Understanding the HILIC Separation Method in LC

Hydrophilic interaction liquid chromatography, or HILIC, is a powerful mode of liquid chromatography (LC) that uses polar stationary phases and high-organic initial mobile phase conditions for the separation of polar analytes. Join us as we explore HILIC separations.
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Additional Resources
How to Avoid Common Problems with HILIC Methods | www.restek.com/articles/how-t...
Simplify the Switch to HILIC with Raptor HILIC-Si Columns | www.restek.com/articles/rapto...
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Restek Recommended Tools & Parts
Raptor HILIC-Si LC Columns | www.restek.com/search/analyti...
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[@ClyOrtiz]

What an awesome and super simple preparation of how this works. Thank you!

[@RestekCorp]

You're very welcome!

[@Langustin69]

thank you sire, easy and understandable

[@RestekCorp]

You are welcome!

[@wondimenehserawit2487]

What type of mobile phase and stationary phase are used for separation of microbial metabolites from environmental sample??

[@sunnyupadhyay4273]

Dear sir What the impact of dibasic or monobasic buffer on seperation ...and column care

[@RestekCorp]

The separation of different polar analytes is dictated by the choice of HILIC stationary phase. The method development procedure we presented in the video is an universal approach for any regular silica-based column. As this type of column is not stable under high pH, we do not recommend to use basic buffer for the mobile phases. Again, selecting a suitable HILIC stationary phase and work on the pH range from 2-7 is the key for method development.

[@sureshkasakani289]

I want method development activity in hilic . I want highly non polar column method development