Seriously??? This is one of the few videos I watched for 33.11 minutes in its entirety. Please, please upload the rest of this series.
@Imjuliapagani4 жыл бұрын
Pro Tropicals I’m excited too.
@AquaMayne4 жыл бұрын
Its up
@VyvienneEaux4 жыл бұрын
This is a very interesting topic and one that is very dear to my heart. But for the 99% of people who are not going to do this at home, I have had success with an alternative that doesn't require aseptic condition: for any plant that can be grown emersed, you can take small cuttings with at least one node and grow them in small terrestrial plant pots of potting soil inside a plastic bag (which is at 100% humidity) under medium lighting conditions. In the event that you don't have much material to work with or the plants are dying, you can increase your success rate with a dip in rooting hormone powder. In plant organogenesis, auxins produce root tips, which then produce cytokinins for shooting, so exogenous cytokinins may not be necessary. The major advantage of tissue culture is that very tiny explants can be used because the plant does not need to have an immune system in aseptic conditions.
@manicmurph2 жыл бұрын
Can you put the explant in clonex and put it in a bag with soil? I really don't have a full idea of this I just take regular clones and use clonex the old school way.
@Handles_AreStupid Жыл бұрын
@@manicmurph Tissue culture is bascially taking a section of a plants node and putting it in PERFECT conditions to grow. Nodes have stem cells which can specialize into any type of cell (stem, leaf, root etc., basically they haven't made their mind up yet). Taking a cutting the traditional way requires at least 3 nodes to have an acceptable success rate which is usually around >60% successful (>80% if using 4 or more nodes). Tissue culture can technically be done with just A SINGLE CELL from a node with a near 100% success rate. The only issue is the risk of contamination, but if you follow sterile procedure, you can literally turn one plant into thousands, which can then be turned into millions... If you want to learn more about this, I would recommend the youtuber "plants in jars". You can even do tissue culture with liquid media if you don't want to deal with the jelly media in this video.
@aslamkajee720 Жыл бұрын
Bro well explained. Experts don't even explain proply in detail what is needed. Well done bro. Sorry 4 years later if coco across this video.
@Frank59213 жыл бұрын
Man this is by far the best culture medium video explaination I have seen. You broke it down such an easy wording that anyone can follow. Thank you so much. You have inspired me to take the leap to tissue culturing of plant. I mean I have been propagating plants by stem cuttings, water culture, and misting. This seems like the nature course to take, so thank you.
@AquaMayne3 жыл бұрын
Thanks for watching, Good luck and have fun!
@SonofTheMorningStar666 Жыл бұрын
Thank you for the comprehensive info. I grow gourmet and medicinal mushrooms as a hobby and have the necessary equipment for this. Was looking for something new to do. Going to watch part two now.
@AquaMayne Жыл бұрын
Glad you found it interesting and helpful, thanks for watching!
@johnsessler6863 ай бұрын
Solid information make appreciate you taking the time to share!
@realvipul Жыл бұрын
😮wow just WOW finally i found someone who explain tissue culture that i can understand. definitely subscribed. 🙏🙏👍👍👍👍
@pauljarvis785610 ай бұрын
Thank you, it’s been many years since my plant tissue culture class in college. This is a great refresher. Also, you might try the microwave to melt your gellan.
@constantmeiring4 жыл бұрын
This is a super useful video for getting started with any sort of plant tissue culture!
@tovekaellander31974 жыл бұрын
This is such a good video, I´ve been wanting to learn tissue culture for years. Please upload the next part! Cant wait to tissue culture my peperomias and monsteras!
@tovekaellander31974 жыл бұрын
just subscribed :)
@rhondacaydenr6002 Жыл бұрын
Thank you so much for sharing your knowledge. I have watched so many videos on this trying to understand the difference in multiplication media and rooting media and you made it so easy to understand. You have done a wonderful job. Best video I've found on this subject.
@robertolesen57823 жыл бұрын
Hey, yup peeps are still watching your excellent video. Great job and thanks for doing it!
@AquaMayne2 жыл бұрын
Thank you too!
@ezequielromero7662 Жыл бұрын
It’s the best video at the moment that I I watch. Thanks soo much for this video . Best regard from Argentina
@AquaMayne Жыл бұрын
You're very welcome!
@MiniatureChickenChannel3 жыл бұрын
I'm 2 years late to the video...but wow...super smart everyday dude explaining in terms I can understand! Very Nice 😍😍 I'll now be stalking the rest of your channel 😎
@yett704 жыл бұрын
This is great! Is one of the few videos that explains well besides illustrating. I also work with plant tissue culture and I think it´s a good idea to do it at home. Congrats!
@mdislam63814 жыл бұрын
Searching for the info, if I can do tissue culture for palm trees. Is it possible to build own low cost tissue for a particular plant/ Please help me with information.
@jaimea.gomeza.35703 жыл бұрын
Thank you very much for your video! It is the more useful video on this subject that I watch!
@MrTipinepe4 жыл бұрын
Wooow, nice video, it's just that I looking for. Pleas, keep us informated. I'll be waiting for the 2nd part. And congrats for the video, thank you so much! 👏👏👏👏
@AquaMayne4 жыл бұрын
Its up
@willutech42152 жыл бұрын
You should start the amazon affiliates program so we can click on your links and make you some money for the supplies Thanks for putting these videos together.
@lysafox37894 жыл бұрын
Wow! Thank You. I am trying tissue culture for the first time this week. You helped me understand more than a few things I was confused on. I look forward to your next video.
@ritesha8050 Жыл бұрын
how did it go?
@valencia81750 Жыл бұрын
Thank you so much for explaining everything so clearly!
@ofer3565 жыл бұрын
I'm just starting my tissue culture project , your video is very helpful!! TNX bro !
@AquaMayne5 жыл бұрын
no problem, thanks for watching!
@ofer3565 жыл бұрын
what is the name of the preservative?
@AquaMayne5 жыл бұрын
Ofer Gens it’s just called PPM (plant preservation mixture)
@piranhaaquatics91995 жыл бұрын
Hii can u help me ? I want to start it ,im from india
@2sneayki4u974 жыл бұрын
Hey guys does anyone has a protocol for the ratio for auxin and cytokinin for Bucephalandra and anubias ? Can’t finde them online
@rolandonyul17803 жыл бұрын
F
@bijanmozafari38262 жыл бұрын
i found an article saying something like 2.0 cytokinin for buce do you want link
@nicoleblythe1946 Жыл бұрын
@@bijanmozafari3826 I do
@timgoodin420535 жыл бұрын
Enjoyed the video, waiting for part 2.
@Casicieloshop3 жыл бұрын
Thank you so much for this! I’m so excited to get started myself
@frostie004 жыл бұрын
This is great! Thank you for sharing this information its really helpful. I hope you have time for a part 2.
@spineless59902 ай бұрын
Great video, you cant' go simplier than this. One question: How much potassium hydrixode you need to disolve the hormones? Can you use sodium hydroxide too?
@michaels57054 жыл бұрын
What a great presentation (minor blips aside). There are so many awful presenters that say nothing and lots of it. I like that you didn't tell us things like, what you ate for dinner, what your dog ate for dinner, what color your underwear is, nothing that wasn't relevant. So many presenters could learn from that alone.
@dantel89954 ай бұрын
why does it have to be that complicated? Can't I just make a system that fertilized water flows bellow the plant to get the nutritiens directly from water? Like a pump running water bellow the plant which is either in a spunge or on a wire mesh? Mainly I am planning to grow carpet plants. So, it is a challenge to grow like this but is it possible?
@UhhPolar3 жыл бұрын
You def grow mushrooms bro!. Nobody knows about the SAB without growing lovely mushrooms hah.
@sparkeyjones62613 жыл бұрын
lol.... I keep planted aquariums, and I grow mushrooms. Just discovered another use for the laminar flow hood.
@Hondaf22a13 жыл бұрын
Bro only video that is informative!! Love you
@AquaMayne2 жыл бұрын
Glad you liked it
@terrylunsford18385 жыл бұрын
Are you using plastic cups? How are they not melting when you pressure cook them. What kind cups are you using? I don’t see the link to purchase ppm or the other ones you mentioned.
@MOON_dozer5 жыл бұрын
I use cups advertized as slime cups on amazon, they are pp5 or something. Its kind of like hardness grading for plastic
@MOON_dozer5 жыл бұрын
sorry posting this message from my other account
@SoundSpliff4 жыл бұрын
Polypropylene aka #5 plastic doesn't melt at high temperatures. Commonly used for food, medical, and scientific purposes. Most #5 containers should be sturdy enough to sterilize multiple times.
@manuelurrutia2034 Жыл бұрын
Im having a hard time finding an alternative to MSP12 from Caisson as its not listed as available, is there an alternative you recommend that contains Kinetin?
@abbycooper53522 жыл бұрын
Super informative! Thanks 🤙 For science!
@jimgeorge7390 Жыл бұрын
IBA is INDOLE-3-BUTYRIC ACID and Indole-3-Acetic Acid is another acid. Can you please let me know which one to select among these two?
@sidhe8933 жыл бұрын
Such a well done video, it’s appreciated thank you!
@AquaMayne2 жыл бұрын
Glad you enjoyed it!
@veen884 жыл бұрын
Can you upload 2nd part.
@AquaMayne4 жыл бұрын
Its up
@mdislam63814 жыл бұрын
Thanks for the essential video you uploaded. I wonder If i can have enough info for growing one of my palm tree
@ArtyMars2 жыл бұрын
Thankyou :] doing my first batch today :D
@artryom96783 жыл бұрын
i have a liquid 10ppm BAP, how much should i add for a 1 litre of water
@elvisfreshly18575 жыл бұрын
where you been bro? upload more frequently!
@AquaMayne5 жыл бұрын
My schedule is crazy and managing two channels. This motivates me to make more though bro, thanks for watching.
@elvisfreshly18575 жыл бұрын
@@AquaMayne of course brotha! keeping the hobby alive. What are your other channels?
@MOON_dozer4 жыл бұрын
@@elvisfreshly1857 SkunkyBass
@mattf17872 жыл бұрын
what kind of cups are those?generic condiment cups?or are they autoclavable?
@valencia81750 Жыл бұрын
Can you tell me how to make the plastic box for growing the tissue culture? Do you keep a fan blowing in it at all times? Do you have to sterilize the fan?
@AquaMayne Жыл бұрын
You can just grow them in a sterilized plastic tote. Technically you can grow them on a shelf if the containers are air tight. (Which they should be)
@adamhubalovsky41354 жыл бұрын
This is awesome! Cant wait for Part 2! Subs!
@turtleman19011 күн бұрын
Would you say it's worth doing this.
@AquaMayne11 күн бұрын
It depends. It’s very time-consuming and very hard not to get contamination to be honest.
@julianresurreccion35384 жыл бұрын
Hey bud, i learned alot from your video. When is part 2?
@AquaMayne4 жыл бұрын
Tomorrow
@RodelioVelasco3 жыл бұрын
Thank you very much for sharing this knowledge of yours. I will follow your procedures. I am actually in New Zealand and harder for me to find those chemicals but will try e-bay. Anyway I have a question regarding the protocol, plant type and ratio of hormones for rooting and for multiplication. Where can I fine this protocol? Hard to find it on google. I will experiment tissue culture on citrus.
@shannondelima30283 жыл бұрын
How did you mix the cytokinin, 6-BAP? Did you use distilled water, or a chemical?
@thomasfraser12242 жыл бұрын
Hey really great videos so detailed and easy to understand I think they have given me a good base to give tissue culture a shot just wondering what containers you use to pressure cook your media in they look like plastic but I thought plastic containers would melt
@gooby9306 Жыл бұрын
pp5 is safe at pressure cooker temps
@juanpabloacosta52944 жыл бұрын
Please continue with the series of videos!
@Sahity4 жыл бұрын
Can you post the link to multiplication medium C? could not find it, my english is to blame.
@davida4635 жыл бұрын
Great video! Please upload the next step.
@sarar245210 ай бұрын
Nice video! Where do you get tiny containers that are autoclave safe?
@DarshanSubbaMiraks4 жыл бұрын
I was wondering if this same formula work with Philodendrons ?
@superman2000214 жыл бұрын
Mirak Subba, is there a reason you can't propagate a bunch of cuttings. Or do you want a ton of plants? I see no reason why this wouldn't work for philodendrons, although you would probably get better results with different levels of plant hormones. One thing you could try that would be simpler is to spray your plants with just the hormones with a high BAP ratio to produce more shoots then propagate cuttings in a high IBA ratio solution to encourage root growth.
@angeloborges76843 жыл бұрын
When we bring tissue plants from pet stores. Can we plant smaller amounts of cut plants in Jelly left instead of throwing it out. Will small cut plants put start rooting to make mant plants?
@AquaMayne3 жыл бұрын
Hello. No because the jelly will no longer be steril after you open it. It will grow mold.
@zaman80325 жыл бұрын
very well done.
@beastly_brisket_man62492 жыл бұрын
What is the shelf life of tissue cultured plants?
@piranhaaquatics91995 жыл бұрын
I want to start tissu culture diy project ,can u help me bro ?
@reyhanehkamali7014 жыл бұрын
Thank you
@terrylunsford18384 жыл бұрын
Where can I find the medium C that has everything in it?
@4ll4ll Жыл бұрын
how are you pressure cooking those plastic cups without melting
@AquaMayne Жыл бұрын
I believe it needs to be pp5 rated plastic and it won’t melt.
@MiniatureChickenChannel3 жыл бұрын
OKAY.....does anyone know if it's safe to snap your lids on type when pressure cooking your media? OR do you want to leave them loose? TIA
@AquaMayne3 жыл бұрын
loose, then snap on immediately when done
@MiniatureChickenChannel3 жыл бұрын
@@AquaMayne Thank you sir!!! I appreciate the advice...
@ricks29074 жыл бұрын
Props and great content thanks
@danhassler65858 ай бұрын
I'm having trouble finding some good plastic cups, anyone have any sources?
@AquaMayne8 ай бұрын
amzn.to/3O3PZRr
@tinapitts62223 жыл бұрын
Thank you for this....
@AquaMayne2 жыл бұрын
No problem 😊
@nightcrawler7313 жыл бұрын
Thankyou for sharing this
@AquaMayne2 жыл бұрын
You bet!
@ianeduria42683 жыл бұрын
May I know the brand of your plant preservation mixture?
@kyriptos5 жыл бұрын
Aqua Mayne: The Movie
@aquaessentials4 жыл бұрын
Any update on the next vid? Part 2?
@darrylhosford72892 жыл бұрын
Where do you (or other viewers) source your cups that are heat safe?
How much sucrose to use if I dont have sucrose in my media?
@AquaMayne Жыл бұрын
Hello. I’ve always bought media with Sucrose , so I don’t know.
@videostyle36333 жыл бұрын
Good job bro Is it possible to use murashige and skoog media instead of preparing the nutrient medium? Some of the material are not available and importing them costs a huge amount Thanks
@AquaMayne3 жыл бұрын
video style hi yes , I’m using MS media in this vid
@ironmaiden12369 Жыл бұрын
. YOU ARE A BOSS thx
@AquaMayne Жыл бұрын
Thanks for watching!!! :)
@jaqhass7 ай бұрын
The bleach available in stores is diluted so customers don't commit accidental retroactive self abortion the moment they open the container. Some brands have been known to carry more intense bleach so if you try a new brand 20% could potentially damage the plant. 5-10% should also be fine.
@roshanbande24994 жыл бұрын
Thank you bro... Please upload the second part... I have subscribed your channel..
@robertl67114 жыл бұрын
I can be wrong but I think if you ph the same water at different temps you will get different readings. but I could be wrong.
@SimoWill753 жыл бұрын
This pH meter (I have the same one) has temperature compensation up to 60°C
@carlanthonycalilung57043 жыл бұрын
What do you mean by 20% bleach?
@jprebelocoelhopalma5 жыл бұрын
Nice video bro! But where´s the part 2??
@AnthoNoar4 жыл бұрын
Part 2 pliz :'(
@AquaMayne4 жыл бұрын
Late but its up lol
@paigeart49643 жыл бұрын
Hey did you figure out if the plant anti fungal was worth it for less contamination or not?
@AquaMayne2 жыл бұрын
I believe it does but expensive
@mud34553 жыл бұрын
where do you get your supplies>?
@terrylunsford18385 жыл бұрын
I don’t see the link to purchase the Cytokines,PPM, ect...
@AquaMayne5 жыл бұрын
Check phytotech labs or caison labs .com
@pedrohertz5 жыл бұрын
You just adjust pH once at room temperature. If you rise the temperature of the solution the pH will drop but once it cools to room temperature pH will return to the same value, unless there are some very volatile elements on it. For instance pure water has a pH of 7 at room temperature, bring it to 100C and pH will be 6,14. Let it cool and pH will return to 7. Nice video. Have you tried to grow on liquid solution? By the way, I wonder how your containers don't explode inside the PC with pressure. In my case the lid is blown so I had to figure out another method.
@SimoWill753 жыл бұрын
This pH meter (I have the same one) has temperature compensation up to 60°C
@Labas__Rytas Жыл бұрын
Great video. How did you determine the 0.5 auxins to 1.5 cytokinin ratio? How does this ratio change when you want to grow roots or store plantlets long term?
@AquaMayne Жыл бұрын
Thanks, It depends on the plant. You can look up protocols for certain plants.
@Labas__Rytas Жыл бұрын
@@AquaMayne Ok so what did you do for this plant in the video when you wanted roots?
@certizo5 жыл бұрын
part 2?
@aryah15134 ай бұрын
Sorry to be a nitpicker but what is 20% bleach??? Are you saying 1 part Clorox brand bleach for 4 parts water? That would be roughly 1.5% sodium hypochlorite which would melt any plant in about 2 minutes... how are you able to do this for 10 minutes? The plants should be totally white.
@AquaMayne3 ай бұрын
Hello 10% bleach is more widely used so yes 1 ml of bleach per 9 ml water. Some plants are more fragile than others when it comes to bleach
@BigBry4n3 жыл бұрын
has anyone tried this on Crytocorynes? whats your ratio?
@Opiumfully4 жыл бұрын
praying for a part 2 :)
@AquaMayne4 жыл бұрын
Tomorrow
@AquaMayne4 жыл бұрын
Its up
@MegaFlaxy4 жыл бұрын
Where do you get your PPM?
@AquariumsToGo3 жыл бұрын
You don't add sugar? Or is it already in your MS media?
@AquaMayne3 жыл бұрын
Phil Kent yeah sucrose is in the media
@Roy-qk8uv5 жыл бұрын
How to mix the multiplication media, should i add DI water
@Roy-qk8uv5 жыл бұрын
For multiplication media should i use DI water for BAP
@AquaMayne5 жыл бұрын
Distilled water for everything
@jhkim52262 жыл бұрын
Have you figured out how to make anti-fungal mixture by yourself? They cost so much :(
@AquaMayne2 жыл бұрын
I haven’t. Would be nice because it’s expensive stuff.
@capitalaquatics55534 жыл бұрын
please do more videos about tips and tricks thank you
@Sahity4 жыл бұрын
is there a part 2 coming up? rs
@techshopcity3 жыл бұрын
Thanksssssssss
@OceanMenace4 жыл бұрын
Can you please do the rest of the series
@OceanMenace4 жыл бұрын
I will pay you
@AquaMayne4 жыл бұрын
I will. There is a shortage on the sterilization supplies I need. Im uploading part two in a week or two.
@AquaMayne4 жыл бұрын
It will be out tomorrow
@riverp90194 жыл бұрын
Merry-stem culture is very easy, you just need that microscope. You don't need to be a scientist to dissect out the merry-stem, it's fiddly but DIY tools are just fine to bump and scrap it free particularly as most aquatic plants are so brittle. The plus side to using meristems instead of other plant tissue is that your plants will be guaranteed virus free, plus of course you need very little material. It doesn't work with waterlilies or we could already have a truly "blue hardy" one by now, the first was lost during a flood in Thailand, sterile and refusing to be propagated by any other means it probably ended up floating around in the Andaman sea.
@chumleyk4 жыл бұрын
Part 2 please!
@aryah15134 ай бұрын
Why make a still air box if it's not sealed? Can't contaminants just float in through the holes? And you really shouldn't rely on sterilizing your hands as they're porous. You should use gloves if you're not gonna keep the box sealed 1000% of the time.
@ianeduria42683 жыл бұрын
Where do we exactly dissolve the Auxin?
@AquaMayne3 жыл бұрын
You dissolve it in a seperate container at a 1ml/mg ratio , so you have a stock to pull from.
@ianeduria42683 жыл бұрын
@@AquaMayne distilled water?
@TheJustintavernier3 жыл бұрын
Do you have a Facebook page or something to keep up with also I need the links
@AquaMayne2 жыл бұрын
Hello, I have insta and tick tock thats about it. May make a fb page in the future