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Surface plasmon resonance is an optical based technique, used to detect interaction between molecules, in real time. Surface plasmon resonance occurs when a metal is placed on the top of surface, where light is undergoing total internal reflection. A group of electrons in the metal absorbs the energy from the light undergoing total internal reflection. As a result of energy absorption, the group of electrons starts oscillating. These group of electrons is known as plasmon and the phenomenon is known as surface plasmon resonance. Because of SPR, a dark zone appears in the refracted light.