Webinar "Advancing Proteomics Research with the timsTOF Pro - TIMS, PASEF and All Ion"

[@johnterrell2825]

At 31:19, with the MS and MS/MS heat maps, why are there observed signals on the MS/MS plot to the right of the m/z of the MS1 peptide? Is it because the MS1 was multiply charged and the fragments are not leading to a higher m/z?

[@BrukerDaltonics]

Thanks for your question. Yes, parents are multiply charged. The consecutive fragments having lost a charge can then have a m/z ratio higher than the parent ion m/z.

[@johnterrell2825]

@@BrukerDaltonics So to understand why this is unique, is it because typically we isolate MS1 based on m/z as a function of retention time but now we are first separating via chromatography and then further picking the MS1 from the other ions via ion mobililty/CCS prior to MS/MS fragmentation?

[@BrukerDaltonics]

Dear John, thanks for being so interested in our webinar. Could you maybe let us know where you are based and how we could contact you? Then we can take this conversation offline and we could bring you in contact with some of our product specialists. Cheers your Bruker Marketing Team.

[@BrukerDaltonics]

​@@johnterrell2825 Thanks for sharing your email address, we deleted your post that the email address isn’t visible in public anymore. Someone of our Team will get in touch with you. Cheers your Bruker Marketing Team

[@yairtechnologies473]

WoW!