Directional cloning

[@user-lk1cl6ev7g]

I have watched a lot of educational videos in university but this one really stood out to me and I had to comment. I was so confused by a practice question and could not find anything online to help me understand. Of course Shomu’s Bio would have a video explaining it in such a simple way!! Thank you so much I am so grateful for you

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Stay tuned for more

[@newestgirlz]

Amazing explanation! Many websites I went to failed to mention the fact that the portion of the vector between HindIII and KnpI is removed and replaced by the target sequence. Thank you!

[@jackhaberski2428]

Thank you thank you thank you! Not sure I would have made it through my assignment without this video, you're the man!

[@shomusbiologyofficial]

You're welcome

[@shurok84]

Thank you, this really really helped. You're an amazing teacher, you simplify every thing in an incredible way!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@johntindell9591]

Wow, stunningly intelligible explanation. I was struggling with this idea before I listened to your video.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@SanilNadar]

Thank you very much for your efforts. The explanations are crystal clear. We really appreciate your efforts.

[@shomusbiologyofficial]

You're welcome

[@kenichikender5178]

Mate! that was easy to understand. you are a bloody legend

[@MohammedAli-yd2so]

Thank you Shomus for your lectures. they are very useful.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures.

[@biochemisthana2839]

Shomu's Biology very very simple explanation thank you very much

[@fortitude_ra_kolahal]

What is the probability of insertion of the same fragmented DNA ( created by 2 restriction enzyme) of the vector into itself. ( In other words, how come the restriction digested fragment from the vector not compete with the digested fragment of the cDNA to get incorporated into the vector itself)@@shomusbiologyofficial

[@macielrodriguez6697]

Thank you so much shomu! As always, your lectures are amazingly clear!

[@AqsaRasheed-z6j]

lots of respect and love from 🇵🇰 amazing teacher keep it up 💗

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@AqsaRasheed-z6j]

@@shomusbiologyofficial u deserve all these appreciation 👍

[@harshpatel6445]

What a great way to Explain the scenario Awesome sir really liked it >3

[@divyasharma7321]

Thank you sir for making such topics .this helps lot

[@shomusbiologyofficial]

You're welcome

[@Ugandan4evr]

Excellent video. Your explanation was very clear. Thank you

[@norhazlinjusoh5297]

awsome lectures with great explaination...thanks

[@shomusbiologyofficial]

You're welcome

[@SohaNoseir]

Thanks! you are pretty talented at explaining things

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@lesliehinojosa6517]

Thank you so much, I needed such a visual explanation

[@shomusbiologyofficial]

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[@mayukhroy5393]

Your explanation was very helpful ,sir.

[@shomusbiologyofficial]

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[@lanoanhduongthi3536]

Thank you your explaining, your videos very useful for me and also improve my English language

[@shomusbiologyofficial]

Thanks

[@sathyasiva209]

the explanation is so clear, thank you

[@shomusbiologyofficial]

Thank you

[@fatimaea1462]

You are an incredible teacher! Thank you

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@sara97979]

You are an awesome teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@saro4761]

You are an AWESOME teacher man!!!! Thanks so much...

[@sylviachong5988]

THANK YOU I NEEDED THIS

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@112287hihello]

It's really good video. I learned a lot from you. Thank you

[@loressefe313]

Thank you very helful i understood it from you better than our teacher!

[@shomusbiologyofficial]

+Loressef E yes you can

[@citrustiger_]

You helped me so much. Thank you shomus!

[@rahisuddinaligarh]

Very nice explanation of PCR cloning

[@anniquekathompson3116]

Brilliant. You deserve many lollipops.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@saiprasadamanikonda7530]

This was an awesome explanation!! Thank you so much!

[@shomusbiologyofficial]

You're welcome

[@ayaqz3144]

love your explanation .thank you

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@mjgerhart6014]

thank you so much, this is an awesome video !

[@shomusbiologyofficial]

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[@geethuaji9823]

Thnk u shomu..u r video very helpful

[@cutie7794]

Very very helpful! Thank you 👌🏻

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@apurva2171]

Such nice explanation

[@shomusbiologyofficial]

Thank you

[@balasubramaniamthuvaraki3908]

Simple, Informative. Thank you so much

[@shomusbiologyofficial]

You're welcome

[@yiyasyomi]

Thank you, now is very clear, you´re awesome.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@mokakabyengwakwana1101]

this is so helpful and more clear. Can I achieve directional cloning by adding same restriction enzyme sites on both ends of my gene?

[@quyoshniki]

i guess no

[@Sara-fi5nt]

thanx, it very useful. I have a question, if the DNA insert is flipped during ligating it in the clone, how this is going to change the ORF? is int there a transcription specific site to indicate the start point?

[@cryptomarketandswap2517]

hi but what about the part deleted from vector it can again ligate whit vector becuse thay are match how we know the gene was ligated or the part of vector again ligted?

[@abidxd100]

Super informative, Thank you!

[@shomusbiologyofficial]

You're welcome

[@aviatrixspouse]

plz make video on how to determine orientation of insert in vector by pcr

[@suchetanasaha6942]

for directional cloning , only sticky ends are preferred or blunt ends can also arise and are preferred?

[@sanar3246]

thanks mate..that made much more sense..Legend!!!!

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@inshaamin1525]

AWESOME.. HELPED a lot

[@leeann8105]

Thank you!! This really helped :)

[@imrocknreeling]

Vola! That was nicely taught.

[@mehulibandyopadhyay5756]

Sir can you please suggest a book of biotechnology for semester 6 , cu?

[@hindb5758]

Thank you ! very clear now

[@bh5169]

Thank you!

[@shomusbiologyofficial]

You're welcome

[@rozhingh9345]

thank you! helped a lot .

[@shreyadas3278]

Thank you!!

[@shomusbiologyofficial]

You're welcome

[@thegreatangmoh]

Good job sir

[@chavadhariratilal2860]

Thank u so much sir

[@mahyarsh87]

this is directional cloning. is there any difference with directional cDNA cloning??

[@boatraft]

Wouldn't directional cDNA simply be the same process, with the specification that the DNA inserted would be a cDNA? It would be important to use cDNA as opposed to regular ol' DNA, any time you were cloning Eukaryotic genetic material into a bacterial plasmid. Bacteria don't have the mechanisms for dealing with introns, and any DNA you cloned which included introns would result in the production of dysfunctional proteins.

[@welcometosagnicksworld3894]

love you dada

[@shomusbiologyofficial]

Thank you

[@ning5961]

can anyone tell me abt the unidirectional cloning

[@shomusbiologyofficial]

In a separate video

[@ning5961]

Tq sir for replying my comments ,if possible sir can you please make a video about unidirectional cloning sir 🙏

[@ning5961]

As well as heterologous cloning and homologous cloning sir 🙏

[@kotikotordia7496]

Thanks a bunch

[@shashikalat1363]

Very nice

[@salmaa2870]

thank you

[@nawrasal-hasanat3643]

amazing Thank you

[@shomusbiologyofficial]

You're welcome

[@limjiaan915]

Thank you so much