SDS PAGE Introductory Procedures

[@kweuig]

This video saved my thesis work back in 2014 and 2015. Thank you

[@buharimuhammad5989]

A very explanatory step-wise tutor. Thanks so much

[@mei-lingliber6265]

I felt like I was listening to my gearhead garage dad explain how to change the oil XD

[@johnwilloug2717]

Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME! I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized. Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.

[@celestial4646]

Thanks for assisting with my crisis after lab members went home :D

[@CR-uu1rr]

Comprehensive and clear explanation. Thank you

[@sharlyranilazarus8903]

i have a doubt . should the plates go in the space behind the ears or should they sit above the ears in the electrode assembly unit.

[@John49910110]

Very professional explanation

[@Roughen1991]

So nicely explained, I give this to my project students before they enter the lab :) !

[@sonatinac8514]

9:00 Don't panic,reload about 1mm between the space

[@sonatinac8514]

Of well

[@syed-wd8ib]

what is the advantage of choosing constant voltage over constant current?

[@wmgeorge607]

Thank you for giving us the video version of detailed steps much better than reading texts that is so difficult to understand.

[@shirinhameister3610]

LOL @ 7:21 oh gosh, love it when that happens

[@laurafavaro4565]

Great video! Thanks for sharing!

[@kaysseibrahim6477]

WHEN DID YOU ADD THE GEL?

[@surose5229]

He used a gel that its ready to go.

[@abdullahijamiu8617]

What a constructive presentation

[@HeartofART]

Thanks for this video........keep it up.......

[@Morta2709]

Wonderful Video

[@abdukp8529]

Thanks for upload this useful video

[@justyz3648]

Great video! Very insightful.

[@kechang9214]

I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.

[@booloob]

you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though

[@Yoyo-sy5kl]

You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.

[@cozmonaut4248]

Talk about precision.... ;-)

[@kousaykojan9309]

he didnt don gloves

[@Dnjeb]

awesome

[@NurettinPaçal]

Thanks sir

[@christinamansen8636]

Thank you! This is extremely helpful

[@opposinn]

awesome.. one of the best

[@ahmedazzawi9950]

There are 3 types of SDS PAGE, What's the difference between the 3 types? 1. Native SDS PAGE 2. Reducing SDS PAGE 3. Nonreducing SDS PAGE

[@eliasgerardopardollamocca3656]

IS NOT A TEST SIR

[@reginaday3499]

Nice instruction - but, why are you not wearing gloves!!

[@may007ank]

08:13 That's a good load. Lol

[@虞若宏]

Thanks for this great video!

[@alphonsedhasantony4306]

Helpful indeed!

[@inad1563]

Thank you for the video! It was really helpful :)

[@akshayvikhe9970]

its good

[@matthewkramer597]

hhhhhm neurotoxins... wear your gloves kiddos

[@maryiamishyna]

Тhank you!

[@hangnguyen-wz2hr]

vietsub,please!

[@pomgana993]

This is mine project