Splicing

RNA Splicing | group 1 introns | group 2 introns | self splicing | spliceosome |
The transcription of eukaryotic gene results in formation of pre-mRNA. The pre mRNA have coding sequences and non coding sequences. The coding sequences are known as the exons, while the non coding sequence are known as the introns. splicing is a phenomonon in which the introns are removed and the exons are joined togeather to form mature mRNA. the basic mechanism of splicing is simple. the phosphodiester bond between exon and intron at 5' splice site is first broken, then the 3' hydroxyl group of the 5' exon, reacts with the 3' splice site to complete the reaction.

[@sarvsoltani1674]

it helped me in so many ways to better understanding how exactly the splicing takes place! thanks for the great job.

[@rachl8400]

Great video! Super helpful, one thing to note is I believe the correct naming of the G and A nucleosides is "guanosine" and "adenosine" because they are joined with ribose, not on their own.

[@KarthikDisley]

simplest and shortest explanation of all, very helpful

[@okankilic2297]

To add a minor revision, you could substitute "dash" with "prime" word. Thank you !

[@lorrainelolah6841]

hello Okan! can i kindly have any questions on pre- mRNA that you might be studying. i actually approached you since your comment is the most recent.

[@BioTechNomad]

😂😂😂😂 i was thinking the same

[@varnamohan2629]

We use prime

[@memanjoo005]

Best video on splicing

[@joshmemarrynagdey1639]

Helpful video. Thank you

[@chloesmith2607]

Thanks man, I really appreciate this. Have a great day!

[@ummeabiha7323]

this is so far the most simplified yet the best video on the topic!!

[@yarelisrivas-avila7610]

Excellent explanation. Thank you so much!

[@HadasLL]

I'm so happy I found you. You are great!

[@akashsahu6856]

Thank you for your help 🙏🙏

[@extatickiddo5714]

I’ve never heard prime read as dash lol

[@gretabecker2946]

Me neither. Came to the comment section just to see if that's a thing... So, was this by accident or is it, indeed, called 5/3 dash in some other parts of the world?

[@shubhangimorale9684]

@@gretabecker2946 some people call it dash instead of prime which is conceptually wrong. It should always be 5’prime splice site.

[@nadiatumayine7341]

Thank you for letting me know that the dash meant prime, I was on my way to closing the video because I was even more confused :)

[@londonjeremiah8434]

instablaster...

[@bookishmahsa6634]

cool then I'm not alone

[@beatrizvelazquez5430]

Great, just one comment, it's not 5 or 3 "slash" but "prime" (like Amazon, ok bad joke)

[@saimsaim8580]

😒😂😂😂

[@kavyashree8069]

He said 3 dash and 5 dash and not "slash". In most of the colleges and universities they teach us like that

[@anshikajain7556]

It's pronounced prime not slash in biology

[@c0rtikoZteroids1]

@@anshikajain7556 It's common nomenclature; "dash" and "prime" are both accepted.

[@anshikajain7556]

@@c0rtikoZteroids1 ik but i was talking about slash. Thanks btw

[@mmmpr2261]

Bless you,man!saved my day'

[@sumanpanigrahi7275]

Nice...

[@mohanaburman8467]

Loved it ....

[@ummulbanin9845]

Thank you sir for such a simple yet completely clear and understandable lecture

[@manikhalder3520]

loveeeeee you a lottttt god bless youuuuuu

[@liutianchanzwiers3706]

Thank you so much!

[@salmasanad710]

thaaabk you so much i spent three days trying to understand this, fortunately i found this and i understood it quicklyy so now i wish if i have founded this 3 days ago hahaha

[@khoinguyen0210]

Thank you very easy to understand

[@asmita_1999]

Great video

[@paige9274]

great video, explained very clearly thank you

[@alfredomunoz8791]

Also Prokaryotes can't have introns, because they have transcription coupled to translation. They don't have time/space for that, since intron splicing will stop the coupling. Eukaryotes evolved the nucleus, where splicing can be done

[@ganmeii]

they still can have introns, prokaryotes perform self splicing which means it won’t impact translation time wise but it can still happen

[@muhammadabdullah4121]

Why the splicing of intron in transcription occur in eukaryotic not in prokaryotes?

[@manjusamyuktha2013]

Good explanation thank you sir

[@MehwishNaseer-e1t]

You are great bro 👍

[@RAMYAPEDDHURI]

Very nice thank you

[@quickbiochemistrybasics]

Most welcome 😊

[@joyganguly6834]

Really thank you for the video sir, I have never understood Splicing this good before

[@Reah850]

I know I will never ever e-ever give you uuup, and I wanna say thank you in case I don't thank you enouuugh...

[@amnarehman1278]

Waaaaaaao 👍

[@keratin2155]

Your video also need splicing The part where you are speaking nothing need to be spliced😂

[@shamshassan6827]

thank you, you made such a complicated process easy and clear to understand

[@XAbdeVilliers]

Sir which is best book for molecular biology study📚

[@deebos980]

wow nice

[@Alkimachos]

am I the only one who thinks snurp is the cutest name ever?

[@yashusingh2289]

Really helpful..... thank U so much....keep it up......!!❤🤗

[@md.ashrafulkarim6778]

very short and to the point..thank u

[@eisenj21188]

Great video buddy. Thanks!

[@subhasishbhattacharjee8596]

Thank you mam

[@arshbir1539]

Thank you

[@ishanbharti8797]

U5 is attached to both the exons, holding them together

[@bracken7794]

Great explanation, thank you!

[@valentinapaolillo6310]

thanks for the help, great video.

[@apurbamukherjee794]

It's very helpful, thank you for this video

[@manibio2948]

Thanku so much ........

[@suneetsharma907]

Just try to comprehend what she described above is happening in each cell of your body

[@prastutidebi8034]

Really helpful thank you so much

[@mernas1418]

GOATED.

[@ごはんだいすき-d5f]

1:50 ✍️ It helped me a lot, thx🙏

[@sharonbhambal2084]

Now I clearly understand this concept thanks

[@Prettiest_Aayat]

Oh great

[@beatriz4118]

Thanks

[@FreedomForMarkets]

5 dash side and 3 dash side had me. that fail.

[@linagabriel6356]

Thank you so the video.. It was very educating

[@indrajeetsingh-ev3ig]

Is this will going to help in my neet exam it is not clearly taught in our Aakash coaching .... Please help

[@yashasvisharma2394]

This topic is not needed for NEET prep... its high level MS portion

[@pakeezahashraf197]

I did'nt understand my teacher's wording But this vedio help me and make me to understand splicing easily I just love thiss❤

[@DBS608]

Remember no bond brakes, nor new bond form

[@DharitriVishaliniG]

You are a god send. thank you.

[@neethugopal2351]

Thank you.. its very helpful and easy to understand.

[@drajiv1]

Nicely explained 👍

[@shaharbanupp6975]

Tysm,, u really helped me a lot

[@mimineelam1671]

so lariat forms only on group 2?

[@akashbaburaj2349]

can somebody explain what is branching sequence in mRNA and why it is called as branching sequence

[@tanu6464]

☺

[@muhammadtouseeftahir9477]

thank you so much❣

[@rajuislam2490]

Thanks man.. I really get understand by your video a day before my exam.i cann't speak out how am i feeling now!!!!!!

[@zoom-zip3473]

So where does the guanine nucleotide in type 1 introns come from?

[@Sunshine-Muskan]

Great work 👍 very clearly explained

[@Mostafa-hz4kj]

Thanks

[@mitchellhayes2400]

Great video! What is your primary source for this video? Thanks.

[@manikhalder3520]

you are gooooooood

[@evelyneprestrelo3844]

provavelmente é um trabalho de faculdade e eu aqui n ensino médio brasileiro tendo q entender isso... HELP

[@SabrinaScience8]

Thank you so much for this video! I'm a visual and an auditory learner and I thought your explanation was perfect!

[@HarshitKumarSinghAnine]

Thank❤❤❤❤❤❤

[@HAA-wasteAcc]

I thought U1 u2 u4 u5 u6 are snRNPs, not snRNA.

[@maryrosebriones8666]

Hi! I am interested in using your video in my research instruments. May ask I request for your email so I can formally ask permission? Thank you!

[@ankitcreations9678]

💓

[@ahmadansari6829]

Great sir

[@lordhelmchen6539]

Very helpful. The simple explanation of attack here and there from my professor was not cutting it.

[@syafianursyifa5183]

excuse me, Mr. what's book you were used in this video ?

[@funnyvideomarwadi]

Nice information of RNA splicing

[@user8582]

AMAZING

[@apekshadandi7767]

It was quiet effortless to learn splicing from your video. Thanks a ton.

[@razanfeki2909]

you explained something in 7 minutes that my professor couldn't in and hour and a half !!!!

[@Wilderness5283]

Thankk you, it is very informative!

[@ayshamalik2963]

Much helpful💜💜💜

[@josephineobi8089]

Thank you for the video. Please can you help with an explanation why in-vitro splicing reconstituted with U-snRNPs is slower than in-vivo splicing? Thank you.

[@karansahare1349]

Thanks sir

[@240_mandakinisabar4]

Tq so much sir i really appreciate you ❤️

[@anjanasaji6557]

Very nyz clz...easy to study😍❤️

[@mobarkalwahishi3323]

Thank you so much

[@richardshane456]

ty

[@silva0003]

Why are there no videos of examples using a template strand to help us identify introns and exons

[@ВасилийЛ-в7ъ]

То чувство, когда говоришь по-русски а слушаешь по-английски и все понимаешь)

[@melodii8377]

what i dont get it why these parts get transcribed if they are to be cut out.. why not simply not transcribe them and connect the exons like okazaki fragments are connected..

[@mohammadaadam2102]

Helps in giving rise to alternative splicing which in turn facilitates a single gene to produce many proteins

[@lucyxiong7301]

thanks for your help! you're pretty awesome!

[@ganeshgr616]

Great explanation..and . good vedio too.......

[@taniabhadra9313]

What happened to the introns after slicing?

[@mawadaa.nasser651]

Very simple, Thanks