🔬 The benefits of magnification beyond resolution | Amateur Microscopy

[@geoffgeoff143]

Very good point

[@LeakyBucketHandle]

Very informative, I've indirectly noticed the relationship between objective magnification and depth of field and this confirmed my suspicion.

[@mosfear]

Thanks for posting this. From what you say, it would make a lot of sense to use a higher power eyepiece and lower power objective when viewing objects on concave slides.

[@robertYTB78g]

Thanks for posting. Another thing that I really like about using a higher magnification eyepiece is that I can keep the objective a greater working distance away from thicker specimens. On my microscope I only have 4 objectives - 4x, 10x, 40x and 100x, and the distance from the objective to the specimen when moving from 10x to 40x is greatly reduced. More than once I have accidentally put the 40x into the gunk! Staying on the 10x but increasing the eyepiece power is a way better solution for me in this circumstance.

[@balancebreaker6885]

Is there great of a difference when using a higher magnification eyepiece instead of a high magnification objective?

[@LostBeetle]

I swapped my 10x for a 20x and I find myself using it waaaay more than I used the 10x. 10x was too close for scanning the slide, and too far for any close observations. I also swapped my 100x oil for a 60x dry for obvious reasons.

[@thomasschwarz1973]

Interesting details on optics... Thanks!

[@xnamkcor]

To avoid the "reduction of light", would it be feasible to use a digital camera to zoom even further? Once digitised, You can't get any new "information" beyond what was captured, but enlarging for ease of viewing is very possible. As a person, with visual limitations, looking into a 10x eyepiece may produce less for me to see than even the limited digital information captured by the camera that I can view. Even though I've been told optical zooming is always best(and I do still believe this to a certain degree). If there is a limit to the optics and light, but a camera can pick up about 25x as much fine detail as my eye. It seems like I can effectively zoom at least 10x via a camera.

[@hashif619]

*Can you review Olympus DSX 1000 model digital microscope please*

[@jors800]

What effect does a 2x or 3x Barlow lens have on the image quality?

[@khalidimran2711]

I have a question regarding sanitization of microscope during covid-19. My class consists of 40 students and we want to observe some slides under the microscope but there are only a very few microscopes available. What is the best way to use the microscope in this case? Should we sanitize with 70% ethanol after every use ?

[@shukriali5152]

Thanks teacher

[@jimzielinski946]

In the last couple of years, and after a few eye surgeries, I've found that using slightly higher eyepiece magnification helps me compensate for the lower resolution of my old eyes. I rather do that then think about giving up the hobby.

[@xnamkcor]

I've heard not to go over 1000x. What if I have a 10x eyepiece and a 75x objective? is that any better than a 25x eyepiece and a 30x objective?

[@simonachmueller]

I have another question, in what case it does make sense to use a low power eyepiece (e.g. 5x) compared to normal 10x?

[@MicrobehunterMicroscopy]

To get a wider field of view. You can see more of the specimen to get a better overview and orientation and sometimes you do not need a higher magnification.

[@trafalguarnerilaw7588]

Hello mister !! I'm studying biology, and have a lot of histologic aspects in my university. It interests me a lot and I would like to learn it by myself too, using a microscope, and slicing some plants to study their anatomy and learn to find some caracteristics they have. This will require for me to get a microscope. Found a lot, some which has a very impirtant magnification : 2 500 X. I'm pretty sure it'll be impossible even with this kind of microscope to study any virus : ~ 100 nm, but I'd like the higher magnification as possible to make it possible for me to study the more different species I could ! Would you have some advices to help me to find a good microscope, for a student (L2) ? Btw : sorry for my english, I'm french and try to do my best to be clear and as correct as possible in my sentences ^^'

[@MicrobehunterMicroscopy]

Plant histology does not need much magnification. Maximum magnification is 1000x (100x objective and 10x eyepiece). You can get higher magnifications by changing the eyepieces (cheap, magnification is not a quality measure). Have a look at this microscope (or similar): kzbin.info/www/bejne/bIG6fo2Em56npcU

[@majstor76]

Hm, what about low power objective and digital magnification, could that be ok?

[@MicrobehunterMicroscopy]

Here you might also reach the resolution limit of the camera. Depends how much you mangify.

[@ansariih]

This is a very interesting information, thanks!

[@abdelhakmeddoc5568]

Hi Oliver. Is the microscope camera you are using on that microscope (the one we see here in this video) is good for video recording? I want to use a similar one and record videos than edit them and display them on large 4k screen (65")

[@MicrobehunterMicroscopy]

It's this one: kzbin.info/www/bejne/hGKlf3SajdpropI The USB 2 connection is too slow to record high resolution video at a fast frame rate. For video, I would use a camera that does not rely on a USB 2 connection.

[@scottmiller4141]

Another consequence of using 2-x or 25x eyepieces is that you have an extremely small field of view compared to 10x eyepieces. So you see a small area in poor resolution. 99% of microscope for use in a professional setting will use standard 10x eyepieces.