Confocal microscopy principle tutorial

Confocal microscopy principle tutorial - This microscopy lecture is going to explain the Confocal microscopy principle and working in details. Confocal microscope is widely utilized in the imaging of small specimens. Confocal microscopy is a type of light microscopy that is used to visualize biological samples. This video explains the following topics in detail:
1. Confocal microscopy principle
2. Confocal microscopy working
3. Confocal microscope structure and set up
4. Confocal microscope principle and working
5. Confocal microscope uses
6. Applications of confocal microscopy.
So, if you want to know more about the confocal microscope and confocal microscopy techniques working principle in detail, stay tuned to this microscopy lecture.
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Thank you for watching the microscopy lecture on Confocal microscopy and the confocal microscope working principle.

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[@MN-mj9ok]

Thank you for the very helpful video. I have two questions.1. Why do we limit to 100µm for penetration depth by confocal microscopy? 2. Do we get 2D image with 3D feeling to it in confocal microscopy or exactly 3D image?

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Very clear explanation

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[@srikantadash4603]

I liked ur video because the Way of explaining is awesome.... It's excellent suman bro... But but but If u provide a computer generated picture of confocal microscope arrangement and how the specimens are visualized in confocal microscope. It would be much better sir. Hope u meaning my statement in a right way. Thank you sir... Lots of love ❤sir.

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[@arpitahalder5005]

at 8:41 you said in case of confocal emission coming out from unwanted site is cut out and that coming from nucleus is received, how?could u explain

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Sir, how pinhole present at detector can differentiate non specific and specific flouresce as the similar florescence are emitted by flourochrome

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Thanks a lot! I got a question: what are the qualitative and quantitative limits of this method? Can you recommend me an understandable paper on this?

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Is confocal microscopy is used to study living cell?

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So, before taking image first the nucleus should be flourescence active.

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confocal microscopy or confocal electron microscopy m kya difference h?

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but I have a question: why a confocal microscope has much more spectral flexibility than a spinning disc microscope?

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Why is it called confocal microscopy?

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