How to prepare the perfect Gram stain - Gram staining procedure

[@raymundo5200]

I hope everyone enjoys our new Gram Stain tutorial! We put this video together because we saw a great need to train Techs on the proper way to get accurate and consistent results when it comes to performing the all-important Gram Stain. Please let us know if you have any comments...we would love to hear from you! Jay Hardy

[@colinleungcm]

no

[@user-lh6vr6vs4h]

Really helpful for my dermatology Toacs station.Thanks alog.Beautifully explained

[@MelomaniacEarth]

YES FOR SURE. IT WAS WORTH WATCHING.

[@philomenadodoo6228]

yh

[@mostlymicrobes]

Very nicely done and helpful. I do wish the video showed the rinses being done over a staining tray and properly disposed of instead of being washed down a sink!!

[@6ftS]

I felt like I was in a university lecture. brilliant presentation. thank you for explaining it in such detail.

[@HardyDx]

You are welcome. Glad the video was useful and detailed!

[@juliakorolenko8788]

Great video, easy to understand for non-microbiologists!

[@HardyDx]

Great to hear!

[@sukishr2349]

It's very useful for me to do practicals... Thank you so much .... Love from India🇮🇳❤️❤️

[@HardyDx]

Glad it was helpful! Love to see it! Cheers from America!

[@jennyvarshavski3138]

@@HardyDx but if we pour decoloriser more then g postive Bacteria will lose color right ?? So we have to not pour more decoloriser until all the stain comes out ??

[@leannajulien4175]

I'll need to watch this over 3 more times. So much information . Thanks guys

[@HardyDx]

So happy to hear this! We love to be a part of ongoing learning for all our viewers!

[@lojohnson4694]

Great video! Wonderful info and beautiful graphics. Perfect video for either those just learning microbiology & gram staining, or for those looking for a refresher.

[@HardyDx]

Awesome! Thank you!

[@CorruptEggs]

How to prep a Gram stain procedure: Beginning of vid: the atom was theorized by John Dalto...

[@ranyan226]

Sir I am Bangladeshi. I have test sputum for gram stain... Pus cells... ( ++).. Gram positive cocci... Plenty..l want to know about this report...

[@hafizumar5732]

Thanks for practical How much it costs to buy ur products from Pakistan

[@HardyDx]

Hello Hafiz Umar! Thank you for the comment. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our KZbin videos 👍

[@mikevanderman2727]

Love it

[@BriONeil]

I appreciate this video. It will be helpful for my Micro Lab. Thank you. 💗

[@HardyDx]

Glad it was helpful!

[@13anesthesiamithalipawashe57]

Thank you so much sir it is really sooooo good 🤩👍

[@HardyDx]

You are most welcome.

[@kadhimali266]

Thanks, very helpful me to understand gram positive and negative bacteria.

[@HardyDx]

Glad it was helpful!

[@aliza.hyder_]

Wonderful explanation! Thank you

[@HardyDx]

Glad it was helpful!

[@Blessing.x0]

Can absolute alcohol be used to fix the slide?

[@Riftshroud]

Awesome graphics and brilliant explanation! Caught me off guard with the 'culture of service' pun in the promotion D:

[@HardyDx]

Glad you liked it! Service is part of our company culture here at Hardy Diagnostics.

[@virensingh-sw5hk]

Keep it up gentlemen

[@HardyDx]

Thanks, will do.

[@DanTheisen]

Nice video. 👍🏻

[@DanTheisen]

I’ve done a few gram stains in the past and the technique is a little tricky just following a procedure. The video helps to show exactly how it should happen.

[@vanillaaffogato9985]

We're always uses bunsen burner to drying on every step. Will it impact the result?

[@HardyDx]

@vanillaaffogato9985 We recommend methanol because it typically results in a clearer stain. Over-heating of organism on a slide can sometimes cause it to deform or change shape due to heat exposure. Methanol fixed gram positive organisms are also less likely to de-colorize during the staining process.

[@genesantina3000]

Great video, much appreciated.

[@HardyDx]

You are welcome @genesatina3000, we appreciate you too!

[@hansmenares3850]

not a very good technique by the fellow and the method/procedure used its bad. usually you dont attack directly the sample with stain or water or whatever you pour on it. heat its not bad at all, just not direclty for more than 5 sec, like in flames works good enough... air dry could contaminate your sample and/or could have alive bacteria (wich isnt bad at all either but it depends on what you want, generaly dont want alive bacteria so you dont contaminate more tools in your lab).

[@sergiobotello9046]

Well done, well said. Thank you

[@HardyDx]

Thanks for watching!

[@Spandan-Adak]

At @1:25 you've showed that peptidoglycan layer is stained itself. It's not true. It's the cytoplasm that takes up the gram stain.

[@DoniaNabil-li2fg]

Thank you for this interesting video ❤

[@HardyDx]

Glad you enjoyed it!

[@itanruiz7828]

Works very great! Thank you.

[@HardyDx]

You're welcome!

[@daudbaig5109]

gram stain plays vital role in identifying bacterial morphology and the best way to take prudent choice of antibiotics

[@leekspinner]

I assume the videos that used heat to fix the bacteria are representing the old method

[@TheModernVictorian]

Sorry, I couldn't watch all the video because of the music, but otherwise what I did see was informative. That kind of cheesy not-even techno-kinda royalty free noise is just too pervasive for some people to focus on what they actually want to hear.

[@thenone6945]

Quit yapping.

[@sparkleberrypop]

Thank you for this video. It is very informative. I hope you can remove the background music or lower the volume down. Thank you

[@HardyDx]

Thank you for taking the time to watch our video and for sharing your feedback with us. We truly appreciate your input, and we want you to know that your suggestions will definitely be considered in our future video planning. Regarding the background music volume, we understand your concern. Unfortunately, we are unable to make adjustments to previously posted videos without affecting our current viewership and traffic. However, please know that we are continuously striving to improve the quality of our content based on valuable feedback like yours. Once again, thank you for being a loyal viewer, and we hope you continue to find our videos informative and engaging.

[@premkumarbuddavarapu2641]

˚✧₊⁎❝᷀ົ≀ˍ̮ ❝᷀ົ⁎⁺˳✧

[@diomelgella3010]

Atay oy. Ga labad akong ulo

[@kingkura]

Good job.

[@HardyDx]

Many thanks!

[@Kristenesfiles]

thanks!

[@HardyDx]

Our pleasure!

[@mahbubajannat9907]

Thanks sir

[@HardyDx]

You are very welcome. Glad it was helpful.

[@christophercatani8820]

Very thorough and very accurate, bravo Hardy Diagnostics

[@MunyanzizaKevin]

It's amazing

[@leonardodegregorio7383]

What tune is this

[@trygoyoutubechannel399]

How price if you want to buy

[@HardyDx]

Hi, Try & Go KZbin Channel! Thank you for the inquiry. Please navigate to our customer form using this link, hardydiagnostics.com/contact-us/?form_id=4. Select "General Questions" from the drop down menu and fill out the form. We will reach out to you as soon as possible. Until then, enjoy the rest of our KZbin videos 👍

[@sandymaharajan4868]

Which decolourizer use pananum

[@HardyDx]

Hi Sandy! Our manual gram stain kit (Cat. No. GK400A) uses a fast-method, 75% acetone, 25% alcohol ratio. Hope this helps!

[@sandymaharajan4868]

@@HardyDx thanks you 😀

[@pinkpebble_]

makasi bang

[@AtayeroValentina]

Thank you.

[@HardyDx]

You're welcome!

[@kranthiel5034]

Iam pilla premmmmmm💥

[@premkumarbuddavarapu2641]

Wst na kod**ka