H&E Staining

[@MrJazkiller]

the alcohol you're referring is due to "XYLENE removal" process just after deparrafinization...Xylene is miscible w/ absolute alcohol..so use 100% alcohol to remove xylene...then descending concentrations of alcohol..from 90-50% will add-up water from the tissue..."90"% alcohol means there is 10% water in it, 50% alcohol mean 50% alcohol and 50% water . That is where the tissue becomes hydrated..ready for staining...hope u get it.

[@fabianryan9192]

i guess im asking randomly but does anybody know of a way to get back into an Instagram account? I was stupid forgot the account password. I would appreciate any assistance you can give me.

[@fabianryan9192]

@Oliver Trent i really appreciate your reply. I found the site on google and im in the hacking process atm. I see it takes quite some time so I will reply here later when my account password hopefully is recovered.

[@fabianryan9192]

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[@olivertrent9266]

@Fabian Ryan you are welcome :D

[@kikook222]

A lot goes into this. I wonder how someone figured out the alcohol concentration quantities and times needed.

[@BestWinnerone]

It's all based on trial & error.

[@MrJazkiller]

@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated. Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )

[@yasminali7578]

this was the explanation I was looking for, thank you for taking the time to explain this concept.

[@Theonewhoclimbs28]

@DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.

[@cjcares7815]

I'm just being started. Let this video be my reference point! Wish me good luck!

[@ASSHOLELA]

why that guy didnt differentiate or blue?

[@DerSchnurrbart]

I don't think you need to put the slide into the xylene so long when you are deparaffinizing it.

[@tajmuhammad3715]

How much time is minimally required to prepare H and E slide for reporting?

[@rcamobile]

This was...AMAZING!!!! Thanks for the vid!

[@DavidsonLoops]

Thanks, this helped me with my university assignment

[@bellamarie712able]

1 hour in Xylene is a lot isn't it? 0_o We only do 6 minutes total in Florida.

[@hopehope8597]

histobelle may be depending on technician

[@jatnarivas8741]

@@hopehope8597 The tissue doesn't care who the technician is, but maybe the kinds of samples they work with are different

[@ricasaluria8821]

Hello, what are the most widely used fixative in HandE staining method A. Formaldehyde B.zenker formol C. Picric acid D. Osmic acid Please help me answer

[@kushpal3131]

Formaldehyde

[@ManuelGutierrez-zb5xm]

Why the serial dilution in organic solvents?

[@ho-sl1ej]

what is the meaning of two changes of 2 mins each? it just try which put from PBS to absolute EtOH twice?

[@MrJazkiller]

@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.

[@febriyansyahsaputra]

may I have your protocol ? please email me to [e-mail]

[@anirbanmukherjee2811]

check my video on tissue Staining

[@gajendraufat4331]

Sir thankyou apke video se bahot help ho jati samjne main ese hi agge hamare liye video banate rhe

[@gajendraufat4331]

Thankyou khoob khoob thankyou

[@Pistolerong_Pinoy]

That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.

[@MrJazkiller]

@DerSchnurrbart yes i agree.

[@TheRealHarsjan]

I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )

[@simranpangli976]

is this Harris’s haematoxylin ?

[@txdude86]

5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents

[@febriyansyahsaputra]

may I have your protocol ? please email me to [e-mail]

[@zeinabrahnama5749]

Soo helpful thankss

[@AliAzizQualityEducation]

latest video : H&E Staining | Purpose of staining in histopathology | Human Touch in H&E Staining : kzbin.info/www/bejne/jXO4o5ylnbKeia8

[@yashusingh5244]

Thankyou so much sir

[@JoyJaps-g9l]

Fantastic And easier

[@maheshpatidar6125]

Thanku so much

[@TheRealHarsjan]

@MrJazkiller oo ok thanks very much indeed :-)

[@marialima334]

saaai tá tudo errado

[@Azander137]

Is this how babies are made

[@madhanprasathr247]

Nope you are wrong

[@Azander137]

@@madhanprasathr247 Excellent, thank you for the response. Unfortunately I needed the answer to this nearly a decade ago...

[@madhanprasathr247]

@@Azander137 sry bruh before a decade i was just a school kiddo 😂

[@ذيبالشمال-ث9ب]

@@Azander137 n this medicine how we get sections tissue

[@ricasaluria8821]

Hello, what are the most widely used fixative in HandE staining method A. Formaldehyde B.zenker formol C. Picric acid D. Osmic acid Please help me answer

[@LAHM-TUBE]

I don't know the most widely but I used Formaldehyde to fixed gills and stomach of fish.

[@tajmuhammad3715]

Formaldehyde