[TALK 5] Single Molecule Techniques - Chris Batters

Single Molecule Techniques
Speaker: Chris Batters, MRC Laboratory of Molecular Biology, UK
2024 Introduction to Biophysical Techniques
The LMB Biophysics Facility houses a wide range of state-of-the-art and in-house built instruments that enable the molecular characterisation of macromolecular size, shape, mass, stability, binding affinity, dynamics and interaction kinetics.
The facility is primarily intended for hands-on research by students, postdocs, and group leaders, who prepare samples and make measurements. Biophysics supports users in advice on experimental techniques and results, training and in collaborative research.
In this video Chris explains why single molecule measurement should be untaken, the practicalities involved and several applications using the current single-molecule set-ups in the LMB including FRET and fluctuation correlation spectroscopy (FCS). In the second part of the video, he explains the physics of optical tweezers and how they can be used to measure forces of biomolecules. He shows examples of how the current system has been used in LMB projects. In the last part of the talk, he details how mass photometry, a single molecule light scattering technique, is used to measure masses of biological samples in solution.
The Biophysics Facility is headed by Stephen McLaughlin, supported by Chris Johnson and Chris Batters.
Read more about the facility here: www2.mrc-lmb.cam.ac.uk/resear...
You can find more videos from the LMB 2024 Biophysics Techniques Lecture series here:
• Biophysical Techniques...
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