Steps of histological study: fixation

In our new video we discuss the main and most important aspects in fixation. Fixation of histological samples is the first and very important step of histological study. It isnecessary to preserve the native structure of the sample. If the tissue is not fixed, the autolysis will start and the structure of tissue will change because of enzymatic decomposition. All the mistakes made on this step will lead to loss of dignostical value of the sample.
- Our website: en.biovitrum.ru/

[@danieldauber8335]

This was very very well explained. Concise and easy to understand. Well done! 👍🏼

[@merooalasha5115]

Itis really informative video cover most of questions about fixation (why ,how,factors ) , usefull for education

[@merooalasha5115]

I suggest other videos in the same quality about all tissue preperation steps (processing,embedding, microtomy )

[@editingworld461]

Bhokololo

[@user-er7qt2kz7s]

Brief but detailed.👍👍

[@tariqabdulazizsrour5485]

Excellent and very useful for my studies

[@kawtherabbas4058]

Thank you so much for this explanation , it’s great and brief informations .. Allah bless you

[@periyasamymurugesan6282]

Please explain decalcification method

[@muqadershah220]

The video is very informative and interesting. Please mention which pH of neutral buffered formalin solution is better for fixation and how we will adjust that pH of that solution.

[@nazifatasnim2928]

Thanks for the well organized and clear explanation.!! -From Bangladesh.

[@pankajjagdale7057]

very precise and informative.. thank you

[@ayobamidare600]

Thanks for the information. its quite educative. However, if tissue (e.g kidney) has been fixed in 10%neutral buffered formalin for more than a month, can it still be useful for histological evaluation? if yes, what are the likely challenges that may occur? Thank you.

[@beverlywilson-harlacher5467]

CAN YOU BREAK DOWN AGAIN THE MATH THAT IS INVOLVED IN HISTOLGY?

[@obettannamdi9864]

Ur video was of help...thanks

[@carolinanavashernandez2522]

Thank you for the info. It is very useful and easy to understand.

[@د.حسينعليخزعلاختصاصجراحةالعظام]

Nice explanation thank you dear

[@salonigurung1139]

Choden ma'am teaches better 😎

[@sarahe.s7231]

Thank you. I would like to ask. Is the importance of methanol preventing sedimentation of paraformaldehyde..?

[@janarthananjdlab8490]

very use full, simple and easy to follow. please suggest any low price and good quality microtome for small HP start up laboratory

[@BioVitrumEN]

You can use any mechanical rotary microtome, for example, Sakura SRM 200 or SLEE CUT4062.

[@martinmcr83]

You mentioned 24hr fixation time. What temperature do you recommend?

[@ahmeddawood1063]

Biopsy samples do not require 24 hrs fixation , in fact overfixation can be detrimental to tissue analysis

Please, could you clarify the example if 1,5sm = 1,5cm in that way it makes better sense to me.

[@josegabrielvazquez1401]

Thank you for this!

[@spritualworld2667]

Thanks mam

[@goharrehman7710]

what is a special stain for lipids please tell the name

[@nickzsidisin62]

So I am trying to make some tissue samples. The slides look ok around the edges look pretty good but the inside is just all black, is the sample too thick? Or did I fixate it incorrectly?

[@ahmeddawood1063]

Samples too thick

[@Hasan-kl6tq]

Thank you very much

[@Zainabz005]

Thanks

[@timothybanda1766]

What are the 3 types of fixation

[@ek1215]

This is very informative!

[@sajadal_iraqi2411]

wonderful

[@sukhjindersingh7235]

knowledgeable video

[@red4629]

thank you

[@aritraghosh4761]

Mam, can i use dilute papine amylase solution for separating individual myocyte for slide preparation?

[@BioVitrumEN]

Hello! It is better to ask the manufacturer of the reagents that you use.

[@Nora-qu9rq]

thank u so much!

[@anilkushwaha9765]

Dmlt kiye h hindalco industry Ltd power plant Renupower Hospital to work 6year to work good viediv Vaconci he?

[@josephkamalamba]

Was very useful than the

[@mohamedgawada7705]

Who to fixation The Fluid ...?

[@Dr.Amanbansal9730]

great

[@divyalobi2820]

can I use 4% paraformaldehyde for the fixation?

[@BioVitrumEN]

Hello! Paraformaldehyde - is a white sediment, the polymer of formalin. For fixation you should use 10% formaline (which is the same as 4% formaldehyde solution).

[@divyalobi2820]

@@BioVitrumEN thank you

[@divyalobi2820]

If I would have stored in 4% paraformaldehyde, can I transfer it to 4% formaldehyde?

[@BioVitrumEN]

@@divyalobi2820 Paraformaldehyde is not a solution, so the sample can not be stored in it. But anyway, use 4% formaldehyde solution for fixation!

[@Renebaebae607]

@@BioVitrumEN hi we have 37%formaldehyde, is it the same?or should diluted less than 10 times???

[@alirezadehghan7673]

perfect

[@يوسفالحزين-ج2ل]

very god

[@Canalistan]

algorithm trainer

[@farshadnezhadmahdi8129]

Hello and thanks You speak very fast. It is hard to me to understand. Please speak a little slowly

[@nl2457]

In Russia, tissue sample fixes you

[@cornbread415]

What do you mean?

[@mrkillminy]

in iran samples are born fixed

[@bryanu1737]

Polska? Or Russki

[@spritualworld2667]

Special stain video

[@adamdmac3493]

Thank you

[@spritualworld2667]

Special stain video