The Basic Principle of Chromatography and the Different Types of Chromatography

  Рет қаралды 66,835

Biomedical and Biological Sciences

Biomedical and Biological Sciences

Күн бұрын

This video is an explanation about the principle of chromatography

Пікірлер: 34
@StreetRacer-m3l
@StreetRacer-m3l 4 ай бұрын
1. The sample is added on the stationary phase 2. The mobile phase is added 3. The mobile phases moves through the stationary phase 4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases. THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊 But as soon as the mobile phase is added, the mobile phase started to run fast pulling the components in the sample along the way through stationary phase👯‍♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted stoppedsomewherein between. But sadly there were nobody with him when he ended the race and looked back. Poor mobile phase 😢
@Tinugaaduvlogs
@Tinugaaduvlogs 4 жыл бұрын
Praise be to LORD JESUS Thank you..good explanation,very helpful......
@thomastimmers9205
@thomastimmers9205 4 жыл бұрын
Very clear video and explainations, thank's !
@ridwansalifu6987
@ridwansalifu6987 Ай бұрын
Please your English ....
@adarshjha1999
@adarshjha1999 6 жыл бұрын
In paper chromatography why we have to remove the stationary phase before the whole sample pass through the column?
@biomedicalandbiologicalsci4989
@biomedicalandbiologicalsci4989 6 жыл бұрын
The column ? the mobile phase will migrate through the paper and then you need to stop the migration at a certain point to be able to calculate the retention factor ..
@SherKhan-jc7yd
@SherKhan-jc7yd 3 жыл бұрын
Kindly review the stationary and mobile phases
@amiralangsa1583
@amiralangsa1583 Жыл бұрын
ndak jelas!!!!
@susmita93
@susmita93 6 жыл бұрын
i want to know about retention time void volume etc in column chromatography
@terranceharper6193
@terranceharper6193 3 жыл бұрын
A trick : watch series on flixzone. Been using it for watching loads of movies during the lockdown.
@milojayson2542
@milojayson2542 3 жыл бұрын
@Terrance Harper definitely, I've been watching on Flixzone for since december myself :D
@zahidhasan5083
@zahidhasan5083 Жыл бұрын
Very good explanation. Anyone, even though he/she doesn’t have science background, will understand you concept
@obsaaabdiisaa9745
@obsaaabdiisaa9745 2 жыл бұрын
Hi, Have fine? I wish to you that you have a great day. please explain all types of all chromatography
@bijayanag6500
@bijayanag6500 2 жыл бұрын
Can you plz make a video on purification by affinity purification.Your videos are very helpfull.
@Justadamson1
@Justadamson1 2 жыл бұрын
Your teaching is good but your accent is confusing
@riteshyadav3302
@riteshyadav3302 5 жыл бұрын
Nice concept
@isabelladebrisac3055
@isabelladebrisac3055 3 жыл бұрын
Could you please tell me about the controlled variables in Chromatography?
@mohammedburhanuddin303
@mohammedburhanuddin303 5 жыл бұрын
Your voice is quite awesome
@mohamedsakr4437
@mohamedsakr4437 3 жыл бұрын
Haha simp
@ramchandrasuthar2848
@ramchandrasuthar2848 7 жыл бұрын
Very nice analysis
@leticiacavalcante2591
@leticiacavalcante2591 6 жыл бұрын
Thank you so much!!!!!!
@АннаЗагребельна-ф7ю
@АннаЗагребельна-ф7ю 4 жыл бұрын
You say at 16:16 that mobile phase holds our sample inside, but as you explained later in both paper and column chromtography the stationary phase holds the sample at first. Why is this inconformity? Explain please
@StreetRacer-m3l
@StreetRacer-m3l 4 ай бұрын
1. The sample is added on the stationary phase 2. The mobile phase is added 3. The mobile phases moves through the stationary phase 4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases. THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊 But as soon as the mobile phase is added, the mobile phase started to run fast and pull the components in the sample along the way through stationary phase👯‍♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted. But sadly there were nobody with him when he ended the race and looked back.😢
@Stars-uz2hp
@Stars-uz2hp 2 жыл бұрын
اكو عرب بلطياره🙂😂
@pasulasanthoshi9489
@pasulasanthoshi9489 2 жыл бұрын
Thank you🙏
@bh5169
@bh5169 3 жыл бұрын
Thank you!
@devendrachaudhary4544
@devendrachaudhary4544 4 жыл бұрын
Angrej khi ki
@SOFTPOISON
@SOFTPOISON 5 жыл бұрын
nice explanation
@joybulan1800
@joybulan1800 5 жыл бұрын
So helpful
@shivugowda1185
@shivugowda1185 4 жыл бұрын
Suscribe me bro
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