Total Phenol Content (Procedure and Calculation)

Total Phenol Content (Procedure and Calculation)
Performed by Mohammad Shah Hafez Kabir
Founder and CEO, GUSTO A Research Group
Chief Trainer at SAGE Research Training Centre
B. Pharm, Department of Pharmacy
Faculty of Science and Engineering
International Islamic University Chittagong (IIUC)
Google Scholar: scholar.google...
ResearchGate: www.researchga...
Facebook: / ashfing
Relevant Publications:
1. Mohammad Nazmul Islam, Mohammad Shah Hafez Kabir, Syed Md. Abdul Kader, Mahmudul Hasan, Ebrahim Khan Samrat, Imran Bin Habib, Mohammad Nazmul Hoque Jony, Mohammed Sohel Chowdhury, Abul Hasanat, Md. Masudur Rahman. Total phenol, total flavonoid content and antioxidant potential of methanol extract of Boehmeria platyphylla d don leaves. World Journal of Pharmaceutical Research 05/2016; 5(5). DOI:10.20959/wjpr20165-6159
2. Tanvir Ahmad Chowdhury, Mohammad Shah Hafez Kabir, Syed Md. Abdul Kader, Mahmudul Hasan, Md. Ariful Islam, Md. Rakibul Hasan, Md. Nazmul Huda, Shefatun Nur, Joy Chakraborty, Abul Hasanat, Md. Mominur Rahman. Total phenol, total flavonoid content and antioxidant activity of methanol extract of Lygodium palmatum (Bernh.) Sw. leaves. International Journal of Pharmacy 06/2016; 6(2).
3. Kabir MS, Hossain MM, Kabir MI, Ahmad S, Chakrabarty N, Rahman MA, Rahman MM. Antioxidant, antidiarrheal, hypoglycemic and thrombolytic activities of organic and aqueous extracts of Hopea odorata leaves and in silico PASS prediction of its isolated compounds. BMC Complementary and Alternative Medicine. 2016 Nov 21;16(1):474.
4. Mohammad Shah Hafez Kabir, Mohammed Munawar Hossain, Md. Imtiazul Kabir, Md. Mominur Rahman, Abul Hasanat, Talha Bin Emran, Md. Atiar Rahman. Phytochemical screening, Antioxidant, Thrombolytic, α-amylase inhibition and cytotoxic activities of ethanol extract of Steudnera colocasiifolia K. Koch leaves. Journal of Young Pharmacists 10/2016; 8(4). DOI:10.5530/jyp.2016.4.15

[@busithe1st]

Wrote the caption in Engish but presented the content in native language... 🤷‍♀️

[@raziyasultana7944]

Thanks , i was so confused but now its clear. Thanks bro

[@amrakhan9863]

Raziya sultana can you please help me i am also confuse about Y value

[@jonathanho8813]

Sir, wanna to ask , how can you get the concentration of the gallic acid from the standard curve plotted and it was equal to the 82.67 , as the absorbance of the first sample is 0.281. By using your linear graph equation, substitute the y= 0,281 to the y = 0.0039x + 0.033, i got the answer with x = 63.59.

[@tranquil-sereneplatform751]

My question exactly. I am struggling to understand

[@poojasaklani8472]

Did you get the results?

[@maheshf8054]

Please provide videos with subtitles English to get easily for everyone

[@destade1]

Thank you for your nice explanation!

[@UnmuktaRaj]

Can you share the link of the paper or the file from which you are explaining. It would be really helpful.🙏

[@thatikondaashwini3381]

thank you sir

[@YousefAbusamraHanani]

Your accent is difficult to understand, yet we understand almost all native accents easily, and you are using a music as well!!!!!!! this is too annoying to listeners.. You are making us suffer to understand what you say.. The good thing that we are watching the text in the video... speak clearly .. Finally, thank you for your work and efforts.

[@mountcarmel3523]

An excellent source if it were not that you switched to a language different to English.

[@rahmanishtiaquejoy4223]

You guys are doing great work! don't pay mind to the nay sayers

[@mdm.rahman9890]

Do you have the protocol of determination of total free amino acids in plants?

[@clip012]

3:09 may I know how the plant is extracted? is it water extract or methanol extract?

[@beeta939]

what does it means when it comes to ' specific water was added for 10 times dilution '

[@viharpatil8668]

Multiply ur answer with 10

[@gashawtadele772]

Thank you

[@md.tahjib-ul-arif7222]

Thanks for taking the initiative. Excellent video. Would you please tell me about the extraction procedure. And how you prepare micro g/ml plant extract? Thanks.

[@GUSTOAResearchGroup]

kzbin.info/www/bejne/l4vFmZuvftmliK8

[@md.tahjib-ul-arif7222]

Thanks for your prompt response.

[@aditithakur6767]

Sir i have one confusion What is the purpose of preparing standard solution of gallic acid ? When it is added in the solution of plant extract?

[@sankarasubramanian5053]

Nice understandable segment, but when it comes to data analysis seems to have hitch, because of non usage of English. Please interpret the same in English. Requesting to share the name of the practical book with Total phenol content.

[@user-tl6kg7ih2k]

Thanks

[@user-lg3gg5cx4v]

Can I use Folin 10%?

[@Divine_Love...]

please somebody help me... how to prepare extract using methanol....for determination of phenol

[@leenasharan4299]

your video is easy to understand. Thank you for this video. kindly let me know how to cite this protocol??

[@baochau4877]

Thank you so much for your lesson

[@amrakhan9863]

Can someone please explain why me select the more diluted value of OD for Y

[@Divine_Love...]

could you give the site for this procedure?????

[@1109S-g7u]

i m unable to understand what u r saying ....please use English in clear voice

[@arifshaffiq2412]

hi can I have this word document?

[@GUSTOAResearchGroup]

Please give us your email id. Thank you.

[@christbopenga7936]

Hello I watched your video on the determination of total phenols. I am a little confused. what mass of plant extract did you use for the test. And if you can, you can explain to me how you express the total phenol content in milligrams gallic acid equivalent per milligram of extract.Thank you urgently

[@GUSTOAResearchGroup]

You can see the following book/article: 1. Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. 3rd ed. Springer: Dordrecht, The Netherlands; 1998. 2. Evaluation of Bonamia semidigyna (Roxb.) for antioxidant, antibacterial, anthelmintic and cytotoxic properties with the involvement of polyphenols link: link.springer.com/article/10.1007/s13596-018-0334-x

[@zeinabraslan8288]

Could I have a copy of this word document please?

[@soumipaul9959]

what is x and what is y?

[@imamadriansyah7962]

hello sir, can you tell me how you calculate GAE concentration of your samples?

[@GUSTOAResearchGroup]

Simple absorbance of GA and used calibration

[@charansingpatil5773]

Send soft copy

[@thepositivesoul9867]

nice video but you are requested to record it again in English so that we understand better. It will be even better if you make video of the process. By the way MANY THANKS AND WELL DONE FOR SHARING SUCH VIDEOS

[@kalpanarautela320]

Nicely explained video 🙏 Sir, could you please provide some reference paper for this method, as I will be using your method in my samples . Thank you in advance 🙏

[@beenapal6751]

Sir clear nahi dkh raha h

[@koksinghkushwah9613]

Plz u make next vedio then speak clear

[@ARoy111]

✌✌✌

[@yogeshchouhan4927]

plz send any soft copy of this

[@shareensingh9152]

Plz use Eng

[@anikeshbanik345]

Can ypu send me the pdf or doc file of the document you used to explain the expt.

[@SP-qz3im]

Ye kaun si language hai yaar.... Plz speak in English....

[@kamrulazad4610]

Bengali

[@satyendrayadav784]

please give you email