Storing mushroom cultures long term with slants, best practices storing slants and culture care

[@stricnine69]

Why do agar slants last longer than just an agar petri dish?

[@SK-ql5bx]

Because of the depth of the agar and prevents it from drying out.

[@eliasdskill5008]

Ty for helping my journey!

[@EricRohlfs]

Perfect timing, was planning on making my first slants this weekend. Was curious about using parafilm on the lids. Parafilm is so satisfying to stretch around petri dishes

[@DracoDikaryon]

Until you stretch it too far and it rips I suck with parafilm lol

[@MartynasShnaresys]

It makes some mastery using parafilm fast and ensure longevity. It has a few resistance levels, first it's less springy, then suddenly loosens. That's the perfect moment to stick and also ruin it by completely ripping or overstretching enough to get holes there later by itself. Cling film works quite great too (cut roll with a knife into smaller rolls). Just don't mistake for a saran wrap, it's not breathable.

[@AdmiralAckbar666]

Can’t wait to try your lion’s mane culture! Mush love 🔥🙏💨

[@BoroBootBoy]

Good stuff. Cheers Gary.

[@indemwildes9426]

I appreciate you guys and what you do Mush love

[@SERGEYFT]

Hello, my name is Sergey. Tell me, do you have experience storing mycelium on slanted agar in a freezer (-20 C)? My experience can be said to be 50/50. Half came to life within a week and the other half did not. I began to suspect that half of the mycelium was lost not due to freezing, but due to drying out. What experience do you have in this matter? The result is the same both on grain and on agar.

[@shakhismail7258]

please tell me in my country there is no place to buy mycelium, I once bought it from another country, every time it is very inconvenient to buy from another country, what do they do to me please tell me I grow oyster mushrooms, is it possible to somehow store the culture forever and use it? sometimes cloned a mushroom after 2 3 times the strain degraded

[@jbb3141]

Hi Gary, great video! One question. Most of the centrifuge tube lids are HDPE and not PP. Have you had any issues, like melting, when autoclaving them with the tubes?

[@linabelarouci9715]

Great video ! Thanks 🙏

[@NurikNurova]

please tell me how long you can store your favorite strain of mushrooms, you can somehow store it forever so that I have this culture all the time

[@LoganAddisMusic]

Hey Gary! Great video as always! I'm going to build a flow hood soon, I'm wondering if yours is made of two stacked filters and it ok to have a seam in the middle? I got a good deal on two filters so I thought I would try it

[@FreshfromtheFarmFungi]

this one is a single unit with two filters (it’s cheaper to replace two smaller than one large one) but I think stacking would also work

[@LoganAddisMusic]

@@FreshfromtheFarmFungi thanks for the reply! It helps a lot. I was wondering if a seam would mess with the flow but apparently not since yours is proof of that! Have a great day!

[@Ruben-zu2wc]

Hi great video, im wondering if you can store mycelium in a vacuum sealed package?

[@FreshfromtheFarmFungi]

yes if it has the correct nutrients, water content and is sterile/kept from freezing or high temps and uv exposure 👍

[@rancidhotmail]

how long can mycelium be stored at 40 degrees? I placed petri dishes in my fridge and found that none of them were usable after around 1 year. When I took them out and tried to continue working with them on agar plates, the majority did not grow on agar. Some mycelium did spread a little, but it barely colonized the agar plates. Any advice on what went wrong or why I couldnt revive the mycelium?

[@Eu4ia_]

You want to refrigerate once the plate is colonized

[@irate74]

Do you prefer to keep your pink oyster LC’s in fridge or room temp?

[@FreshfromtheFarmFungi]

I store it in slants in a closet next to my utilities so it’s like 50F in the summer and 40F in winter. I have kept in the fridge for a while but sometimes I worry it could freeze

[@hdb-r2l]

Hey Gary, I just made some transfers from slants to plates. Slants were stored for around 4-5 months in conical tubes in refrigerator, is it normal for the mycelium from the slants to be a little slow to get going when put back to plates to grow again?

[@FreshfromtheFarmFungi]

yes I usually take them out at room temp for a day or so and then transfer - this can help a little

[@mintislad3900]

@@FreshfromtheFarmFungi hey bro could you go in depth about genarations of mycelium how long a phenotype lasts and how often you have to start from spores again thanks bro

[@mchaney2003]

@@mintislad3900 +1 for a video on how often you need to return to spore, and the lifecycle, etc. Can you just clone or g2g forever? I've heard folks say theyve used the same culture for nearly a decade without returning to spore, others saying senescence sets in if you dont return to spore (or even if you do). I'd love to hear your take on it @Fresh from the Farm Fungi

[@imanderdumme8706]

🙏 thanks

[@Sssanbo]

Do you change you recipe for slanyts? I’ve heard less nutrients

[@FreshfromtheFarmFungi]

I have and haven’t noticed a difference - I think changing up the food source is more important than the concentration but this is just a hunch.

[@rootspherefarms5104]

Why can't you effectively store them in room temperature? Since growth is stalled, how exactly does temperature drop help anything?

[@FreshfromtheFarmFungi]

slows down metabolism

[@rootspherefarms5104]

@@FreshfromtheFarmFungi I get that, but doesn't metabolism only matter if cells are dividing? I keep my slants in room temperature and they effectively stall and go dormant for years, part of me wonders if refrigerating really is much benefit

[@ShawnTheRazor]

@@rootspherefarms5104 I've got a fully colonized petri dish with micropore tape around it. How long you recon this will stay viable at room temperature? Debating transferring to a slant soon.

[@gansaifock3476]

How long would you store the culture for using the refrigeration method?

[@FreshfromtheFarmFungi]

I usually rotate every 6 months but they can last for years

[@gansaifock3476]

@@FreshfromtheFarmFungi Thanks for the reply, I'm a grower from Malaysia and it'a difficult to get good commercial strains here, so I have been trying to figure out how to make my culture to last as long as possible.

[@thematrixcentre]

At whar temp do you keep the incubator?

[@FreshfromtheFarmFungi]

72F (+/- 2 degrees or so)

[@thematrixcentre]

@@FreshfromtheFarmFungi thanks.

[@RubberDuckStyle]

👍👍👍👍👍👊

[@pauloantunes8826]

Great video!! You make mother cultures from spores or clones of mushrooms? How do you manage cultures for production? Can you make a video about your process?

[@FreshfromtheFarmFungi]

Thanks a lot of our cultures are from spores but some are from clones too - I usually alternate between pda and mea and start from slants and go to LC and test on agar, use the plates that are clean and repeat.

[@hoodyk7342]

Stop saying fam and im all on board for this channel lol. Very useful info. Couple terms i don't know but very helpful.

[@FreshfromtheFarmFungi]

taken into consideration

[@summerkid2503]

Fam.

[@Phoenix-One0922]

Too many important points missed and not mentioned here. Philly G.T. explains much better than most, including this channel... He never leaves anything out we might be questioning. P.G.T. Never sticks his face into the camera and instead, does what he's talking about with live examples... This guy here like many others (like Willy, the "hand flapper") they're all about "me time" being 'on camera' and it's just talk, talk, talk.... How boring... Back to P.G.T.!!