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Download ImageJ from the NIH here: imagej.nih.gov/ij/download.html
3:30 - How to download ImageJ
5:10 - Installation Windows users
8:30 - Installation for Mac users
11:48 - How to open your TIFF file on ImageJ and set rotation & dimensions
19:10 - Recognize your ladder
28:15 - Using the ROI manager and begin drawing and adding lines
40:01 - Saving and accessing your work
43:13 - Extract measurements and save as a .csv file
48:39 - Open file in Excel and generate your standard curve