Video 10: Western blot

[@melaniesoria767]

🎯 Key points for quick navigation: 00:08 *Metabolic diseases lab* 00:38 *Western blot technique* 01:03 *Protein molecular weight* 01:17 *Antibody-protein interaction* 01:54 *Secondary antibody binding* 02:08 *Fluorescent detection dyes* 02:23 *Antibody host species* 03:07 *Gel membrane transfer* 03:50 *Methanol membrane soaking* 04:21 *Filter pad placement* 05:15 *Air bubble removal* 06:01 *Blocking solution preparation* 07:03 *Non-specific binding prevention* 08:14 *Primary antibody incubation* 09:20 *Membrane washing optimization* 10:01 *Secondary antibody selection* 10:41 *Chemiluminescence reaction process* 11:39 *Protein size marking* 12:46 *Membrane reuse preparation* Made with HARPA AI

[@garymijailramosreyes7093]

Excelente :)