The principle of Lowry assay, Biuret assay, and Bicinconinich (BCA) assay protein assays

[@hhijazi6296]

I'm very pleased with this video; You even went so far as to explain why the Lowry method is more sensitive (at around 19:10)

[@hokudoshichisei]

I was browsing around to learn why carbonate buffer extracted proteins cannot be assayed using the more common BCA method. The composition of the BCA reagent presented here seems to give me the answer. Many thanks for this presentation!!

[@Molecular_Machine]

Great job, you're a gem. Gave me the best refresher I could hope for. Keep doing what you do!

[@biomedicalandbiologicalsci4989]

Thank you so mutch .. I will .. and please give me encourage by subscribing the channel and sharing the videos ;)

[@pitou6084]

@@biomedicalandbiologicalsci4989 very helpful, thank you for your efforts. Can you make a video about MSD meso scale?

[@VyTran-ew9yi]

This was so so helpful. Thank you immensely for this, made my introduce to BCA assays so much easier! Now I can write my lab report with slight more confidence!!

[@alaina8314]

Super helpful before our Biuret and BCA Assay lab today. Thanks

[@sabnym3308]

hi again :) thanks for your answers, some things on the lowry are still not clear to me, the internet couldnt help, maybe you can: biuret: copper2+ and peptide becomes a complex. we talk about copper II (same as 2+). so this copper can offer 2 places for electrons to the Ns of the peptide. Thats how the complex is built. in the complex copper is still 2+. how does it come, it changes to copper 1+ ? who or what is reducing the copper? and when it became copper 1+, how can it still be in the complex? because in the complex it should always be copper 2+, not copper 1+. thank you very much for your time and your help!

[@violets3]

Ever find an answer to this?

[@maggiejameel6725]

Really well explained, please keep on posting!

[@lonely_guy294]

Thank you please continue your good work

[@greendayrockism]

Congratulations on this video! It was extremely useful and very well explained :)

[@biomedicalandbiologicalsci4989]

Thank you stay tuned .... :)

[@johntindell9591]

Thank you so much.

[@moniashek2137]

thank you so much for the video, extremely helpful

[@biomedicalandbiologicalsci4989]

Thank you for the encouragement, stay around, new videos are always coming up ..

[@moniashek2137]

you literally saved my assignment, it's due today :) I will, lab forever :D

[@shuanglongzhang8374]

thank you so much for the video.

[@norma1463]

Great Explanation. Thank you.

[@aayushphadte]

This video really helped me alot!!! Thank you 😁

[@kusumbhalla6760]

Very nice and knowledgable video with easy explanation

[@sofiamata3507]

Fantastic work!! Sorry if it’s inappropriate but could I have your last name to reference you work on my lab report? Otherwise I can’t use what I learned here for my work

[@atpsmpss4201]

In the Lorry assay: the BSA concentracion to make de calibration curve can be 10mg/ml or we need a lower concentration?

[@shreenandansahu]

Loved the way you explained

[@ozeiasbatistadossantos119]

Great content! What about the phenylalanine? Is it oxidized by the Folin-Ciocalteu reagent also?

[@fredanir]

thanks, well explained!

[@soussouadrianna7732]

Thank you a lot ... Thank you so much for the nice video... I Can understand the reaction

[@sushmita1436]

Beautifully explained :)

[@biomedicalandbiologicalsci4989]

Thank you :D

[@Kwan4289-t6]

Thank you so much to share this video, it's very useful for me, I wish you do more video

[@sarahfalcone8716]

Great explanation, thank you!

[@hashtaghealthy_ger6253]

Thank you so much for this video! You helped me a lot understanding this process :)

[@kuffuor_godfred]

Thank you, well explained.

[@rishikeshjaiswal3979]

THANK YOU!

[@puhtreecuh6679]

love your explanation! I completely understand what is going on now! thank you!

[@loucas6065]

18.54 shouldn't the absorbance be at 500nm where the blue colour is in the visible spectrum? instead of 750nm

[@logan2811]

Our eyes see reflected light off objects, if it absorbed blue we would not see it as blue. The color it absorbs makes the solution seem blue.

[@lilithasotondoshe8226]

This was the best video ever

[@syedanoshhashmi2050]

Really very helpfu. Thanks Alot

[@luisvieira7862]

PERFECT! Thanks a lot!

[@karlamel216]

You explain very well, thanks for the video. New subscriber.

[@shadowboy84]

Thanks for the explanation 🌸

[@ngassajuliusmussachallya]

thanks for a good lesson it is so helpuful

[@Mohitthakurofficial]

Amazing! You made Lowry assay on my request. Thank you very much :) Keep it up! please make videos on method and mechanism of Western Blotting technique and Chromatographic techniques.

[@biomedicalandbiologicalsci4989]

Ya true this video was made on your request ... and I will try also to make videos on western blotting and chromatography techniques ... stay around

[@Mohitthakurofficial]

That's so kind of you. Thanks again for such amazingly explanatory videos.

[@biomedicalandbiologicalsci4989]

I saw your comment on the SDS-PAGE video asking for 2D gel electrophoresis, but I could not find it then ... Here is the link: kzbin.info/www/bejne/gKKpnqGufLKWaMk

[@akankshabasnath4341]

Could anyone explain in short the mechanism of Lowry method ?

[@deborupbhowmick4919]

Nice understable lecture❤️

[@SKY92NET]

Awesome 👏

[@abdurrahmanabdurrahman9836]

So fantastic and fascinating video we need more type videos like this I really enjoyed it

[@offside2066]

always appreciated this video thank you so much, really thankful to you....

[@naturethetherapist4422]

Really very grateful understanding vdyo

[@wilmayuangu9386]

Very helpful thank you Keep making vedio

[@joseg.9150]

Great video!!!

[@KaushalGrover]

Thank you🤗🤗

[@rikkigupta9546]

Thank u so much explained in easy manner

[@dewipujiastuti4458]

terima kasih bund:)

[@tiaraflorindaamandita1231]

🥲👍🏻

[@ambertjeify]

Are there any references?

[@lindascavo4158]

i'm working with BCA assay , when i calculate the concentration of protein unknow from the formula of curve , i must multiplie with the dilution factor , how much is the dilution factor in this case and how you have calculated ?? thans to your answer

[@lostthetrainerofdemons5379]

this is an amazing video

[@lostthetrainerofdemons5379]

it wont stick in my head;-;

[@biomedicalandbiologicalsci4989]

Thank you ... stay in the channel many interesting videos are coming up :)

[@Abdullah_Khaled]

Thank you very much

[@JemimaTomlinson321]

if the lowry assay remained yellow can u explain why that wouldve happened

[@achmadhanifmardinsyah5825]

in lovvry assay.... in frist step you add C(2+), so if there no protein there still molibdenum blue formed right ? because C(2+) unreacted vvith peptide bond can reduce folin reagent

[@sakthivelkarthick2226]

Really a nice video

[@lakshmirao7935]

Is fibers proteins which are insoluble in water give this test. Why dipeptides do not give this test. Is it essential to have peptide nitrogens only, terminal amino groups do not form this test. Pl comment. Regards.

[@asenopaphino8230]

Thank you... it's extremely good and helpful...easy to catch up...

[@biomedicalandbiologicalsci4989]

Thank you for your comment .. stay tuned :)

[@rajatsansaniwal3205]

Amazing!

[@kaiquealves1397]

Thank you. It was very clear and useful!

[@maheshkalshetty9850]

super and short explanation but if diagrams was present its very good.....

[@biomedicalandbiologicalsci4989]

Thank you for your comment ... and next time I will take care of the diagram issues ..

[@garrythesnail1900]

Really helpful. 👏👏👏 Good going. Great speech. Awesome try !

[@sudevpradhan392]

really helpful

[@thebiobuddy9595]

Excellent

[@park6768]

awesome!

[@VijayRampurkar]

Thanks amazing job .............................

[@daily-learning-with-me]

very helpful

[@shinyshrooms3841]

Great Video! :)

[@matildesanna6895]

peccato per i sottotitoli in italiano.... tutti sbagliati

[@tajamul1535]

Nice vedio

[@nassim505]

❤️❤️❤️❤️❤️❤️

[@gowthamvenkatchalam5312]

Can we have a sprout seed with protein concentration of 55 mg/ml?

[@Nitro0xide]

thanks my nigga

[@mohameddegm5277]

Thank you very much The video is wonderful and helpful I want your personal profile if possible

[@lokeshkishor7904]

its helpful

[@hassie5294]

Hello, i want your name is for my university report, so i want to put your name on my references....pls great video

[@anjubala4931]

Helpful :)

[@muratozcae]

Where are my Turkish fans ? Only me ? :D