day to be as good teacher as you are! You're literally a inspiration for me! Thank you so much!

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Difficult topics converts to superbly easy only after watching your video......amazing teacher lots of love...

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I love you. You got me an A on my last test and you're a great help for the upcoming one. Thank you so much.

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my lecture notes are so complicated that i nolonger read them just watch your videos. cant wait for my distinction. thanks for making the course easy.

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best lecturer on KZbin. literally saving my life for the MCAT! bless your soul

[@lincolnoliveira3041]

Sheoll

[@Sunshine-zi7oy]

can never find anyone like you you are one of kind🙏🙏

[@martinmurimi4236]

This cleared the confusion i had on RNA and DNA separation issues.Thank you bro

[@mahela1993]

Northern Blotting starts at approximately 7:50

[@Tariqt4]

Thank you so much 👌🏻👌🏻👌🏻

[@madmedicos3759]

Tomorrow I’ll have a biochemistry exam! I’m feeling confident for this topic now. Thanks a lot sir!!

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You are a great teacher!

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thank you so so much. And now I’m subscribed. Been a great help.

[@catherinelee3394]

this was so helpful as review for my test tomorrow. thank you so much!!

[@rpslobo]

Thanks, I've been learning a lot with your videos, it's really great help! Keep up the good work! :)

[@mrsrekima5593]

your explanation is amazing...what a good work ..please continue doing that ...its helps a lot

[@irmasamayoa1964]

I'm so glad that I found this video! That's a very good explanation, thank you so much :D

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It is more easier to understand me to watch your lecture . Thank you for your knowledge sharing

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well explained...thank you so much will be learning from your videos for the whole project ......excellent work.Satisfied

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It was really awesome. you have taught such a hard topic with clear concepts. I have always feared from Genetics but It was very easy to understand. Thanks for this video😊😊😊

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Great, your english and explication is very clear. Thankyou.

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Great lecture, thank u!💖 this lecture exactly help me so much to prepare my uni test🙌

[@arjundang7]

really helpful with the basics . superb AK

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very outstanding and clear presentation, I understood very easily and I thank you very much!! Please add more videos

[@user-vr6ud6rz1c]

The greatest doctor

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genius!!! thanks you speak very well, i´m a latin person and my english is normal jaja.. because the way you speak i could get every word you said!! bravo!

[@ladyRockzETNO92]

Thank you for sharing, you simplify this difficult brochure

[@junczhang]

thank you! great lecture!

[@arminkeshavarz2152]

nicely explained ,thank you my friend for this really nice and useful lecure ,keep up the good work

[@gigi9216]

This is really good, easy to understand. Thank you so much!! You're awesome !! : ))) Keep up the good work :D

[@ocm6382]

Hi there AK lectures, great video - you do a good job explaining the process. I'm wondering - what's the practical application of a southern blot versus a standard PCR amplification & gel electrophoresis genotyping method? Cheers! :)

[@lifelover9989]

Best lecturer ❤ God bless you 😊

[@ganglion0156]

Many thanks, really clear and benefits explanation!

[@saharosamamaray7846]

Thank you from the heart thank you so much ❤️you saved me

[@Luciferin66]

Amazing explanation sir. Well done

[@sruthi.m19]

Very simple! Thanks a lot..clearely understood sir

[@LydiaJohnson-jn6wn]

this was explained perfectly. thanks!!

[@Claudia-xp6ux]

thank u for helping me during this dumb pandemic

[@hibaalzain5319]

God bless you! ❤️

[@meharfatima7731]

Thank uhh sir...u r great..u explain the topic in a very easy way..once again thanks a lot Sir🎉

[@BlacFireSan]

This man is so helpful

[@ayhamabueisheh3772]

you are just really great!

[@ahmadkamal8693]

This is great! along with the rest of your awesome videos :)

[@AKLECTURES]

Ahmed Kamal thank you! :)

[@motivationbyShanjida]

Thank you, Great lecture.

[@AKLECTURES]

Mahbub Rahman you're welcome!

[@kluaywael]

Thank you for sharing information🌻

[@titi14fan]

Thank you , You made it alot easier

[@princeadam5514]

Very simple very easy clear presentation thanks a lot

[@filip4669]

Hey, great vid thank you ! I dont understand why we want to isolate the fragment in the first place if we already have the complementary strand !? Can someone help please :) ? i think im missing something

[@Mariam-iq4if]

You are amazing!!!

[@user-qc3uv8bu8b]

sending love from egypt to your vedios

[@anaherrera4805]

I LOVE YOUR VIDEOS!!!! THK U

[@EstherCamilo]

maybe a silly question: if the sequence is known until the point you can build a complementary sequence of it, what is the point of southern blotting?

[@loubna8308]

good question hm

[@John-hz7jb]

I believe it allows the experimenter to directly isolate the segment of DNA containing the gene from the rest of the DNA (in Southern anyway)

[@beauvoirk8962]

The southern blotting is used to find out whether or not a molecule of dna contains a particular gene ( known)

[@beatricecolombo5475]

The point is to isolate the sequence among a large numer of fragments. Maybe you have a sample of dna from a crime scene and you know just a small part of the killer dna, so southern blot can help you find that little fragment among the sample af dna you found in the scene.

[@calebm9000]

THANK YOU. I was having the same bit of confusion.

[@GoldenSilence333]

Keep up the awesome work!

[@AKLECTURES]

GoldenSilence333 thanks! will do :)

[@bluewarden66]

Just to add, the dna fragments are denaturated with alkali, before their transfer on to a nitrocellulose filter

[@mioulin]

you are the best!

[@hasmiranik1064]

Excellent.

[@claricea5353]

you are the reason i'm passing uni

[@arjundang7]

do you have videos on NGS and other molecular techniques ?

[@KalKiKhabarAaj]

in step 3 after denaturing DNA, why does the denatured DNA attach to the radioactively labeled DNA and not the original complementary strand where it separated from?

[@boobiize]

Thanks u made it alot easier 👍🏼👍🏼👍🏼

[@havvacelik5433]

hi i have a question.. so in the southern blotting part you said you use radioactively heavy phospjoris atoms to label prob dna. Are 'radioactively heavy phosphorus atoms' intercalating agent?

[@annahaener]

that was clearly explained, thanks:)

[@mayzaid437]

thank you thank you and thanks gad to find you your way to explain is remarkable and by the way iam from iraqe and its an honor if you answer me

[@dilo2424able]

Nice Work!!

[@bohoberry0]

Amazing! Thank you! :) 😊

[@amritsharma5373]

Hello sir. I have a query. Since northern bloating doesn't require denaturation (of ss-RNA), do we need to dip the gel inside alkali solution? Or is it necessary for knocking out certain Nitrogen bases for later radioactive labelling in membrane?

[@user-xe5tu5ke8i]

thanxs doc ... from algeria

[@user-vp4pe4ee8b]

Sir ur videos are really helpful, thank you for making them😊 but I would like to tell you one thing that some of ur videos have low voice , please correct it if possible.

[@alonroz1287]

WOW! Thank you

[@krentertainment6518]

Good explanation

[@jjoojjoo20296]

you explain better than my Prof. lecturer

[@arturoliveira3584]

Aula muito boa.

[@ridaimran7565]

If anyone could answer that would be cool. How can we put complimentarty of strand of fragment of interest if we did not know which is our fragment of interest? And if we already know that? What's the point?

[@jacintodelacruz1778]

Regarding the DNA fragments that get transfer into the cellulose sheet, how does DNA denature? For my understanding the DNA in the gel is double-stranded? Maybe the the conditions during the transfer?

[@saunyboy123]

+Jacinto De La Cruz Usually NaOH is used to denature the dsDNA

[@mrglut5560]

Are the ssDNA bound to nitrocellulose permanent or can they be removed?

[@user-dg7vq3ob3g]

Simply you are soooooo great👍 👌 👍 👌

[@halaalabboud480]

You're awesome 👏

[@Volvo990]

Thank you very much for the video, helped a lot. I have a question though, and that is: When does the DNA split into to strands ? Is it when it enter the nitrocellulose membranes ?

[@user-bhavani4]

The first thing that happens in step 2 is the denaturation of the double stranded Dna

[@Chelssums]

thank you!

[@ghl9080]

Thank you so much

[@MEDforyou]

Thank you!!!

[@stavshmueli6932]

I'm quite confused on what Southern blotting is used for. So just to repeat what you've said - In order for us to perform the southern blotting procedure we need to know the DNA sequence of our desired gene in advance (to make a complementary radio-labeled DNA strand). So two questions about that process: 1) So what exactly southern blotting is used for? Is it used for identifying if an organism has our gene or interest and that's it? What else could we possibly use it for other than that? 2) If indeed we use southern blotting for identifying if an organism has our gene or interest in its genome, why would we need to use the electrophoresis gel for? Can't we just denature the DNA strand and throw it with a radio-labeled probe to see if the DNA strand gets attached to the probe and then we can deduce the DNA strand does indeed have our gene of interest? Why would we need to cleave the DNA strand and run the restriction fragments through electrophoresis if all we search for is the gene of interest? Obviously I could be missing something but I'm quite confused on why exactly we need these additional steps as it seems redundant. Please help?

[@ealafabbas9745]

thank you ..l have learned so much..✌✌

[@HanSoloxcs]

That was beautiful :D

[@foxesyse]

you saved my midterm

[@soul1315]

Thank you ...