Tutorial - DIY Aquarium Plant Tissue Cultures (Part 1)

[@explantculture]

Seriously??? This is one of the few videos I watched for 33.11 minutes in its entirety. Please, please upload the rest of this series.

[@Imjuliapagani]

Pro Tropicals I’m excited too.

[@AquaMayne]

Its up

[@VyvienneEaux]

This is a very interesting topic and one that is very dear to my heart. But for the 99% of people who are not going to do this at home, I have had success with an alternative that doesn't require aseptic condition: for any plant that can be grown emersed, you can take small cuttings with at least one node and grow them in small terrestrial plant pots of potting soil inside a plastic bag (which is at 100% humidity) under medium lighting conditions. In the event that you don't have much material to work with or the plants are dying, you can increase your success rate with a dip in rooting hormone powder. In plant organogenesis, auxins produce root tips, which then produce cytokinins for shooting, so exogenous cytokinins may not be necessary. The major advantage of tissue culture is that very tiny explants can be used because the plant does not need to have an immune system in aseptic conditions.

[@manicmurph]

Can you put the explant in clonex and put it in a bag with soil? I really don't have a full idea of this I just take regular clones and use clonex the old school way.

[@Handles_AreStupid]

@@manicmurph Tissue culture is bascially taking a section of a plants node and putting it in PERFECT conditions to grow. Nodes have stem cells which can specialize into any type of cell (stem, leaf, root etc., basically they haven't made their mind up yet). Taking a cutting the traditional way requires at least 3 nodes to have an acceptable success rate which is usually around >60% successful (>80% if using 4 or more nodes). Tissue culture can technically be done with just A SINGLE CELL from a node with a near 100% success rate. The only issue is the risk of contamination, but if you follow sterile procedure, you can literally turn one plant into thousands, which can then be turned into millions... If you want to learn more about this, I would recommend the youtuber "plants in jars". You can even do tissue culture with liquid media if you don't want to deal with the jelly media in this video.

[@aslamkajee720]

Bro well explained. Experts don't even explain proply in detail what is needed. Well done bro. Sorry 4 years later if coco across this video.

[@Frank5921]

Man this is by far the best culture medium video explaination I have seen. You broke it down such an easy wording that anyone can follow. Thank you so much. You have inspired me to take the leap to tissue culturing of plant. I mean I have been propagating plants by stem cuttings, water culture, and misting. This seems like the nature course to take, so thank you.

[@AquaMayne]

Thanks for watching, Good luck and have fun!

[@SonofTheMorningStar666]

Thank you for the comprehensive info. I grow gourmet and medicinal mushrooms as a hobby and have the necessary equipment for this. Was looking for something new to do. Going to watch part two now.

[@AquaMayne]

Glad you found it interesting and helpful, thanks for watching!

[@johnsessler686]

Solid information make appreciate you taking the time to share!

[@realvipul]

😮wow just WOW finally i found someone who explain tissue culture that i can understand. definitely subscribed. 🙏🙏👍👍👍👍

[@pauljarvis7856]

Thank you, it’s been many years since my plant tissue culture class in college. This is a great refresher. Also, you might try the microwave to melt your gellan.

[@constantmeiring]

This is a super useful video for getting started with any sort of plant tissue culture!

[@tovekaellander3197]

This is such a good video, I´ve been wanting to learn tissue culture for years. Please upload the next part! Cant wait to tissue culture my peperomias and monsteras!

[@tovekaellander3197]

just subscribed :)

[@rhondacaydenr6002]

Thank you so much for sharing your knowledge. I have watched so many videos on this trying to understand the difference in multiplication media and rooting media and you made it so easy to understand. You have done a wonderful job. Best video I've found on this subject.

[@robertolesen5782]

Hey, yup peeps are still watching your excellent video. Great job and thanks for doing it!

[@AquaMayne]

Thank you too!

[@ezequielromero7662]

It’s the best video at the moment that I I watch. Thanks soo much for this video . Best regard from Argentina

[@AquaMayne]

You're very welcome!

[@MiniatureChickenChannel]

I'm 2 years late to the video...but wow...super smart everyday dude explaining in terms I can understand! Very Nice 😍😍 I'll now be stalking the rest of your channel 😎

[@yett70]

This is great! Is one of the few videos that explains well besides illustrating. I also work with plant tissue culture and I think it´s a good idea to do it at home. Congrats!

[@mdislam6381]

Searching for the info, if I can do tissue culture for palm trees. Is it possible to build own low cost tissue for a particular plant/ Please help me with information.

[@jaimea.gomeza.3570]

Thank you very much for your video! It is the more useful video on this subject that I watch!

[@MrTipinepe]

Wooow, nice video, it's just that I looking for. Pleas, keep us informated. I'll be waiting for the 2nd part. And congrats for the video, thank you so much! 👏👏👏👏

[@AquaMayne]

Its up

[@willutech4215]

You should start the amazon affiliates program so we can click on your links and make you some money for the supplies Thanks for putting these videos together.

[@lysafox3789]

Wow! Thank You. I am trying tissue culture for the first time this week. You helped me understand more than a few things I was confused on. I look forward to your next video.

[@ritesha8050]

how did it go?

[@valencia81750]

Thank you so much for explaining everything so clearly!

[@ofer356]

I'm just starting my tissue culture project , your video is very helpful!! TNX bro !

[@AquaMayne]

no problem, thanks for watching!

[@ofer356]

what is the name of the preservative?

[@AquaMayne]

Ofer Gens it’s just called PPM (plant preservation mixture)

[@piranhaaquatics9199]

Hii can u help me ? I want to start it ,im from india

[@2sneayki4u97]

Hey guys does anyone has a protocol for the ratio for auxin and cytokinin for Bucephalandra and anubias ? Can’t finde them online

[@rolandonyul1780]

F

[@bijanmozafari3826]

i found an article saying something like 2.0 cytokinin for buce do you want link

[@nicoleblythe1946]

@@bijanmozafari3826 I do

[@timgoodin42053]

Enjoyed the video, waiting for part 2.

[@Casicieloshop]

Thank you so much for this! I’m so excited to get started myself

[@frostie00]

This is great! Thank you for sharing this information its really helpful. I hope you have time for a part 2.

[@spineless5990]

Great video, you cant' go simplier than this. One question: How much potassium hydrixode you need to disolve the hormones? Can you use sodium hydroxide too?

[@michaels5705]

What a great presentation (minor blips aside). There are so many awful presenters that say nothing and lots of it. I like that you didn't tell us things like, what you ate for dinner, what your dog ate for dinner, what color your underwear is, nothing that wasn't relevant. So many presenters could learn from that alone.

[@dantel8995]

why does it have to be that complicated? Can't I just make a system that fertilized water flows bellow the plant to get the nutritiens directly from water? Like a pump running water bellow the plant which is either in a spunge or on a wire mesh? Mainly I am planning to grow carpet plants. So, it is a challenge to grow like this but is it possible?

[@UhhPolar]

You def grow mushrooms bro!. Nobody knows about the SAB without growing lovely mushrooms hah.

[@sparkeyjones6261]

lol.... I keep planted aquariums, and I grow mushrooms. Just discovered another use for the laminar flow hood.

[@Hondaf22a1]

Bro only video that is informative!! Love you

[@AquaMayne]

Glad you liked it

[@terrylunsford1838]

Are you using plastic cups? How are they not melting when you pressure cook them. What kind cups are you using? I don’t see the link to purchase ppm or the other ones you mentioned.

[@MOON_dozer]

I use cups advertized as slime cups on amazon, they are pp5 or something. Its kind of like hardness grading for plastic

[@MOON_dozer]

sorry posting this message from my other account

[@SoundSpliff]

Polypropylene aka #5 plastic doesn't melt at high temperatures. Commonly used for food, medical, and scientific purposes. Most #5 containers should be sturdy enough to sterilize multiple times.

[@manuelurrutia2034]

Im having a hard time finding an alternative to MSP12 from Caisson as its not listed as available, is there an alternative you recommend that contains Kinetin?

[@abbycooper5352]

Super informative! Thanks 🤙 For science!

[@jimgeorge7390]

IBA is INDOLE-3-BUTYRIC ACID and Indole-3-Acetic Acid is another acid. Can you please let me know which one to select among these two?

[@sidhe893]

Such a well done video, it’s appreciated thank you!

[@AquaMayne]

Glad you enjoyed it!

[@veen88]

Can you upload 2nd part.

[@AquaMayne]

Its up

[@mdislam6381]

Thanks for the essential video you uploaded. I wonder If i can have enough info for growing one of my palm tree

[@ArtyMars]

Thankyou :] doing my first batch today :D

[@artryom9678]

i have a liquid 10ppm BAP, how much should i add for a 1 litre of water

[@elvisfreshly1857]

where you been bro? upload more frequently!

[@AquaMayne]

My schedule is crazy and managing two channels. This motivates me to make more though bro, thanks for watching.

[@elvisfreshly1857]

@@AquaMayne of course brotha! keeping the hobby alive. What are your other channels?

[@MOON_dozer]

@@elvisfreshly1857 SkunkyBass

[@mattf1787]

what kind of cups are those?generic condiment cups?or are they autoclavable?

[@valencia81750]

Can you tell me how to make the plastic box for growing the tissue culture? Do you keep a fan blowing in it at all times? Do you have to sterilize the fan?

[@AquaMayne]

You can just grow them in a sterilized plastic tote. Technically you can grow them on a shelf if the containers are air tight. (Which they should be)

[@adamhubalovsky4135]

This is awesome! Cant wait for Part 2! Subs!

[@turtleman190]

Would you say it's worth doing this.

[@AquaMayne]

It depends. It’s very time-consuming and very hard not to get contamination to be honest.

[@julianresurreccion3538]

Hey bud, i learned alot from your video. When is part 2?

[@AquaMayne]

Tomorrow

[@RodelioVelasco]

Thank you very much for sharing this knowledge of yours. I will follow your procedures. I am actually in New Zealand and harder for me to find those chemicals but will try e-bay. Anyway I have a question regarding the protocol, plant type and ratio of hormones for rooting and for multiplication. Where can I fine this protocol? Hard to find it on google. I will experiment tissue culture on citrus.

[@shannondelima3028]

How did you mix the cytokinin, 6-BAP? Did you use distilled water, or a chemical?

[@thomasfraser1224]

Hey really great videos so detailed and easy to understand I think they have given me a good base to give tissue culture a shot just wondering what containers you use to pressure cook your media in they look like plastic but I thought plastic containers would melt

[@gooby9306]

pp5 is safe at pressure cooker temps

[@juanpabloacosta5294]

Please continue with the series of videos!

[@Sahity]

Can you post the link to multiplication medium C? could not find it, my english is to blame.

[@davida463]

Great video! Please upload the next step.

[@sarar2452]

Nice video! Where do you get tiny containers that are autoclave safe?

[@DarshanSubbaMiraks]

I was wondering if this same formula work with Philodendrons ?

[@superman200021]

Mirak Subba, is there a reason you can't propagate a bunch of cuttings. Or do you want a ton of plants? I see no reason why this wouldn't work for philodendrons, although you would probably get better results with different levels of plant hormones. One thing you could try that would be simpler is to spray your plants with just the hormones with a high BAP ratio to produce more shoots then propagate cuttings in a high IBA ratio solution to encourage root growth.

[@angeloborges7684]

When we bring tissue plants from pet stores. Can we plant smaller amounts of cut plants in Jelly left instead of throwing it out. Will small cut plants put start rooting to make mant plants?

[@AquaMayne]

Hello. No because the jelly will no longer be steril after you open it. It will grow mold.

[@zaman8032]

very well done.

[@beastly_brisket_man6249]

What is the shelf life of tissue cultured plants?

[@piranhaaquatics9199]

I want to start tissu culture diy project ,can u help me bro ?

[@reyhanehkamali701]

Thank you

[@terrylunsford1838]

Where can I find the medium C that has everything in it?

[@4ll4ll]

how are you pressure cooking those plastic cups without melting

[@AquaMayne]

I believe it needs to be pp5 rated plastic and it won’t melt.

[@MiniatureChickenChannel]

OKAY.....does anyone know if it's safe to snap your lids on type when pressure cooking your media? OR do you want to leave them loose? TIA

[@AquaMayne]

loose, then snap on immediately when done

[@MiniatureChickenChannel]

@@AquaMayne Thank you sir!!! I appreciate the advice...

[@ricks2907]

Props and great content thanks

[@danhassler6585]

I'm having trouble finding some good plastic cups, anyone have any sources?

[@AquaMayne]

amzn.to/3O3PZRr

[@tinapitts6222]

Thank you for this....

[@AquaMayne]

No problem 😊

[@nightcrawler731]

Thankyou for sharing this

[@AquaMayne]

You bet!

[@ianeduria4268]

May I know the brand of your plant preservation mixture?

[@kyriptos]

Aqua Mayne: The Movie

[@aquaessentials]

Any update on the next vid? Part 2?

[@darrylhosford7289]

Where do you (or other viewers) source your cups that are heat safe?

[@AquaMayne]

www.amazon.com/dp/B078NNGZ59?psc=1&ref=ppx_yo2ov_dt_b_product_details yes , autoclavable

[@jimgeorge7390]

How much sucrose to use if I dont have sucrose in my media?

[@AquaMayne]

Hello. I’ve always bought media with Sucrose , so I don’t know.

[@videostyle3633]

Good job bro Is it possible to use murashige and skoog media instead of preparing the nutrient medium? Some of the material are not available and importing them costs a huge amount Thanks

[@AquaMayne]

video style hi yes , I’m using MS media in this vid

[@ironmaiden12369]

. YOU ARE A BOSS thx

[@AquaMayne]

Thanks for watching!!! :)

[@jaqhass]

The bleach available in stores is diluted so customers don't commit accidental retroactive self abortion the moment they open the container. Some brands have been known to carry more intense bleach so if you try a new brand 20% could potentially damage the plant. 5-10% should also be fine.

[@roshanbande2499]

Thank you bro... Please upload the second part... I have subscribed your channel..

[@robertl6711]

I can be wrong but I think if you ph the same water at different temps you will get different readings. but I could be wrong.

[@SimoWill75]

This pH meter (I have the same one) has temperature compensation up to 60°C

[@carlanthonycalilung5704]

What do you mean by 20% bleach?

[@jprebelocoelhopalma]

Nice video bro! But where´s the part 2??

[@AnthoNoar]

Part 2 pliz :'(

[@AquaMayne]

Late but its up lol

[@paigeart4964]

Hey did you figure out if the plant anti fungal was worth it for less contamination or not?

[@AquaMayne]

I believe it does but expensive

[@mud3455]

where do you get your supplies>?

[@terrylunsford1838]

I don’t see the link to purchase the Cytokines,PPM, ect...

[@AquaMayne]

Check phytotech labs or caison labs .com

[@pedrohertz]

You just adjust pH once at room temperature. If you rise the temperature of the solution the pH will drop but once it cools to room temperature pH will return to the same value, unless there are some very volatile elements on it. For instance pure water has a pH of 7 at room temperature, bring it to 100C and pH will be 6,14. Let it cool and pH will return to 7. Nice video. Have you tried to grow on liquid solution? By the way, I wonder how your containers don't explode inside the PC with pressure. In my case the lid is blown so I had to figure out another method.

[@SimoWill75]

This pH meter (I have the same one) has temperature compensation up to 60°C

[@Labas__Rytas]

Great video. How did you determine the 0.5 auxins to 1.5 cytokinin ratio? How does this ratio change when you want to grow roots or store plantlets long term?

[@AquaMayne]

Thanks, It depends on the plant. You can look up protocols for certain plants.

[@Labas__Rytas]

@@AquaMayne Ok so what did you do for this plant in the video when you wanted roots?

[@certizo]

part 2?

[@aryah1513]

Sorry to be a nitpicker but what is 20% bleach??? Are you saying 1 part Clorox brand bleach for 4 parts water? That would be roughly 1.5% sodium hypochlorite which would melt any plant in about 2 minutes... how are you able to do this for 10 minutes? The plants should be totally white.

[@AquaMayne]

Hello 10% bleach is more widely used so yes 1 ml of bleach per 9 ml water. Some plants are more fragile than others when it comes to bleach

[@BigBry4n]

has anyone tried this on Crytocorynes? whats your ratio?

[@Opiumfully]

praying for a part 2 :)

[@AquaMayne]

Tomorrow

[@AquaMayne]

Its up

[@MegaFlaxy]

Where do you get your PPM?

[@AquariumsToGo]

You don't add sugar? Or is it already in your MS media?

[@AquaMayne]

Phil Kent yeah sucrose is in the media

[@Roy-qk8uv]

How to mix the multiplication media, should i add DI water

[@Roy-qk8uv]

For multiplication media should i use DI water for BAP

[@AquaMayne]

Distilled water for everything

[@jhkim5226]

Have you figured out how to make anti-fungal mixture by yourself? They cost so much :(

[@AquaMayne]

I haven’t. Would be nice because it’s expensive stuff.

[@capitalaquatics5553]

please do more videos about tips and tricks thank you

[@Sahity]

is there a part 2 coming up? rs

[@techshopcity]

Thanksssssssss

[@OceanMenace]

Can you please do the rest of the series

[@OceanMenace]

I will pay you

[@AquaMayne]

I will. There is a shortage on the sterilization supplies I need. Im uploading part two in a week or two.

[@AquaMayne]

It will be out tomorrow

[@riverp9019]

Merry-stem culture is very easy, you just need that microscope. You don't need to be a scientist to dissect out the merry-stem, it's fiddly but DIY tools are just fine to bump and scrap it free particularly as most aquatic plants are so brittle. The plus side to using meristems instead of other plant tissue is that your plants will be guaranteed virus free, plus of course you need very little material. It doesn't work with waterlilies or we could already have a truly "blue hardy" one by now, the first was lost during a flood in Thailand, sterile and refusing to be propagated by any other means it probably ended up floating around in the Andaman sea.

[@chumleyk]

Part 2 please!

[@aryah1513]

Why make a still air box if it's not sealed? Can't contaminants just float in through the holes? And you really shouldn't rely on sterilizing your hands as they're porous. You should use gloves if you're not gonna keep the box sealed 1000% of the time.

[@ianeduria4268]

Where do we exactly dissolve the Auxin?

[@AquaMayne]

You dissolve it in a seperate container at a 1ml/mg ratio , so you have a stock to pull from.

[@ianeduria4268]

@@AquaMayne distilled water?

[@TheJustintavernier]

Do you have a Facebook page or something to keep up with also I need the links

[@AquaMayne]

Hello, I have insta and tick tock thats about it. May make a fb page in the future