Tetrad Analysis-Part I-Ordered Tetrad Analysis

Tetrad analysis is used for gene mapping in case of haploid system. In tetrad analysis, the gene mapping is done by measuring the distance of gene of interest from a centromere. Centromere is a great reference point because it does not show crossing over and shift in position. Once we established distance of gene of interest then we can measure distance of other genes form the gene of interest.
Tetrads are of two types:
1. Ordered Tetrad Analysis: in which the spores are arranged according to the order in meiotic division. This is observed in Neurospora.
2. Unordered Tetrad Analysis: here the order or arrangement is not specific. This is observed in yeast.
Neurospora has an interesting reproductive patter. It undergoes two rounds of meiotic divisions followed by a single round of mitosis and this results in production of 8 haploid asci spores at the end of reproductive cycle whereas yeast stops after meiotic division and produces four spores at the end.
At 15:29 an example is given on how to do the calculation and find out recombination frequency.
Formula for finding Recombination Frequency = Number of cross over product / Total number of offsprings x 100
Remember to divide the RF value by two to half the frequency.
Unordered Tetrad Analysis: nowiknow.co.in/...
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Let's Learn Together 😊

[@NowIKnowbyRupalGogia]

Hey there amazing people. I hope this video helps you get an idea about ordered tetrad analysis. TBH gene mapping use to be a bit scary topic for me but once I understood the mechanism, it became easy and I loved doing the recombination frequency problems. What about you guys, do you like gene mapping? You can watch Unordered tetrad analysis here: nowiknow.co.in/2017/02/16/unordered-tetrad-analysis/ and watch Three point cross here: nowiknow.co.in/2017/02/16/three-point-cross-linkage-mapping/

[@asawariahire8549]

The easiest way to understand the tetrad analysis. Thank you.

[@gayatriathavale961]

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[@NowIKnowbyRupalGogia]

Thank you so much for sharing your feedback. Your appreciation means a lot 😇🙏🏻

[@happygill3943]

Best video!!!! Solved all my doubts in a simple and easy manner, can't thank you enough for your efforts.

[@patrixiaaron740]

Thank you for this, I was having trouble understanding why does the percentage have to be divided by two, but watching this video helped me figure it out.

[@blackswan6199]

Thank you so much ma'am, you have explained it so well!

[@sreenamuduri5940]

Spending 24min for tetrad analysis is really Worth. And you are fantastic in explaining..there is no such point where I get confused.such a crystal clear explanation madam 👏👏👏👏

[@NowIKnowbyRupalGogia]

Thank you so much for your feedback. I am glad that video was helpful 😇🙏🏻

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[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@sathmidinanja]

Very clear and simple explanation. Thank you

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maam very helpful video... you start from basic ...helps a lot

[@NowIKnowbyRupalGogia]

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Thank you so much for the feedback.. I am glad it helped 😊

[@britt8870]

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Thank you, this makes so much sense now!!!

[@NowIKnowbyRupalGogia]

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[@angelinalutsyk9950]

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[@shuvodip95]

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[@NowIKnowbyRupalGogia]

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Thank you ma'am for making it simple.....

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[@NowIKnowbyRupalGogia]

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[@saishaprabhukholkar7583]

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[@bongcherk6447]

Thank you so much... I could understand you better than my professor

[@NowIKnowbyRupalGogia]

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Know I know. :) Thank you for explaining this so well!

[@NowIKnowbyRupalGogia]

Yeyiii 😀 I am glad it helped 😇

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So nice of you.. Thank you 😊

[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@janavi07]

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[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@avanraws77]

EXCELLENT!

[@NowIKnowbyRupalGogia]

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[@harramkhawaja6396]

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[@NowIKnowbyRupalGogia]

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[@jeliyaashish]

nice expalination

[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

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[@bsupriya5300]

Thank you Mam

[@NowIKnowbyRupalGogia]

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[@manikantakolasani8440]

U r really good Can u maje solns to the problems on lod score in genetics

[@syedshahidmusvi3287]

Good one. Should not we use 1/2 in formula during RF calculation, I think that will be easy.

[@NowIKnowbyRupalGogia]

Yes you could do that. For the purpose of explanation I am showing it after calculating RF so that it is not confusing :)

[@SafiyaAafreen2711]

lifesaver. thank you

[@NowIKnowbyRupalGogia]

You are very welcome. I am glad it helped 😊

[@themolecularbiologist..5812]

Thnan you mam....

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[@vikramporia6423]

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[@NowIKnowbyRupalGogia]

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[@priyankapriyadarshini6223]

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[@NowIKnowbyRupalGogia]

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[@febinapeter8782]

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[@anupmallick81]

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[@NowIKnowbyRupalGogia]

You are welcome. Epistasis video will be uploaded next 👍🏻

[@NowIKnowbyRupalGogia]

Follow this link kzbin.info/www/bejne/h528aXylodain8U for video on Epistasis.

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[@NowIKnowbyRupalGogia]

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[@aishafehmin2247]

Thanku mam♥️

[@NowIKnowbyRupalGogia]

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[@NowIKnowbyRupalGogia]

Thank you so much for such complement, comments like this keeps me motivated. Thank you for sharing your feedback 😊

[@goppotube]

please put a video on lod score analysis

[@NowIKnowbyRupalGogia]

Will try to make one.

[@anupmallick81]

mam the video is very nice 😊...i understand very well......but only 1 pblm the video is not clear 😢

[@NowIKnowbyRupalGogia]

Thank you. When you play the video, go to settings and in quality select 480p or more that should help 👍🏻

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[@laiqurpathan5620]

A lect on pseudoalleles plss

[@yangskityangskit7161]

very helpful mam 👍

[@NowIKnowbyRupalGogia]

Thank you very much.

[@jaykrishnabehera257]

Bit of a confusion at the RF value being divided by 2. Why don't we do that in diploid cases or previous cases of recombination???

[@daniellemeans5965]

i need unordered asap please lol

[@NowIKnowbyRupalGogia]

Follow this link for unordered tetrad analysis kzbin.info/www/bejne/iJCmZ3Scrbubi7M

[@nahnahnah30]

thank you!

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[@singaravels174]

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[@NowIKnowbyRupalGogia]

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